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G Pugliesi ML Oliveria SC Scolari E Lopes FV Pinaffi BT Miagawa YN Paiva JRG Maio GP Nogueira M Binelli 《Reproduction in domestic animals》2014,49(1):85-91
Strategic supplementation of P4 may be used to increase conception rates in cattle, but timing of supplementation in relation to ovulation, mass of supplementary P4 and formulation of the P4‐containing supplement has not been determined for beef cattle. Effects of supplementation of long‐acting progesterone (P4) on Days 2 or 3 post‐ovulation on development, function and regression of corpus luteum (CL) were studied in beef cattle. Cows were synchronized with an oestradiol/P4‐based protocol and treated with 150 or 300 mg of long‐acting P4 on Day 2 or 3 post‐ovulation (6–7 cows/group). Colour‐doppler ultrasound scanning and blood sample collection were performed from Day 2–21.5. Plasma P4 concentrations were greater (p < 0.05) from Day 2.5–5.5 in the Day 2‐treated groups and from Day 3.5–5.5 in the Day 3‐treated cows than in the control group. CL area and blood flow during Day 2–8.5 did not differ (p > 0.05) among groups, suggesting no effect of P4 treatment on luteal development. The frequency of cows that began luteolysis before Day 15 was greater (p < 0.04) in cows treated with 300 mg than in the controls, but there were no differences between non‐treated and 150 mg‐treated cows. The interval from pre‐treatment ovulation to functional and structural luteolysis was shorter (p < 0.01) in the combined P4‐treated groups than in the control cows. In conclusion, was showed for the first time that long‐acting P4 supplementation on Day 2 or 3 post‐ovulation increases P4 concentrations for ≥3 day, has no effect on luteal development, but anticipates the beginning of luteolysis in beef cattle. 相似文献
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Frank LA Paterson WR Ackerson KL Coroniti FV Vasyliunas VM 《Science (New York, N.Y.)》1991,253(5027):1528-1531
Plasma measurements were obtained with the Galileo spacecraft during an approximately 3.5-hour interval in the vicinity of Venus on 10 February 1990. Several crossings of the bow shock in the local dawn sector were recorded before the spacecraft passed into the solar wind upstream from this planet. Although observations of ions of the solar wind and the postshock magnetosheath plasmas were not possible owing to the presence of a sunshade for thermal protection of the instrument, solar wind densities and bulk speeds were determined from the electron velocity distributions. A magnetic field-aligned distribution of hotter electrons or ;;strahl' was also found in the solar wind. Ions streaming into the solar wind from the bow shock were detected. Electron heating at the bow shock, 相似文献
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The ovarian follicle components must provide an ideal environment to ensure the success of reproductive processes, and communication between follicular cells is crucial to support proper oocyte growth. Recently, it has been demonstrated that the presence of extracellular vesicles (EVs) carrying microRNAs (miRNAs) in follicular fluid represents an important autocrine and paracrine communication mechanism inside the ovarian follicle. In this study, we tested the hypothesis that the miRNA content of EVs isolated from ovarian follicular (granulosa and cumulus–oocyte complexes) cell‐conditioned culture media is dependent upon cell type. We initially screened bovine granulosa cells (GCs) and cumulus–oocyte complexes (COCs), as well as their derived EVs for 348 miRNAs using real‐time PCR, and detected 326 miRNAs in GCs and COCs cells and 62 miRNAs in GCs and COCs EVs. A bioinformatics analysis of the identified cell‐specific and differentially expressed miRNAs predicted that they likely modulate important cellular processes, including signalling pathways such as the PI3K‐Akt, MAPK and Wnt pathways. By investigating the origins of miRNAs within the follicular fluid, the results of this study provide novel insights into follicular miRNA content and intercellular communication that may be of invaluable use in the context of reproductive technologies, diagnostic of ovarian‐related diseases and/or the identification of biomarkers for oocyte and embryo quality. 相似文献
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Scarf FL Coroniti FV Kennel CF Gurnett DA Ip WH Smith EJ 《Science (New York, N.Y.)》1986,232(4748):377-381
The plasma wave instrument on the International Cometary Explorer (ICE) detected bursts of strong ion acoustic waves almost continuously when the spacecraft was within 2 million kilometers of the nucleus of comet Giacobini-Zinner. Electromagnetic whistlers and low-level electron plasma oscillations were also observed in this vast region that appears to be associated with heavy ion pickup. As ICE came closer to the anticipated location of the bow shock, the electromagnetic and electrostatic wave levels increased significantly, but even in the midst of this turbulence the wave instrument detected structures with familiar bow shock characteristics that were well correlated with observations of localized electron heating phenomena. Just beyond the visible coma, broadband waves with amplitudes as high as any ever detected by the ICE plasma wave instrument were recorded. These waves may account for the significant electron heating observed in this region by the ICE plasma probe, and these observations of strong wave-particle interactions may provide answers to longstanding questions concerning ionization processes in the vicinity of the coma. Near closest approach, the plasma wave instrument detected broadband electrostatic noise and a changing pattern of weak electron plasma oscillations that yielded a density profile for the outer layers of the cold plasma tail. Near the tail axis the plasma wave instrument also detected a nonuniform flux of dust impacts, and a preliminary profile of the Giacobini-Zinner dust distribution for micrometer-sized particles is presented. 相似文献
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Gurnett DA Kurth WS Poynter RL Granroth LJ Cairns IH Macek WM Moses SL Coroniti FV Kennel CF Barbosa DD 《Science (New York, N.Y.)》1989,246(4936):1494-1498
The Voyager 2 plasma wave instrument detected many familiar plasma waves during the encounter with Neptune, including electron plasma oscillations in the solar wind upstream of the bow shock, electrostatic turbulence at the bow shock, and chorus, hiss, electron cyclotron waves, and upper hybrid resonance waves in the inner magnetosphere. Low-frequency radio emissions, believed to be generated by mode conversion from the upper hybrid resonance emissions, were also observed propagating outward in a disklike beam along the magnetic equatorial plane. At the two ring plane crossings many small micrometer-sized dust particles were detected striking the spacecraft. The maximum impact rates were about 280 impacts per second at the inbound ring plane crossing, and about 110 impacts per second at the outbound ring plane crossing. Most of the particles are concentrated in a dense disk, about 1000 kilometers thick, centered on the equatorial plane. However, a broader, more tenuous distribution also extends many tens of thousands of kilometers from the equatorial plane, including over the northern polar region. 相似文献
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