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对FC株猪源性肠毒素型大肠杆菌致病因子的研究 总被引:1,自引:0,他引:1
FC菌株是一株从腹泻仔猪粪便中分离的肠毒素型大肠杆菌(Enterotoxigenic E.coli,ETEC)。在MRHA反应中,本菌能凝集人O型、豚鼠、马、绵羊、牛、鸡和兔的红细胞,对人O型和豚鼠红细胞有很高的血凝性,血抗K88和K99血清不能抑制其对豚鼠和绵羊红细胞的血凝。在体外小肠上皮细胞吸附试验中,本菌对仔猪小肠上皮细胞具有强烈的吸附作用;透射电镜和扫描电镜观察证实了FC株菌除表面具有一种纤毛样结构外,还能定居在仔猪小肠段。血清学试验结果表明,本菌的O抗原属于O101。K88和987P两种抗血清均不能凝集本菌,而K99和F41抗血清均可凝集。对纯化的FC株菌粘着素抗原作等电聚焦和聚丙烯酰胺凝胶电泳分析,结果表明,该菌的粘着素是由等电点分别为4.61和9.78,分子量分别为29500和17500的两种蛋白质抗原所组成。此外,用乳鼠胃内投服试验和兔肠结扎试验证明,该菌只产生热稳定肠毒素。总之,本菌是一株能产生ST的K99,F41的肠毒素型大肠杆菌。 相似文献
3.
Kaartinen L Löhönen K Wiese B Franklin A Pyörälä S 《Acta veterinaria Scandinavica》1999,40(3):271-278
Five Finnish Ayrshire cows in mid or end-lactation were treated with 40 mg sulphadiazine/kg and 8 mg trimethoprim/kg using intravenous (i.v.), intramuscular (i.m.) and subcutaneous (s.c.) routes. Elimination of sulphadiazine was not affected by the route of administration (median t1/2 4.4-5.0 h) while elimination of trimethoprim was strongly limited by slow absorption from the injection site after s.c. and i.m. administration (median for apparent t1/2 21-25 h) compared to that after i.v. administration (median t1/2 1.2 h; p < 0.05). The median bioavailability of trimethoprim was also decreased, being 37% and 55% after s.c. and i.m. administration, respectively. When i.v. administration was used, trimethoprim concentration exceeded 0.1 mg/l in milk between 0.15-8 h while sulphadiazine concentrations above 2 mg/l were maintained from 0.5-2 h to 8 h. After s.c. and i.m. administration sulphadiazine in milk behaved similar to that after i.v. administration, while trimethoprim time-concentration curves were flat and trimethoprim concentrations were around 0.1 mg/l for an extended period of time (8-12 h). Median Cmax values in milk were only 0.07 mg/l and 0.10 mg/l for s.c. and i.m. administrations, respectively. After s.c. administration, 4 out of 5 cows showed signs of pain. After i.m. administration, 2 of the cows showed clear signs of pain and one had some local tenderness at the site of injection. 相似文献
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Ohne Zusammenfassung 相似文献
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Ohne Zusammenfassung 相似文献
7.
Erzsbet Jns Mria Rgyanszki Jnos Olh Lszl Boross 《Aquaculture (Amsterdam, Netherlands)》1983,30(1-4):145-154
The authors studied the pH, temperature dependence, heat inactivation and the reactive groups of the active centers of proteolytic enzymes extracted from the alimentary canal of silver carp, common carp and sheatfish. In these three fish species activation energy values of enzymes, active in a pH range near neutral (7.5) were nearly identical, i.e.: silver carp: 14 kcal/mol; common carp: 15.4 kcal/mol; sheatfish: 15.7 kcal/mol. Differences were found in heat stability. At 55°C time values for 50% activity loss were 3.8: 1.3 and 14.6 min in the case of silver carp, common carp and sheatfish, respectively. Activities of enzymes, active in a pH range near neutral were inhibited by 82–94% by 10−3M PMSF, and by 2–5% by 10−3M EDTA. Proteolytic enzymes of the three fish species proved not to be homogeneous, but the largest part consisted of seryl-proteinases. 相似文献
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Summary Sorghum shoot fly, Atherigona soccata (Rondani) is an important pest of sorghum in Asia, Africa, and Mediterranean Europe, and host plant resistance is an important
component for the management of this pest. The levels of resistance in the cultivated germplasm are low to moderate, and therefore,
it is important to identify genotypes with different mechanisms of resistance to pyramid the resistance genes. We studied
the antixenosis for oviposition, antibiosis, and tolerance components of resistance in a diverse array of shoot fly-resistant
and -susceptible genotypes. The main plants and tillers of SFCR 151, ICSV 705, SFCR 125, and, IS 18551 experienced lower shoot
fly deadhearts at 28 days after seedling emergence, produced more number of productive tillers. The insects fed on these genotypes
also exhibited longer larval period (10.1–11.0 days compared to 9.3 days on Swarna), lower larval survival and adult emergence
(54.7–67.8 and 46.7–52.2% compared to 73.3 and 60.6% on Swarna, respectively), and lower growth and adult emergence indices
as compared to the susceptible check, Swarna. Physico-chemical traits such as leaf glossiness, trichome density, and plumule
and leaf sheath pigmentation were found to be associated with resistance, and chlorophyll content, leaf surface wetness, seedling
vigor, and waxy bloom with susceptibility to shoot fly and explained 88.5% of the total variation in deadhearts. Step-wise
regression indicated that 90.4% of the total variation in deadhearts was due to leaf glossiness and trichome density. The
direct and indirect effects, correlation coefficients, multiple and step-wise regression analysis suggested that deadhearts,
plants with eggs, leaf glossiness, trichomes on the abaxial surface of the leaf, and leaf sheath pigmentation can be used
as marker traits to select for resistance to shoot fly, A. soccata in sorghum. 相似文献
10.
M.?K.?Dhillon H.?C.?SharmaEmail author B.?V.?S.?Reddy Ram?Singh J.?S.?Naresh Zhu?Kai 《Euphytica》2005,144(3):275-283
Summary The shoot fly, Atherigona soccata is an important pest of sorghum, and host plant resistance is one of the most effective components for managing this pest.
Most of the hybrids grown in India based on milo cytoplasm (A1 cytoplasm) are highly susceptible to shoot fly. Therefore, the present studies were undertaken to evaluate different male-sterile
cytoplasms (CMS) for their relative susceptibility to sorghum shoot fly. Oviposition and deadheart formation were significantly
lower on the maintainer lines as compared to the corresponding male-sterile lines. Among the cytoplasms tested, A4M cytoplasm showed antixenosis for oviposition and suffered lower deadheart formation than the other cytoplasms tested. The
A4G1 and A4M cytoplasms suffered lower deadhearts in tillers than the other cytoplasms. Recovery following shoot fly damage in A4M, A3, and A2 cytoplasms was better than in the other cytoplasms tested. The larval and pupal periods were longer and male and female pupal
weights lower in A4M and A4VzM CMS backgrounds compared to the other CMS systems. Fecundity and antibiosis indices on CMS lines were lower than on the
B-lines. The A4M cytoplasm was found to be relatively resistant to sorghum shoot fly, and can be exploited for developing shoot fly-resistant
hybrids for sustainable crop production in future. 相似文献