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1.
Moore DP Campero CM Odeón AC Posso MA Cano D Leunda MR Basso W Venturini MC Späth E 《Veterinary parasitology》2002,107(4):303-316
The purpose of the present work was to study the epidemiology of Neospora caninum in beef and dairy herds in the Humid Pampas of Argentina. The seroprevalence of N. caninum was evaluated in 2414 serum samples of cows from beef and dairy farms. An indirect fluorescent antibody test (IFAT) was used to determine specific antibodies. The sera was screened at a dilution >or=1:200 and >or=1:600 in cows with reproductive disease antecedents and without them, respectively. Cows without history of reproductive diseases from nine beef and fifteen dairy farms were grouped according to the percentage (> or or 相似文献
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Lucía M. Campero Javier Moreno-Gonzalo María C. Venturini Gastón Moré Andrea Dellarupe Magdalena Rambeaud Ignacio E. Echaide Beatriz Valentini Carlos M. Campero Dadín P. Moore Dora B. Cano Marcelo Fort Rinaldo A. Mota Marcos E. Serrano-Martínez Carlos Cruz-Vázquez Luis M. Ortega-Mora Gema Álvarez-García 《Tropical animal health and production》2018,50(1):75-84
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1–7) and three enzyme-linked immunosorbent assays (ELISAs 1–3; two in-house and one commercial). Diagnostic performance of the serological tests was calculated and compared according to the following criteria: (1) the “Pre-test information,” which uses previous epidemiological and serological data; (2) the “Majority of tests,” which classifies a serum as positive or negative according to the results obtained by most tests evaluated. Unexpectedly, six tests showed either sensitivity (Se) or specificity (Sp) values lower than 90%. In contrast, the best tests in terms of Se, Sp, and area under the ROC curve (AUC) values were IFAT 1 and optimized ELISA 1 and ELISA 2. We evaluated a high number of IFATs, which are the most widely used tests in Ibero-America. The significant discordances observed among the tests regardless of the criteria employed hinder control programs and urge the use of a common test or with similar performances to either the optimized IFAT 1 and ELISAs 1 and 2. 相似文献
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Ludmila Křížová Zuzana Němcová Kateřina Dadáková Mária Chrenková 《Journal of animal physiology and animal nutrition》2021,105(5):832-840
The aim of the study was to determine the ruminal degradability of dry matter (DM), daidzein, genistein, glycitein and total isoflavones in ground full-fat soybean (GFFS) and solvent-extracted soybean meal (SSBM) using the in sacco method. The experiment was carried out in three replications on ruminally cannulated sheep that were fed twice a day with a diet consisted of hay and supplemental mixture (6:4, DM basis). The nylon bags with 2 g feed samples ground to 2 mm were incubated in the rumen for 0, 2, 4, 8, 16 and 24 h. The effective degradability (ED) of DM, daidzein, genistein, glycitein and total isoflavones was calculated at outflow rate of 0.06 h. The ED of DM in GFFS was 77.8% and was higher than in SSBM being 71.8% (p < 0.001). The ED of daidzein (96.8%) and genistein (93.6%) was higher for SSBM compared with GFFS (93.9% and 92.8%, p < 0.001 and p = 0.003, respectively) while ED of glycitein was lower for SSBM than for GFFS (75.5 and 81.7%, respectively, p < 0.001). All isoflavones in the incubations were extensively degraded in the rumen, and regardless of dietary source, they were almost completely degraded after 16 h of incubation. Further, the disappearance patterns, that is the functions describing the time courses of the analyte disappearance, were assessed. The disappearance patterns of daidzein, genistein, glycitein and total isoflavones were similar and showed greater disappearance of mentioned isoflavones from SSBM compared to GFFS (p < 0.001 for daidzein, genistein and total isoflavones and p = 0.002 for glycitein). The study provides knowledge on the effect of processing on degradability of isoflavones in rumen that can be used to clarify the interrelationship between isoflavones and rumen microbiota. 相似文献
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Dietary supplementation of red alga Pyropia spheroplasts on growth,feed utilization and body composition of sea cucumber,Apostichopus japonicus (Selenka) 
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下载免费PDF全文 A M Shahabuddin Mohammad Nakib Dad Khan Koji Mikami Toshiyoshi Araki Takao Yoshimatsu 《Aquaculture Research》2017,48(10):5363-5372
A feeding experiment was conducted in a closed recirculating system to evaluate the effects of freeze‐dried spheroplasts prepared from Pyropia yezoensis (Ueda) on feed intake, growth and biochemical composition of sea cucumber, Apostichopus japonicus (Selenka). Pyropea spheroplasts (PS) were prepared through enzymatic treatment to break down the complex mixture of polysaccharides cell walls that might be easier for growth energy partitioning. Sea cucumbers were fed‐formulated diets with 10 (Diet 1), 30 (Diet 2) and 50 g/kg (Diet 3) inclusion level of PS. A diet without PS was used as a control (Diet 4). The experiment was conducted for 6 weeks maintaining water temperature 15 ± 1°C, photoperiod 18:06 hours (D:L). Feed was supplied ad‐libitum at 16.00 h once in a day, and the remaining feed and faeces were removed in the next day. Results showed that the highest growth was observed in the 50 g/kg PS diet compared to other treatments. Total weight gain, mean weight gain, net yield, protein efficiency ratio (PER) and protein gain (%) were significantly higher in the 50 g/kg PS diet (p < .05). A significantly higher percentage of energy was allocated for growth in the 50 g/kg PS diet. The highest specific growth rate and feed conversion efficiency (p < .05) were observed in the higher percentage of PS diet. Both the growth performance and biochemical analysis showed that superior growth was observed with increasing levels of PS in the diet. We infer that PS can be used as a new, cheaper feed ingredient in the formulated diet of A. japonicus. 相似文献
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Dad Roux-Michollet Yves Dudal Lucile Jocteur-Monrozier Sonia Czarnes 《Biology and Fertility of Soils》2010,46(6):607-616
Steam soil disinfestation is efficiently used in the field for pre-planting pest control. Providing steam to the soil must
have consequences, either beneficial or detrimental for the soil functioning. We set up a laboratory experiment to quantify
the soil quality dynamics induced by this agricultural practice. In steamed and control soil, we monitored the size, the activity,
and the genetic structure of the bacterial community in the top 2 cm soil every second day over a 10-day period after the
treatment. We also characterized the bioavailable organic matter using fluorescence and ATR-FTIR spectroscopy. We showed that
steaming induced the release of twice as much dissolved organic carbon in steamed soil as in the control soil. This extra
carbon was much less fluorescent and contained a higher proportion of aliphatic compounds (alkyl chains, primary alcohols).
After an initial drop in the bacterial community, we observed a tenfold increase in the number of bacteria, a flush in carbon
mineralization, and genetic structure modification, which could be related to the newly released carbon. Steam treatment showed
strong but quickly reversible impacts on the soil functioning, enabling farmers to sow approximately 1 week after treatment. 相似文献
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Dadáková Kateřina Jurasová Lenka Kašparovský Tomáš Průšová Božena Baroň Mojmír Sochor Jiří 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):472-477
Plant Foods for Human Nutrition - Plant lignans possess several properties beneficial for human health and therefore, increasing their contents in foods and beverages is desirable. One of the... 相似文献
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Yanina P. Hecker Dadín P. Moore Jorge A. Manazza Juan M. Unzaga Ernesto J. A. Späth Lais L. Pardini María C. Venturini José L. Roberi Carlos M. Campero 《Tropical animal health and production》2013,45(7):1645-1647
The aim of this study was to describe the occurrence of antibodies to Toxoplasma gondii and Neospora caninum in dairy sheep from the Humid Pampa region, Argentina. Blood samples from 704 dairy sheep belonging to six flocks were collected. Using a cut off titer of 1:50, an indirect fluorescence antibody test was used. Antibodies to T. gondii or N. caninum were detected in 17.3 % (n?=?122) and 3 % (n?=?21), respectively. All the flocks had at least one seropositive animal to T. gondii but two of them had no seropositive sheep to N. caninum. Fifty-two of 122 (42.6 %) positive samples to T. gondii had antibody titers higher than 1:400. There was a significantly higher proportion of T. gondii seropositive animals in females and older sheep (p?<?0.05). Ten of 21 (52.3 %) positive samples to N. caninum had antibody titers higher than 1:400. This is the first report of seroprevalence of T. gondii and N. caninum in dairy sheep from Humid Pampa, Argentina. Further research is required for a better understanding of the role of toxoplasmosis and neosporosis in dairy sheep in Argentina. 相似文献
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Mohammad Nakib Dad Khan Takao Yoshimatsu Alok Kalla Toshiyoshi Araki Shuichi Sakamoto 《Fisheries Science》2008,74(2):397-404
ABSTRACT: A 56-day feeding trial was conducted to evaluate the effects of Porphyra spheroplasts (PS) as a feed additive on growth, carcass composition and feed utilization of black sea bream. Four experimental diets with or without PS (0, 1, 3, 5% inclusion level) were formulated to determine the proper incorporation level for best growth and utilization using white fishmeal as the principal protein source. Three replicate groups of fish averaging 1.28 ± 0.05 g (mean ± SD) were maintained in flow-through thermo-controlled sea water (32–34 psu, 25°C) aquaria (100 L) under the laboratory conditions (L12 : D12) and fed three times a day at 09:00, 13:00 and 17:00 hours by hand at approximately 3% body weight on each diet. The rearing trial revealed that growth performance (in terms of weight gain, specific growth rate) are almost the same level in all the dietary groups, nevertheless the survival, nutrient utilizations and retentions (feed efficiency, protein efficiency ratio, protein retention rate, lipid retention rate) were significantly higher ( P < 0.05) in a group fed on the diet containing 3% spheroplasts. Further, the fish fed on the diet containing PS had significantly higher ( P < 0.05) crude lipid level in their dorsal muscles and viscera. The fish fed a PS-based diet showed higher level of eicosapentaenoic acid and docosahexaenoic acid (DHA) in viscera and DHA in dorsal muscles. The results obtained in this feeding trial suggest that the optimum dietary PS supplementation level up to 3% for black sea bream can be considered as improved consequence on growth performance, nutrient utilization or body composition were noticed. 相似文献
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