Physiological and sanitary condition of the white clam Dosinia ponderosa collected from a coastal area impacted by shrimp farm effluent     

Marcel Martínez-Porchas  Susana Maria Scheuren-Acevedo  Luis Rafael Martínez-Córdova  Teresa Gollas-Galvan  Ramon H. Barraza-Guardado  Fernando Enríquez-Ocaña  Edilmar Cortés-Jacinto  Marco A. Porchas-Cornejo 《Aquaculture International》2016,24(1):243-256

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Total intravenous anesthesia with ketamine–medetomidine–propofol in horses     

MA Umar  K Yamashita  T Kushiro  WW Muir 《Veterinary anaesthesia and analgesia》2005,32(4):18-19

Propofol is a potentially useful intravenous anesthetic agent for total intravenous anesthesia (TIVA) in horses. The purpose of this study was to compare the anesthetic and cardiorespiratory effects of TIVA following the administration of propofol alone(P–TIVA) and ketamine–medetomidine–propofol (KM–P–TIVA) in adult horses. The carotid artery was translocated to a subcutaneous position during TIVA with P–TIVA (n = 6) or KM–P–TIVA (n = 6). All horses were premedicated with medetomidine [0.005 mg kg^–1, intravenously (IV)]. Anesthesia was induced with midazolam (0.04 mg kg^–1 IV) and ketamine (2.5 mg kg IV). All horses were orotracheally intubated and breathed 100% oxygen. The KM drug combination (ketamine 40 mg mL^–1 and medetomidine 0.05 mg mL^–1) was infused at a rate of 0.025 mL kg^–1 hour^–1. Subsequently, a loading dose of propofol (0.5 mg kg^–1, bolus IV) was administered to all horses; surgical anesthesia (determined by horse response to incision and surgical manipulation, positive response being purposeful or spontaneous movement of limbs or head) was maintained by varying the propofol infusion rate as needed. Arterial blood pressure and HR were also monitored. Both methods of producing TIVA provided excellent general anesthesia for the surgical procedure. Anesthesia time was 115 ± 17 (mean ± SD) and 112 ± 11 minutes in horses anesthetized with KM–P–TIVA and P–TIVA, respectively. The infusion rate of propofol required to maintain surgical anesthesia with KM–P–TIVA was significantly less than for P–TIVA (mean infusion rate of propofol during anesthesia; KM–P–TIVA 0.15 0.02 P–TIVA 0.23 ± 0.03 mg kg^–1 minute^–1, p = 0.004). Apnea occurred in all horses lasting 1–2 minutes and intermittent positive pressure ventilation was started. Cardiovascular function was maintained during both methods of producing TIVA. There were no differences in the time to standing after the cessation of anesthesia (KM–P–TIVA 62 ± 10 minutes versus P–TIVA 87 ± 36 minutes, p = 0.150). The quality of recovery was good in KM–P–TIVA and satisfactory in P–TIVA. KM–P–TIVA and P–TIVA produced clinically useful general anesthesia with minimum cardiovascular depression. Positive pressure ventilation was required to treat respiratory depression. Respiratory depression and apnea must be considered prior to the use of propofol in the horse.  相似文献   

ROBERT WILLIAM HEGNER     

Cort WW 《Science (New York, N.Y.)》1942,95(2467):373-374

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Astaxanthin Normalizes Epigenetic Modifications of Bovine Somatic Cell Cloned Embryos and Decreases the Generation of Lipid Peroxidation          下载免费PDF全文

R Li  H Wu  WW Zhuo  QF Mao  H Lan  Y Zhang  S Hua 《Reproduction in domestic animals》2015,50(5):793-799

Astaxanthin is an extremely common antioxidant scavenging reactive oxygen species (ROS) and blocking lipid peroxidation. This study was conducted to investigate the effects of astaxanthin supplementation on oocyte maturation, and development of bovine somatic cell nuclear transfer (SCNT) embryos. Cumulus–oocyte complexes were cultured in maturation medium with astaxanthin (0, 0.5, 1.0, or 1.5 mg/l), respectively. We found that 0.5 mg/l astaxanthin supplementation significantly increased the proportion of oocyte maturation. Oocytes cultured in 0.5 mg/l astaxanthin supplementation were used to construct SCNT embryos and further cultured with 0, 0.5, 1.0 or 1.5 mg/l astaxanthin. The results showed that the supplementation of 0.5 mg/l astaxanthin significantly improved the proportions of cleavage and blastulation, as well as the total cell number in blastocysts compared with the control group, yet this influence was not concentration dependent. Chromosomal analyses revealed that more blastomeres showed a normal chromosomal complement in 0.5 mg/l astaxanthin treatment group, which was similar to that in IVF embryos. The methylation levels located on the exon 1 of the imprinted gene H19 and IGF2, pluripotent gene OCT4 were normalized, and global DNA methylation, H3K9 and H4K12 acetylation were also improved significantly, which was comparable to that in vitro fertilization (IVF) embryos. Moreover, we also found that astaxanthin supplementation significantly decreased the level of lipid peroxidation. Our findings showed that the supplementation of 0.5 mg/l astaxanthin to oocyte maturation medium and embryo culture medium improved oocyte maturation, SCNT embryo development, increased chromosomal stability and normalized the epigenetic modifications, as well as inhibited overproduction of lipid peroxidation.  相似文献   

Genotypic characterization by spoligotyping and VNTR typing of Mycobacterium bovis and Mycobacterium caprae isolates from cattle of Tunisia     

Hela Lamine-Khemiri  Remigio Martínez  Waldo Luis García-Jiménez  Jose Manuel Benítez-Medina  Maria Cortés  Inés Hurtado  Mohammed Salah Abassi  Imed Khazri  Mohammed Benzarti  Javier Hermoso-de-Mendoza 《Tropical animal health and production》2014,46(2):305-311

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The coefficient of dominance is not (always) estimable with biallelic markers     

L.A. García‐Cortés  A. Legarra  M.A. Toro 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2014,131(2):97-104

The genetic relationship among individuals at one locus is characterized by nine coefficients of identity. The coefficients of inbreeding, coancestry and dominance (or fraternity) are just linear functions of them. Here, it is shown how they can be estimated using biallelic and triallelic markers using the method of moments, and comparisons are made with other methods based on molecular coancestry or molecular covariance. It is concluded that in the general case of dominance and inbreeding with biallelic markers, only the coefficients of inbreeding and coancestry can be estimated, but neither the single coefficients of identity nor the coefficient of dominance can be estimated. More than two alleles are required for a full estimation as illustrated with the triallelic situation.  相似文献   

Dendrometric analysis of olive trees for wood biomass quantification in Mediterranean orchards   总被引：1，自引：0，他引：1  

Borja Velázquez-Martí  I. López Cortés  D. M. Salazar-Hernández 《Agroforestry Systems》2014,88(5):755-765

This work focuses on the development of dendrometric algorithms to calculate the volume and total biomass contained in olive trees. This laid the foundation for the use of this methodology as a tool to manage resources from orchards, establishing adequate prediction models for assessing other parameters such as income from raw materials from the cultivation, fruit production, CO₂ sinks, and waste materials (residual wood) used for energy or industry. Dendrometry has traditionally been applied to forest trees. However, little research has been conducted on fruit trees because of their heterogeneous structure. This issue was the first step of this research. For this, the form factors were calculated. This relates to the actual volume of the branch with a model volume, calculated as a revolution solid from the base diameter and length. The shape more approximated to 1 was the cylinder model with a mean value of 0.76 and standard deviation (SD) of 0.23. On the other hand, volume equations were obtained for the branches. The distribution of biomass in the tree was analyzed. It is estimated that 40 % of biomass is located in the stem and 60 % in the crown, and most of the crown biomass is concentrated in the first branches (60 %). Afterwards, occupation factors were calculated to relate the wood volume in the crown to its apparent volume, the mean being 0.005 dm³/m³ and SD 0.0025 dm³/m³. Also, equations for predicting the whole wood in the crown were obtained. In this regard, the best results were obtained when the crown diameter was used (R ² = 0.74). These results could be correlated with the production and quality of the fruit, amount of residual biomass coming from pruning, and LIDAR data, which may indicate a simple, quick, and accurate method for predicting biomass.  相似文献   

Identification and quantification of carotenoids including geometrical isomers in fruit and vegetable juices by liquid chromatography with ultraviolet-diode array detection     

Cortés C  Esteve MJ  Frígola A  Torregrosa F 《Journal of agricultural and food chemistry》2004,52(8):2203-2212

A method was established for the identification and quantification of carotenoids including geometrical isomers in fruit and vegetable juices by liquid chromatography with an ultraviolet-diode array detector, using a C(18) Vydac 201TP54 column. The mobile phase used was the ternary methanol mixture (0.1 M ammonium acetate), tert-butyl methyl ether and water, in a concentration gradient, and a temperature gradient was applied. Retinol palmitate was added as an internal standard. An extraction process (ethanol/hexane, 4:3, v/v) was performed, followed by saponification with diethyl ether/methanolic KOH (0.1%, w/v, BHT) (1:1, v/v) for 0.5 h at room temperature. Seventeen different (cis and trans) carotenoids were identified by UV-vis spectra and retention times in HPLC in the juices analyzed. The analytic parameters show that the method proposed is sensitive, reliable, accurate, and reproducible.  相似文献   

Vision and development in Trichoderma atroviride          下载免费PDF全文

Casas S　 Cortés C　 Ríos M　 Rosales T　 Bibbins M　 Olmedo V　 Herrera-Estrella A 《浙江大学学报(农业与生命科学版)》2004,30(4):390-390

Phototropism, the induction of carotenogenesis and reproductive structures, and resetting of the circadian rhythm are controlled by blue light. Trichoderma is used as a photomorphogenetic model due to its ability to conidiate upon exposure to light. In total darkness, T. atroviride grows indefinitely as a mycelium provided that nutrients are not limiting. However, nutrient deprivation and light trigger the conidiation process. A pulse of blue light given to a radially growing colony induc…  相似文献   

Effect of high-intensity pulsed electric fields processing and conventional heat treatment on orange-carrot juice carotenoids     

Torregrosa F  Cortés C  Esteve MJ  Frígola A 《Journal of agricultural and food chemistry》2005,53(24):9519-9525

Liquid chromatography (LC) was the method of choice for quantification of carotenoids (including geometrical isomers) to evaluate the effects of high-intensity pulsed electric field (HIPEF), a nonthermal preservation method, with different parameters (electric field intensities and treatment times), on an orange-carrot juice mixture (80:20, v/v). In parallel, a conventional heat treatment (98 degrees C, 21 s) was applied to the juice. HIPEF processing generally caused a significant increase in the concentrations of the carotenoids identified as treatment time increased. HIPEF treatment at 25 and 30 kV/cm provided a vitamin A concentration higher than that found in the pasteurized juice.  相似文献