One-step continuous coating of silver nanoparticles on well aligned fibers of PAI/PTACM     

Bharat Bajaj  Bin Kim  Sangjun Yoon  Jaerock Lee  Byungki Park 《Fibers and Polymers》2014,15(1):47-56

Highly aligned ultrafine fibers of Poly (amide-co-imide) PAI (torlon)/Poly (trimellitic anhydride chloride-co-4, 4′-methylene dianiline) (PTACM) blends have been prepared by using mechano-electrospinning. Employing a mixed solvent system of DMSO and THF alongwith water coagulation bath as a medium, continuous fibers with improved mechanical properties have been obtained. The continuity of the fibers is strongly dependent on the solvent mixing ratio of DMSO and THF (6:4). Continuous fibers with the most uniform diameter are obtained when a 30 % of PAI and PTACM (1:1) resins by weight is used in the blending solution. The aligned fibers are further coated with silver nanoparticles using a one-step process by passing the electrospunned fibers through PEG solution and a silver precursor solution followed by reduction in a continuous process. The advantage of our method lies in a uniform silver coating on a single fiber that can be coated continuously on a larger length scale (～Km). The mechanical properties of these fibers are dramatically improved after their alignment. Better electrical conductivity is obtained for these fibers after they are coated with silver nanoparticles. The highly aligned silver decorated fibers utilizes a novel electroless continuous process using Polyethylene glycol (PEG), which shows good binding properties and can be used for various bio and electronic applications.  相似文献   

Interspecific hybridization inBrassica juncea × Brassica hirta using embryo rescue     

D. Mohapatra  Y. P. S. Bajaj 《Euphytica》1987,36(1):321-326

Summary Interspecific hybrids have been obtained in an otherwise incompatible cross betweenBrassica juncea × Brassica hirta through the in vitro culture of hybrid ovules and ovaries. The best response was observed from ovules and ovaries cultured 10–15 and 5–7 days after pollination respectively on a basal medium supplemented with indoleacetic acid, kinetin and casein hydrolysate. In some cases the basal cut end of the ovaries proliferated to form callus and shoots. The in vitro-derived hybrid seeds varied in their colour, size and shape, and the F₁ plants in the field showed a large diversity in their morphological traits. The hybrids were sterile, and had an intermediate number of chromosomes (2n=30).  相似文献   

Colorimetric determination of caffeic acid in plant materials   总被引：1，自引：0，他引：1  

K L Bajaj  Y K Arora 《Journal of the Association of Official Analytical Chemists》1979,62(5):1160-1161

A new colorimetric method is described for determining caffeic acid content in plant materials. Caffeic acid is separated by thin layer chromatography from the alcoholic extract, and color is developed using 0.5% aqueous thiosemicarbazide solution under alkaline conditions. The absorbance is read at 475 nm. Lambert-Beer's law is obeyed in the concentration range 0.37-17.5 micrograms caffeic acid/mL. The method is reproducible and has been applied to the estimation of caffeic acid in carrot roots.  相似文献   

Some factors affecting the in vitro propagation of gladiolus     

Y.P.S. Bajaj  M.M.S. Sidhu  A.P.S. Gill 《Scientia Horticulturae》1983,18(3):269-275

Callus tissue cultures have been established from the excised segments of the inflorescence, flower stalks, denuded flower, bract, perianth and leaf segments of 2 cultivars of Gladiolus grandiflorus. Of all the explants and the media tested, the best callus was obtained from the segments of the flower stalks, cultured on a basal medium supplemented with naphthalene acetic acid and kinetin. The callus mostly underwent rhizogenesis, and occasionally differentiated shoots. Complete plants were regenerated from the in vitro cultured cormels, cormel tips and the axillary buds, and 6 plants were formed from the segments of 1 cormel, whereas in nature only 1 plant is obtained per cormel. Cultured young anthers callused and developed leaf-like petaloid structures, and occasionally showed multicellular pollen.  相似文献   

Grafts of porcine small intestinal submucosa seeded with cultured homologous smooth muscle cells for bladder repair in dogs     

Victor JV Rossetto  Lígia SLS da Mota  Noeme S Rocha  Hélio A Miot  Fabrizio Grandi  Cláudia VS Brand?o 《Acta veterinaria Scandinavica》2013,55(1):39

Background

Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.

Results

We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson''s Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.

Conclusions

We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS.  相似文献   

Expression and localization of angiopoietin family in corpus luteum during different stages of oestrous cycle and modulatory role of angiopoietins on steroidogenesis,angiogenesis and survivability of cultured buffalo luteal cells          下载免费PDF全文

SR Mishra  MS Parmar  VP Yadav  R Reshma  J Bharati  MK Bharti  A Paul  VS Chouhan  G Taru Sharma  G Singh  M Sarkar 《Reproduction in domestic animals》2016,51(6):855-869

The objective of this study was to document the expression and localization of angiopoietin (ANGPT) family members comprising of angiopoietin (ANGPT1 and ANGPT2), and their receptors (Tie1 and Tie2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle, and the modulatory role of ANGPT1 and ANGPT2 alone or in combinations on progesterone (P₄) secretion and mRNA expression of phosphotidylinositide‐3kinase‐protein kinase B (PI3K‐AKT), phosphoinositide‐dependent kinase (PDK), protein kinase B (AKT), Bcl2 associated death promoter (BAD), caspase 3 and von willebrand factor (vWF) in luteal cells obtained from midluteal phase (MLP) of oestrous cycle in buffalo. Real‐time RT‐PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors whereas, the P₄ secretion was assessed by RIA. The mRNA and protein expression of ANGPT1 and Tie2 was maximum (p < .05) in mid luteal phase (MLP) of oestrous cycle. The ANGPT2 mRNA and protein expression was maximum (p < .05) in early luteal phase, decreased in MLP and again increased in late luteal phase of oestrous cycle. ANGPT family members were localized in luteal cells and endothelial cells with a stage specific immunoreactivity. P₄ secretion was highest (p < .05) with 100 ng/ml at 72 hr when luteal cells were treated with either protein alone. The mRNA expression of PDK, AKT and vWF was highest (p < .05) and BAD along with caspase 3 were lowest (p < .05) at 100 ng/ml at 72 hr of incubation period, when cultured luteal cells were treated with either protein alone or in combination. To conclude, our study explores the steroidogenic potential of angiopoietins to promote P₄ secretion, luteal cell survival and angiogenesis through an autocrine and paracrine actions in buffalo CL.  相似文献   

Soybean seed coat chitinase as a defense protein against the stored product pest Callosobruchus maculatus          下载免费PDF全文

Nadia CM Silva  Jamile G Conceição  Kayan Eudorico Ventury  Leonardo FR De Sá  Eduardo AG Oliveira  Izabela S Santos  Valdirene M Gomes  Monique N Costa  Andre TS Ferreira  Jonas Perales  Jose Xavier‐Filho  Kátia VS Fernandes  Antonia EA Oliveira 《Pest management science》2018,74(6):1449-1456

  相似文献   

Chemical composition and some plant characteristics in relation to quality of some promising cultivars of carrot (Daucus carota L.)     

K. L. Bajaj  Gurdeep Kaur  B. S. Sukhija 《Plant foods for human nutrition (Dordrecht, Netherlands)》1980,30(2):97-107

Twenty three cultivars of carrot (Daucus carota L.) roots consisting of fifteen local and eight exotic cultivars were analysed for various chemical constituents including dry matter, total soluble solids (TSS), -carotene, total and reducing sugars, phenolics, phosphorus, zinc, manganese, copper, iron, sodium potassium and ash. In addition other characteristics such as days to maturity, root weight and length, flesh thickness, core diameter, number of forked and cracked roots per plot have been studied. Yellow carrots were found to have maximum root length, water soluble carbohydrates and minimum -carotene content. The exotic cultivars were found to have higher TSS content than the local cultivars, while the latter cultivars had higher mineral contents than the former. However, on the average, dry matter content, total water soluble sugars, reducing and non-reducing sugars were found to be greater in exotic cultivars than in local ones, but -carotene, phenols and phosphorus contents were greater in local cultivars. A significant positive correlation between -carotene content, ash percentage and days to maturity was observed. A wide variation in chemical constituents and plant characteristics was observed indicating a high genetic variability in the material under study.  相似文献   

Studies on the development of high-protein biscuits from composite flours     

B. Singh  M. Bajaj  A. Kaur  S. Sharma  J. S. Sidhu 《Plant foods for human nutrition (Dordrecht, Netherlands)》1993,43(2):181-189

Composite flours prepared from wheat, greengram, bengalgram and blackgram flours were studied for the preparation of biscuits. Protein content of biscuits increased as the level of the pulse flours increased. Wheat flour containing bengalgram and blackgram flours adversely affected the top grain, texture and colour of biscuits. Biscuits made with higher levels of bengalgram (more than 15%) were tough and difficult to break and required higher compression force. Addition of greengram flour did not significantly affect top grain, texture and colour of biscuits. The biscuits made from 15% greengram supplemented wheat flour scored the highest for flavour characteristics. Thickness, diameter and spread ratio of biscuits containing different levels of pulse flours were significantly different from control sample. Sensory evaluation scores showed that acceptable biscuits can be prepared from wheat flour supplemented with these pulse flours at a level of 15 percent.  相似文献   

Effect of different levels of nitrogen and farmyard manure on yield and quality of spinach (Spinacea oleracea L.)     

B. D. Kansal  B. Singh  K. L. Bajaj  G. Kaur 《Plant foods for human nutrition (Dordrecht, Netherlands)》1981,31(2):163-170

The effect of different levels of nitrogen N₀(0kg/ha), N₁(30 kg/ha), N₂ (60 kg/ha), and N₃ (90 kg/ha) and farmyard manure F₀ (0 tonnes/ha), F₁ (10 tonnes/ha), and F₂ (20 tonnes/ha) on the yield and nutrient composition of spinach and its uptake was investigated on a sandy loam soil. Yield; phosphorus, iron, manganese, zinc, and copper uptakes; and ascorbic acid content increased with the application of both the inorganic nitrogen fertilizer and the farmyard manure, with a maximum at the N₃F₂ level, i.e. at 90 kg N/ha with 20 tonnes FYM/ha. However, the contents of protein, -carotene, and reducing sugars were maximum at the highest dose of nitrogen without the application of farmyard manure.  相似文献