灰枣红点软腐病菌拮抗菌株CN124的发酵培养基优化     

崔建州  牛晓科  侯晓杰  冉隆贤 《河北农业大学学报》2009,32(6)

为了降低灰枣红点软腐病对河南省灰枣的危害,减轻农药残留,探索灰枣红点软腐病的生物防治的途径。本研究以龟裂链霉菌菌株CN124为试材,灰枣红点软腐病菌Btryosphaeria dothidea作为目标防治菌株,采用单因子与均匀试验相结合的方法,通过均匀设计回归分析,筛选出菌株CN124对灰枣红点软腐病菌拮抗作用最佳的发酵培养基配方。结果表明,其最佳配方为:淀粉11.67%,玉米粉2.69%,(NH4)2SO40.22%,CaCO30%,NaCl 0.03%。  相似文献   

葡萄园风障防风固沙效果研究   总被引：1，自引：1，他引：0  

刘俊张东风  于祎飞李敬川牛晓科  汉瑞峰宫英振 《中国农学通报》2014,30(10):251-254

为解决砂质土壤上葡萄生产过程中引起的土壤风蚀问题,以高粱杆、苇席和树枝为风障试验材料,利用风速廓线仪和风蚀盘对设置不同风障的葡萄园内外风速和风蚀量变化进行研究。结果表明葡萄园风障可有效降低葡萄园内风速,不同的风障材料对风速的降低效果不同,高粱杆风障防风效果最好,最高可降低风速38.22%。不同材料风障对葡萄园土壤保护效果不同,高粱杆风障的固沙效果最好,比对照降低了68.43%的风蚀量,风障在防风固沙的同时显著降低了因大风对葡萄叶片及果穗的危害。  相似文献   

利用野皂荚嫁接皂荚及接后管理技术     

李中生  刘晓宁  马天丽  董俊梅  牛晓科  张文燕 《现代农业科技》2013,(15):196+199

总结了野皂荚嫁接皂荚及接后管理技术,包括清棵选树、接穗采集与贮藏、嫁接、嫁接后管理等内容,以期为野皂荚嫁接皂荚及接后管理提供参考。  相似文献   

灰枣红点软腐病病原菌的鉴定   总被引：2，自引：0，他引：2  

牛晓科  崔建州  侯晓杰  陈谟林  冉隆贤 《植物病理学报》2010,40(2):217-221

 The damage and symptoms of jujube fruit soft rot in Xinzheng, Henan Province, were investigated from 2006 to 2008, and the pathogen was identified based on the morphological characteristics and the ITS se-quence of ribosome DNA. The results showed that the aerial mycelium of colony was white in color in the first four days, then turned gray after incubation on PDA for 5-6 d at 25℃ and became black two weeks later. The mycelia grew luxuriantly with velvet character. The pycnidium was flask-shaped with a height of 196.9μm and a width of 213.3μm on the avereage. The conidium was colorless with single cell and had the shape of spindle, its size was (15.0-20.0)μm× (4.5-6.5)μm. The conidiophore was fastigiate. The homology of ITS sequence of ribosome DNA between the tested strain NXK and Botryosphaeria dothidea (GenBank ac-cession number:AJ938005) reached 99.87%, with a difference of only two base pairs. Based on the results of both morphological characters and molecular identification, the pathogen of jujube fruit soft rot in Xinzheng was identified as B. dothidea (Moug. ex Fr.) Ces. et de Not..  相似文献