Manipulated Mouse Embryos as Bioassay System for Water Quality Control   总被引：1，自引：0，他引：1  

AS Elsheikh  Y Takahashi  M Nagano  H Kanagawa 《Reproduction in domestic animals》2003,38(3):204-208

Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K⁺ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system.  相似文献   

ACE inhibition in goats under fixed‐time artificial insemination protocol increases the pregnancy rate and twin births          下载免费PDF全文

VP Fernandes Neto  MNN Silva  AS Costa  D Kunkel  AS Júnior  LCS Feitosa  MCS Muratori  APR Costa 《Reproduction in domestic animals》2018,53(4):1006-1008

To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology.  相似文献   

Calculation of hydraulic conductivities of horizons in some major soils in Wisconsin     

J.L. Denning  J. Bouma  O. Falayi  D.J. Van Rooyen 《Geoderma》1974,11(1):1-16

Hydraulic conductivity between saturation and a tension of 100 cm water was calculated with moisture-retention data for nine soil horizons and compared with results from in situ measurements with the crust test. Agreement was good for sandy, apedal soil horizons with simple packing voids but only if matching factors were used. Results were unreliable in clayey, pedal soil horizons in which a few relatively large planar and tubular pores determine K in the measured tension range, whereas the greatest fraction of total porosity is composed of fine pores inside peds that hardly contribute to flow. Varying the number of pore classes (n) and the water-filled porosity at saturation made no significant difference in the calculations for the apedal soils, but drastically changed the shape of the calculated curves for the pedal soils. Matching factors based on K_sat measurement had to be used for all studied soil horizons, indicating that Marshall's pore-interaction model never predicted K_sat accurately.  相似文献   

Gonadotropin‐induced Puberty Does Not Impair Reproductive Performance of Gilts over Three Parities     

OHO Eckhardt  SMMK Martins  ME Pinese  FC Horta  AC Rosseto  MA Torres  AFC De Andrade  BBD Muro  CT Marino  PHM Rodrigues  AS Moretti 《Reproduction in domestic animals》2014,49(6):964-969

The aim of this study was to evaluate the reproductive performance of three parities of gilts treated or not treated with gonadotropin to induce puberty. Sixty gilts received 600 IU of equine chorionic gonadotropin (eCG) followed by 2.5 mg of porcine luteinizing hormone (LH) 72 h later. Fifty‐nine other gilts were exposed only to a mature boar for 15 min twice daily. Artificial insemination (AI) was performed at 0, 12 and 24 h after the detection of oestrus, and gestation was confirmed by ultrasound after 35 days. Sows were inseminated at the first post‐weaning oestrus. The total numbers of piglets born, piglets born alive, stillborn, mummified foetuses, as well as pregnancy and farrowing rates were evaluated for each of the three parities. Culling rates, farrowing intervals and weaning‐to‐oestrous intervals (WEI) were also analysed. Mean age at puberty and oestrous manifestation were not significantly different between treatments (p = 0.0639; 179.20 ± 17.52 compared with 173.96 ± 16.94, 91.66% compared with 94.92%) across the experimental period. However, females that underwent puberty induction showed modest increases both in the number of total pigs born and in the number of piglets born alive. In conclusion, puberty induction through exogenous gonadotropin administration in field conditions did not induce a more concentrated first oestrous manifestation, but trended to a modest increase in the number of pigs born alive in the first parity and a reduced culling rate during the first gestation.  相似文献   

Hydrologic pathways and chemical composition of runoff during snowmelt in Loch Vale Watershed,Rocky Mountain National Park,Colorado, USA     

A. Scott Denning  Jill Baron  M. Alisa Mast  Mary Arthur 《Water, air, and soil pollution》1991,59(1-2):107-123

Intensive sampling of a stream draining an alpine-subalpine basin revealed that depressions in pH and acid neutralizing capacity (ANC) of surface water at the beginning of the spring snowmelt in 1987 and 1988 were not accompanied by increases in strong acid anions, and that surface waters did not become acidic (ANC<0). Samples of meltwater collected at the base of the snowpack in 1987 were acidic and exhibited distinct ‘pulses’ of nitrate and sulfate. Solutions collected with lysimeters in forest soils adjacent to the stream revealed high levels of dissolved organic carbon (DOC) and total Al. Peaks in concentration of DOC, Al, and nutrient species in the stream samples indicate a flush of soil solution into the surface water at the beginning of the melt. Infiltration of meltwater into soils and spatial heterogeneity in the timing of melting across the basin prevented stream and lake waters from becoming acidic.  相似文献   

FIRST RECORD OF THE BLACK WIDOW SPIDER IN MINNESOTA     

Denning D 《Science (New York, N.Y.)》1937,86(2233):350

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Highly parallel computation     

Denning PJ  Tichy WF 《Science (New York, N.Y.)》1990,250(4985):1217-1222

Highly parallel computing architectures are the only means to achieve the computational rates demanded by advanced scientific problems. A decade of research has demonstrated the feasibility of such machines, and current research focuses on which architectures are best suited for particular dasses of problems. The architectures designated as MIMD and SIMD have produced the best results to date; neither shows a decisive advantage for most near-homogeneous scientific problems. For scientific problems with many dissimilar parts, more speculative architectures such as neural networks or data flow may be needed.  相似文献   

Tackling human fungal infections     

Brown GD  Denning DW  Levitz SM 《Science (New York, N.Y.)》2012,336(6082):647

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Chronic Hypertrophic Ganglioneuritis Mimicking Spinal Nerve Neoplasia: Clinical,Imaging, Pathologic Findings,and Outcome after Surgical Treatment     

Sergio Ródenas DVM Diplomate ECVN  Brian A. Summers BVSc PhD FRCPath  Travis Saveraid DVM Diplomate ACVR  Andrew Denning BVetMed DVDI 《Veterinary surgery : VS》2013,42(1):91-98

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Retrospective analysis of complications and outcomes in Boxers and Staffordshire Bull Terriers undergoing cranial cruciate ligament surgery     

AS Levien  DC Brodbelt  GI Arthurs 《Australian veterinary journal》2013,91(6):220-225

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