Production of primary cell cultures on a semi-industrial scale. 1. Modification of the trypsinization procedure--possible means to improve cell yield     

R Beyer  M Rex 《Archiv fuer experimentelle veterinaermedizin》1979,33(4):499-512

To produce large quantities of primary cell suspensions on efficient technique is described which is named "Biomix-Method". By this technique first tissue mince is performed automatically using tissue amounts between 300 g and 500 g. Trypsinization takes place in a three-litre trypsinization vessel using a stirring mechanism according to a defined trypsinization mode. In regard to optimal cell seeding concentration and a justifiable productivity one kidney of newborn or suckling calf yields 6--9 1 cell suspension and that of piglet 1,8-2,3 1, respectively. Proliferation behaviour and effectivity of primary cells are discussed and compared with other cell systems.  相似文献   

Identity of mass-flowering crops moderates functional trait composition of pollinator communities     

Beyer  Nicole  Kirsch  Felix  Gabriel  Doreen  Westphal  Catrin 《Landscape Ecology》2021,36(9):2657-2671

Landscape Ecology - Pollinator declines and functional homogenization of farmland insect communities have been reported. Mass-flowering crops (MFC) can support pollinators by providing floral...  相似文献   

家禽饲料加工与营养之间的相互关系(1)     

R.Scott Beyer  刘瑞征 《饲料广角》2005,(2):32-35

当有人问到饲料加工对家禽营养有哪些重要性时，多数营养学家会谈到颗粒质量的重要性，或谈到某些养分可能在加工过程中受损，但很少会有人将饲料厂看成一个“化学实验室”，其中的热、时间以及反应物等因素综合作用生成最终产品。多年前，饲料厂只是一个将谷物混合做成粉料的场所，而现今，使用新的酶、促进抗体生成的添加剂、  相似文献   

The use of molecular fingerprinting techniques in environmental hygiene     

Beyer W  Böhm R 《Berliner und Münchener tier?rztliche Wochenschrift》1999,112(12):435-443

The analysis of epidemiological pathways for the transmission of pathogens via the environment is a fundamental task in the field of environmental hygiene. The task demands for a precise differentiation between isolates. This can be done by sophisticated techniques for differentiation which are based on phenotypic or genotypic features. The fingerprinting of DNA is a powerful tool in this context. A survey is given for different kinds of RFLP-analysis, plasmid profiling, and PCR fingerprinting as well regarding their principles, methodical variations and published applications. Notable limitations of the applications are also discussed.  相似文献   

Morphological studies in cattle with pathologic-anatomically predominant spleen leukosis     

J Beyer 《Archiv fuer experimentelle veterinaermedizin》1969,23(4):739-764

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Bioenergetics: birth of a bulletin     

R E Beyer 《Science (New York, N.Y.)》1968,162(855):750-751

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The diagnosis of bovine mastitis: a critical evaluation of a polyvalent radial immunodiffusion test and other methods     

W H Giesecke  L W van den Heever  I J du Toit  C E Beyer 《The Onderstepoort journal of veterinary research》1973,40(2):59-67

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A review of toxoplasmosis of animals in the U.S.S.R     

T V Beyer  E A Shevkunova 《Veterinary parasitology》1986,19(3-4):225-243

Information on the serologic and parasitologic prevalence of Toxoplasma gondii infection in domestic and wild animals of the U.S.S.R. is reviewed. Although complement-fixing antibodies to T. gondii were widely prevalent in animals, little information is available on the presence of viable T. gondii in tissues of animals. Reports of epizootics in which T. gondii was suspected to be the causative agent of toxoplasmosis are summarized. Also, the strict economic impact of toxoplasmosis on agriculture in the U.S.S.R. needs evaluation.  相似文献   

Quantitative determination of enterobacteriaceae in foods--anaerobic incubation, no oxidase test     

K Beyer 《Berliner und Münchener tier?rztliche Wochenschrift》1985,98(11):379-382

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Retrieval of Additional Epinephrine from Adrenaclick-Style Epinephrine Autoinjectors     

Arun Ganti  Rebecca Pilkerton  Erika Falsgraf  Elizabeth Vanwert  Nathan Brouwer  Alexander Beyer  Brendan Byrne 《The Forestry Chronicle》2021,32(1):55-58

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