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中草药提取技术的概述   总被引:1,自引:0,他引:1  
简述了中草药的系列提取方法及其应用。其中对101-澄清剂提取法作了较详细的介绍,因为该法是目前应用最广、效果最好、简易可行、经济实用的可替代醇沉法的一种新型提取方法。  相似文献   
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Sow mortality, as the result of pelvic organ prolapse (POP), has been increasing in the last decade in the U.S. swine industry. The objective of this study was to identify potential biological markers associated with risk of POP in sows. We hypothesized that sows differing in perineal score (PS) from PS1–PS3 (PS1—a presumed low POP risk; PS2—a presumed moderate POP risk; and PS3—a presumed high POP risk) would differ in circulatory biomarkers of inflammation and hormonal profiles. On gestation week 15, 2,864 individual sows were assigned a PS, and subsequently, 1.0%, 2.7%, and 23.4% of PS1, PS2, or PS3 sows, respectively, experienced POP. During PS assignment at days 107–116 of gestation, blood samples were collected from sows on two farms of similar genetics, feed sources, and health status. Whole blood was subjected to complete blood count (CBC) analysis (n = 212) and steroid hormones were measured in serum from a subset (n = 110) of animals assigned PS3 parity matched to PS1. Lipopolysaccharide-binding protein (LBP), tumor necrosis factor-alpha (TNF-α), haptoglobin, C-reactive protein (CRP), and creatine kinase (CK) levels were also evaluated. Complete blood count analysis revealed decreased (P ≤ 0.05) mean platelet volume (3.9%), lymphocytes (6.5%), and monocytes (7.5%) in PS3 compared to PS1 sows. Increased (P ≤ 0.02) abundance of androstenedione (13.4%), androsterone (18.2%), estrone (24.8%), and 17β-estradiol (26.2%) was observed in PS3 compared to PS1 sows. Additionally, a 25.8% increase (P = 0.04) in LBP in PS3 compared to PS1 sows was observed. Many dynamic physiological changes occur in sows during late gestation as they approach farrowing. The data presented herein demonstrate that distinct differences in concentrations of circulating biomarkers exist between late gestation sows at high or low risk for POP and may serve as a useful tool for understanding the etiology of POP and evaluation of mitigation strategies.  相似文献   
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Objective To investigate wool organophosphorus concentrations resulting from a range of farm pesticide application methods.
Design Random sampling of wool for pesticide residues and on-farm interviews to determine associated treatments.
Procedure Tasmanian fleece wool lots were sampled at random and tested for organophosphorus residues. The grower was identified and the pesticide treatments applied to the sheep were ascertained by on-farm interview.
Results The residue concentrations showed a large variation that was not accounted for by differences in treatments by growers. Organophosphorus concentrations were proportional to the number of treatments applied, and inversely related to the time between pesticide application and the subsequent shearing, and were significantly influenced by the method of application. After allowing for the time of application, plunge dipping resulted in pesticide residue concentrations 2 to 2.5 times greater than shower dipping, using spray races or hand jetting, and the use of these methods caused larger residues than the use of jetting races.
Conclusions We recommend that plunge or shower dipping should not be used more than 7 weeks after shearing, nor at higher concentration than the standard dose rate used for lice control, whereas jetting may be satisfactory for up to 7 months after shearing, provided only one application is administered.  相似文献   
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Capsaicins, present in most hot sauces and salsas, are responsible for the “hot” sensation in many spicy foods. At high doses, capsaicins cause significant discomfort upon contact with the sensitive tissues of the mouth and throat of mammals. By applying a capsaicin containing paste to the outside of maple tree sap collection tubing, operators hope to minimize rodent (primarily red squirrel, Tamiasciurus hudsonicus) gnawing damage to the tubing. However, some operators and sap processors have expressed concern regarding the potential migration of capsaicins through the tubing and into the tree sap, leading to contaminated maple syrup. To address these concerns, we filled a variety of new and used sap collection tubing with maple sap, plugged the ends, and coated the tubing with a commercially available capsaicin-based rodent repellent paste. Following storage, the contents of the tubes were carefully removed and subjected to a solid-phase extraction clean-up process. Capsaicins in the sap were then quantified by high performance liquid chromatography/fluorescence detection. Results indicate that polyethylene tubing was more resistant to capsaicins migration than was polyvinyl tubing. While capsaicins were detected in the sap, the predicted levels in syrup would be below the human taste threshold.  相似文献   
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