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1.
MCPB-ethyl疏花对富士苹果授粉受精及胚珠发育的影响   总被引:6,自引:1,他引:6  
通过在花期用MCPB-ethyl处理,对富士苹果花粉的发芽、花粉管的伸长以及胚珠的发育等进行了形态方面的观察和探讨,以阐明MCPB-ethyl的疏花机制。结果表明,MCPB-ethyl对花粉的发芽及花粉管的伸长没有影响,整个受精过程与对照相同,没有发现异常。但受精后胚乳核只进行了数次分裂便停止生长,此后珠皮、珠心细胞迅速解体。根据以上结果,认为MCPB-ethyl的疏花效果不是通过影响花粉的发芽或花粉管的伸长阻碍受精所致,而是使胚和胚珠的发育停止,形成离层导致了落花。  相似文献   
2.
基于Web的集成化产品数据管理方法的研究   总被引:1,自引:0,他引:1  
对基于浏览器/服务器(Browser/Server)结构的信息管理方式进行了研究,并提出了基于Web的产品数据管理(PDM)方法,通过引入Web机制,实现集成与共享,以支持先进制造模式。  相似文献   
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用差速离心法提纯PRRS病毒,利用NC膜作为载体,在国内外首次成功建立了检测PRRS血清抗体的斑点酶联免疫吸附试验(Dot-ELISA)。抗原包被浓度为100μg/ml,被检血清工作浓度为1∶10,酶标兔抗猪IgG工作浓度为1∶600。对72份北京地区猪血清分别用Dot-ELISA和IF检测,Dot-ELISA检测阳性率为33.3%,IF检测阳性率为30.6%,与IF符合率较高,对部分待检血清检测结果表明:61份1991年进口美国猪血清阳性率为0%,182份1995年进口加拿大猪血清阳性率为4.94%。本法不需特殊仪器,适用于基层兽医部门和猪场对该病的血清学诊断和流行病学普查  相似文献   
5.
Blood samples from 13 cases of snakebite, 6 in dogs and 7 in cats, were tested for activated partial thromboplastin time (APTT), prothrombin time (PT) and fibrin/fibrinogen degradation products (FDP). Four cases were tested for fibrinogen concentration. Based on the results of a commercially available ELISA test, 9 cases were caused by tiger snakes (Notechis scutatus) and 1 case by a brown snake (Pseudonaja textilis). Three other cases had clinical signs and increased creatine phosphokinase values which suggested tiger snake envenomation. Although the period post-envenomation varied, results indicated a marked prolongation of the APTT and PT in 5 of 6 dogs. Three of these 5 dogs also had increased FDP values and 3 (of 3 examined) were hypofibrinogenaemic. Clinical manifestations of this coagulopathy were: haematoma formation after venepuncture (3 cases), gingival petechiae (1 case) and hyphaema (1 case). In contrast, there was minimal or no prolongation of the APTT and PT values, and no increase in FDP, in all 7 cats. Furthermore, no cat exhibited clinical signs of a coagulopathy.  相似文献   
6.
Hepatitis E virus (HEV) causes 20 million infections worldwide yearly, of which only about 3.3 million are symptomatic. In developed Asian countries, HEV strains detected in human sera and in food sources were genetically similar, suggesting that indigenous HEV infections may be largely food‐borne. To assess the burden of hepatitis E in Singapore, we performed a seroepidemiologic study of the infection. Additionally, we carried out HEV genotyping on archived, residual HEV IgM‐positive serum samples collected between 2014 and 2016 (n = 449), and on pig liver samples (n = 36) purchased from wet markets and supermarkets. Our study shows a rise in hepatitis E incidence (IgM) from 1.7 to 4.1 cases per 100,000 resident population from 2012 to 2016 and an increase in hepatitis E IgG positivity rate among residents from 14% in 2007 to 35% in 2016. Other findings also suggest the epidemiology of hepatitis E in Singapore has shifted, from it being mainly a disease imported from the Indian subcontinent, to one that is now increasingly prevalent in our resident population. Genotypes obtained from 143 human samples identified the majority to be genotype 3 (n = 121), 21 to be genotype 1 and one to be genotype 4. Further phylogenetic analyses suggest genotype 3a to be the cause of indigenous infections in residents, which showed genetic similarity to the genotype 3a strains detected in pig livers. This link between the strains in the majority of human samples and those in pig livers consumed by the public suggests a possible food‐borne route of HEV infection in Singapore.  相似文献   
7.
不同遗传背景的甘蓝型黄籽油菜粒色遗传初步研究   总被引:28,自引:1,他引:27  
以7种不同来源的甘蓝型黄籽油菜品系为材料,采用完全双列杂交试验和与中油821测交的方法,分析了粒色的遗传规律。初步结果显示:不同来源的甘蓝型黄籽油菜品系带有不同的粒色基因,有些为等位基因,有些属非等位基因。粒色遗传以加性效应为主,显性和上位性效应也很明显。  相似文献   
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Modal testing based on the theory of transverse vibration of orthotropic plate has shown great potentials in measuring elastic constants of panel products. Boundary condition (BC) and corresponding calculation method are key in affecting its practical application in terms of setup implementation, frequency identification, accuracy and calculation efforts. To evaluate different BCs for non-destructive testing of wood-based panels, three BCs with corresponding calculation methods were investigated for measuring their elastic constants, namely in-plane elastic moduli (E x , E y ) and shear modulus (G xy ). As a demonstration of the concept, the products used in this study were oriented strand board (OSB) and medium density fiberboard (MDF). The BCs and corresponding calculated methods investigated were, (a) all sides free (FFFF) with one-term Rayleigh frequency equation and finite element modeling, (b) one side simply supported and the other three free (SFFF) with one-term Rayleigh frequency equation, (c) a pair of opposite sides along minor strength direction simply supported and the other pair along major strength direction free (SFSF) with improved three-term Rayleigh frequency equation. Differences between modal and static results for different BCs were analyzed for each case. Results showed that all three modal testing approaches could be applied for evaluation of the elastic constants of wood-based panels with different accuracy levels compared with standard static test methods. Modal testing on full-size panels is recommended for developing design properties of structural panels as it can provide global properties.  相似文献   
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