Treatment of canine haemangiosarcoma with suberoylanilide hydroxamic acid, a histone deacetylase inhibitor     

L. A. Cohen  B. Powers  S. Amin  D. Desai 《Veterinary and comparative oncology》2004,2(4):243-248

A case report is presented by describing the treatment of a 12‐year‐old dog – diagnosed with haemangiosarcoma (HSA) – with suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor. The drug was administered orally, on a daily basis, approximately 2 weeks post‐splenectomy at a dose of 3 mg kg^?1. HSA is a lethal malignancy of the endothelium, which is usually disseminated by the time it is diagnosed. Median survival time, usually, is no longer than 80 days. Following treatment with SAHA, no sign of malignant growth could be discerned by means of diagnostic abdominal ultrasound, chest X‐ray or with the help of clinical symptoms, over a period of >1000 days. The precise mechanism by which HDAC inhibitors exert their anti‐cancer effects is uncertain, but evidence suggests that exposure to SAHA generates hyperacetylated chromosomal histones, which, in turn, facilitates the expression of tumour suppressor genes turned off by epigenetic mechanisms during neoplastic transformation of the endothelium.  相似文献   

Characterization of an Indian Bluetongue Virus Isolate by RT-PCR and Restriction Enzyme Analysis of the VP-7 Gene Sequence     

Tiwari AK  Kataria RS  Desai G  Butchaiah G  Bandyopadhyay SK 《Veterinary research communications》2000,24(6):401-409

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Retraction     

Flavell RA  Kaczmarek LK  Badou A  Boulpaep EL  Desai R  Basavappa S  Matza D  Peng YQ  Mehal WZ 《Science (New York, N.Y.)》2005,310(5756):1903

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Deletion of Trpm7 disrupts embryonic development and thymopoiesis without altering Mg2+ homeostasis     

Jin J  Desai BN  Navarro B  Donovan A  Andrews NC  Clapham DE 《Science (New York, N.Y.)》2008,322(5902):756-760

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Linolenic acid-modified chitosan for formation of self-assembled nanoparticles     

Liu CG  Desai KG  Chen XG  Park HJ 《Journal of agricultural and food chemistry》2005,53(2):437-441

Chitosan was modified by coupling with linolenic acid through the 1-ethyl-3-(3-dimethylaminopropyyl)carbodiimide-mediated reaction. The degree of substitution was measured by 1H NMR, and it was 1.8%, i.e., 1.8 linolenic acids group per 100 anhydroglucose units. The critical aggregation concentration (CAC) of the self-aggregate of hydrophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe. The CAC value in phosphate-buffered saline (PBS) solution (pH 7.4) was 5 x 10(-2) mg/mL. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH 7.4) was 210.8 nm with a unimodal size distribution ranging from 100 to 500 nm. A transmission electron microscopy study showed that the formation of near spherical shape nanoparticles had enough structural integrity. The loading ability of hydrophobically modified chitosan (LA-chitosan) was investigated by using bovine serum albumin (BSA) as a model protein. Self-aggregated nanoparticles exhibited an increased loading capacity (19.85 +/- 0.04 to 37.57 +/- 0.25%) with an increasing concentration of BSA (0.1-0.5 mg/mL).  相似文献   

Influence of crossbreeding on the performance of Indian cattle     

N R Bhasin  R N Desai 《The Indian veterinary journal》1967,44(5):405-412

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Evaluation of world castor (Ricinus communis L.) germplasm for resistance to Fusarium wilt (Fusarium oxysporum f. sp. ricini)     

K. Anjani  M. A. Raoof  A. G. Desai 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(3):567-578

Castor (Ricinus communis L.) is an important industrial oilseed crop grown worldwide. Wilt caused by Fusarium oxysporum f. sp. ricini is a devastating disease of this crop. The objective of this research was to identify stable sources of wilt resistance among the global castor germplasm collections available in India for use in cultivar improvement. The global collections comprising 1,779 Indian and 190 exotic accessions from 36 countries were screened against wilt in wilt sick plots at two sites in India in preliminary screening. None of the accessions showed high resistance to wilt, 133 accessions comprising 111 Indian and 22 exotic accessions representing 13 countries exhibited resistance. Thirteen of the 133 resistant accessions were tested further for multiple years in wilt sick plots and glasshouse under controlled artificial inoculation at two sites. All the 13 accessions consistently showed wilt resistance in both wilt sick plot and glasshouse at both sites in multiple years. Eleven of these 13 accessions were from India and two were from former USSR. Evaluation for agro-morphological traits identified high seed yielding and early maturing resistant accessions. Diversity analyses precisely revealed diversity among the resistant accessions. These 13 resistant accessions would be great value as donors of resistance.  相似文献   

Estimating nocturnal ecosystem respiration from the vertical turbulent flux and change in storage of CO₂     

Eva van Gorsel  Nicolas Delpierre  Ray Leuning  Andy Black  J. William Munger  Steven Wofsy  Marc Aubinet  Christian Feigenwinter  Jason Beringer  Damien Bonal  Baozhang Chen  Jiquan Chen  Robert Clement  Kenneth J. Davis  Ankur R. Desai  Danilo Dragoni  Sophia Etzold  Thomas Grünwald  Lianhong Gu  Bernhard Heinesch  Lucy R. Hutyra  Wilma W.P. Jans  Werner Kutsch  B.E. Law  Monique Y. Leclerc  Ivan Mammarella  Leonardo Montagnani  Asko Noormets  Corinna Rebmann  Sonia Wharton 《Agricultural and Forest Meteorology》2009,149(11):1919

Micrometeorological measurements of nighttime ecosystem respiration can be systematically biased when stable atmospheric conditions lead to drainage flows associated with decoupling of air flow above and within plant canopies. The associated horizontal and vertical advective fluxes cannot be measured using instrumentation on the single towers typically used at micrometeorological sites. A common approach to minimize bias is to use a threshold in friction velocity, u_*, to exclude periods when advection is assumed to be important, but this is problematic in situations when in-canopy flows are decoupled from the flow above. Using data from 25 flux stations in a wide variety of forest ecosystems globally, we examine the generality of a novel approach to estimating nocturnal respiration developed by van Gorsel et al. (van Gorsel, E., Leuning, R., Cleugh, H.A., Keith, H., Suni, T., 2007. Nocturnal carbon efflux: reconciliation of eddy covariance and chamber measurements using an alternative to the u_*-threshold filtering technique. Tellus 59B, 397–403, Tellus, 59B, 307-403). The approach is based on the assumption that advection is small relative to the vertical turbulent flux (F_C) and change in storage (F_S) of CO₂ in the few hours after sundown. The sum of F_C and F_S reach a maximum during this period which is used to derive a temperature response function for ecosystem respiration. Measured hourly soil temperatures are then used with this function to estimate respiration R_Rmax. The new approach yielded excellent agreement with (1) independent measurements using respiration chambers, (2) with estimates using ecosystem light-response curves of F_c + F_s extrapolated to zero light, R_LRC, and (3) with a detailed process-based forest ecosystem model, R_cast. At most sites respiration rates estimated using the u_*-filter, R_ust, were smaller than R_Rmax and R_LRC. Agreement of our approach with independent measurements indicates that R_Rmax provides an excellent estimate of nighttime ecosystem respiration.  相似文献   

Definitive‐intent intensity‐modulated radiation therapy provides similar outcomes to those previously published for definitive‐intent three‐dimensional conformal radiation therapy in dogs with primary brain tumors: A multi‐institutional retrospective study     

Nathaniel Van Asselt  Neil Christensen  Valeria Meier  Carla Rohrer Bley  Sarah Laliberte  Valerie J. Poirier  Noopur Desai  Yi Chen  Michelle Turek 《Veterinary radiology & ultrasound》2020,61(4):481-489

Radiotherapy with or without surgery is a common choice for brain tumors in dogs. Although numerous studies have evaluated use of three‐dimensional conformal radiotherapy, reports of definitive‐intent, IMRT for canine intracranial tumors are lacking. Intensity‐modulated radiation therapy has the benefit of decreasing dose to nearby organs at risk and may aid in reducing toxicity. However, increasing dose conformity with IMRT calls for accurate target delineation and daily patient positioning, in order to decrease the risk of a geographic miss. To determine survival outcome and toxicity, we performed a multi‐institutional retrospective observational study evaluating dogs with brain tumors treated with IMRT. Fifty‐two dogs treated with fractionated, definitive‐intent IMRT at four academic radiotherapy facilities were included. All dogs presented with neurologic signs and were diagnosed via MRI. Presumed radiological diagnoses included 37 meningiomas, 12 gliomas, and one peripheral nerve sheath tumor. One dog had two presumed meningiomas and one dog had either a glioma or meningioma. All dogs were treated in the macroscopic disease setting and were prescribed a total dose of 45‐50 Gy (2.25‐2.5 Gy per fraction in 18‐20 daily fractions). Median survival time for all patients, including seven cases treated with a second course of therapy was 18.1 months (95% confidence of interval 12.3‐26.6 months). As previously described for brain tumors, increasing severity of neurologic signs at diagnosis was associated with a worse outcome. Intensity‐modulated radiation therapy was well tolerated with few reported acute, acute delayed, or late side effects.  相似文献   

Prothrombin time and activated partial thromboplastin time using a point‐of‐care analyser (Abaxis VSpro®) in Bennett's wallabies (Macropus rufogriseus)          下载免费PDF全文

BN Nevitt  SK Chinnadurai  MK Watson  JN Langan  MJ Adkesson 《Australian veterinary journal》2016,94(10):384-386

There are few reports of coagulation times in marsupial species. Blood samples collected from 14 Bennett's wallabies (Macropus rufogriseus) under anaesthesia during routine health assessments were analysed for prothrombin time (PT) and activated partial thromboplastin time (aPTT) using a point‐of‐care analyser (POC) (Abaxis VSPro®). The wallabies had an aPTT mean of 78.09 s and median of 78.1 s. The PT for all wallabies was greater than 35 s, exceeding the longest time measured on the POC. Although PT was significantly longer, aPTT was similar to the manufacturer's domestic canine reference range.  相似文献