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排序方式: 共有63条查询结果,搜索用时 187 毫秒
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Faham S Watanabe A Besserer GM Cascio D Specht A Hirayama BA Wright EM Abramson J 《Science (New York, N.Y.)》2008,321(5890):810-814
Membrane transporters that use energy stored in sodium gradients to drive nutrients into cells constitute a major class of proteins. We report the crystal structure of a member of the solute sodium symporters (SSS), the Vibrio parahaemolyticus sodium/galactose symporter (vSGLT). The approximately 3.0 angstrom structure contains 14 transmembrane (TM) helices in an inward-facing conformation with a core structure of inverted repeats of 5 TM helices (TM2 to TM6 and TM7 to TM11). Galactose is bound in the center of the core, occluded from the outside solutions by hydrophobic residues. Surprisingly, the architecture of the core is similar to that of the leucine transporter (LeuT) from a different gene family. Modeling the outward-facing conformation based on the LeuT structure, in conjunction with biophysical data, provides insight into structural rearrangements for active transport. 相似文献
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Hemoperitoneum caused by rupture of a juvenile granulosa cell tumor in an equine neonate 总被引:1,自引:0,他引:1
S L Green T E Specht S C Dowling A J Nixon J H Wilson J B Carrick 《Journal of the American Veterinary Medical Association》1988,193(11):1417-1419
A neonatal foal was examined because of apparent abdominal pain and distention, anemia, and hemoperitoneum. Exploratory laparotomy was performed, and a large spherical mass, which had ruptured, was found in the area of the left ovary. Left salpingo-oophorectomy was performed. The mass was determined to be a juvenile granulosa cell tumor. 相似文献
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Specht EJ 《The Onderstepoort journal of veterinary research》2008,75(1):73-81
The study is the result of analyzing 16895 blood smears of cattle collected at 180 sites in the provinces of Manica, Sofala, Zambézia and Tete in Mozambique. Of the blood smears 73.9% were from Manica, 11.8% from Tete, 8.5% from Sofala and 5.8% from Zambézia; 75.6% of these were collected from smallholder cattle. Infections with trypanosomes were highest in smallholder cattle from Sofala Province with 36.8% of the 872 blood smears examined positive for trypanosomes, and lowest in cattle of commercial farmers in Manica Province with only 6.2% of 2252 blood smears being positive. Trypanosoma congolense was the predominant species, followed by Trypanosoma vivax and Trypanosoma brucei sensu lato. Trypanosoma brucei, which also infects humans, was more frequent in the districts of Buzi, Mutarara and Morrumbala with 15.1%, 10.5% and 9.8% of all examined cattle in 2005 being infected with it, respectively. The results show a significant increase in the infection rate with trypanosomes compared with results obtained in previous years by the Regional Veterinary Laboratory in Manica Province and by the Regional Tsetse and Trypanosomiasis Control Programme in Zambézia, Tete and Sofala provinces. 相似文献
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Tom B. Yarbrough DVM Diplomate ACVS ED Voss DVM Eric J. Herrgesell DVM Diplomate ACVR Michelle Shaw DVM 《Veterinary surgery : VS》1999,28(4):287-291
OBJECTIVE: To report the clinical signs and management of 4 foals with persistent frenulum of the epiglottis. STUDY DESIGN: Case report. ANIMALS: Four newborn foals. METHODS: Foals were admitted with a complaint of oronasal reflux after nursing. Variable systemic signs of aspiration pneumonia were evident. Nasal endoscopy confirmed persistent dorsal displacement of the soft palate. Persistent frenulum of the epiglottis, confirmed by oral endoscopic examination, was transected. RESULTS: After surgery, all foals had the epiglottis positioned normally, dorsal to the soft palate. Clinical signs of oronasal reflux resolved by the second nursing attempt in 3 foals, whereas this was difficult to assess in one foal that was nursed intermittently because of the mares behavior. This foal died 2 days later. The other 3 foals have had normal epiglottic function for 2-4 years. CONCLUSIONS: Persistent frenulum of the epiglottis should be considered in foals with oronasal reflux from birth. With appropriate medical and surgical management the prognosis for resolution should be good. 相似文献
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A 15‐year‐old, spayed, female poodle dog was presented for evaluation of a mass of tissue prolapsed from the vulva. The dog had been hysterectomized when it was 5 years old. A vaginal mass had been removed approximately 10 months before presentation. Haematological and serum biochemistry analyses demonstrated mild leucocytosis and glycaemia. A vaginal smear was predominantly made up of parabasal cells and intermediate cells with no neoplastic cells. Thoracal and abdominal radiographic findings were unremarkable. The ovaries could not be identified using abdominal ultrasonography. A midline exploratory laparotomy identified both ovaries that were surgically excised. The vaginal mass was also removed following an episiotomy procedure. Histopathological examination of the mass demonstrated that it was a neurofibroma. Both ovaries had cystic changes. Four months after the surgery, the owner reported that the dog was clinically normal. To the authors’ knowledge, this is the first reported case of a vaginal neurofibroma after an incomplete ovariohysterectomy in the dog. 相似文献
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Katharina Bollig Andr�� Specht San Shwe Myint Marc Zahn Walter J. Horst 《European journal of plant pathology / European Foundation for Plant Pathology》2013,135(1):81-96
Vascular wilt caused by the soil-borne fungus Verticillium dahliae is a major yield and quality-limiting disease across a broad spectrum of crop plants worldwide. Sulphur-enhanced plant defence mechanisms provide an opportunity to effectively and environmentally safely constrain the wilt disease levels in planta. To evaluate the influence of sulphur nutrition on the protective potential of these mechanisms, two near-isogenic tomato genotypes differing in fungal susceptibility, were treated with low or supra-optimal sulphur supply. Microscopic analysis revealed a significant sulphur-induced decrease in the amount of infected vascular cells in both genotypes. However, plant shoot and severely pathogen-affected root growth did not display this distinct alleviating influence of sulphur nutrition. Rates of leaf photosynthesis were impeded by Verticillium dahliae infection in both genotypes especially under low sulphur nutrition. However, assimilate transport rates in the phloem sap were enhanced by fungal infection more in the resistant genotype and under high sulphur nutrition suggesting a stronger sink for assimilates in infected plant tissues possibly involved in sugar-induced defence. A SYBR Green-based absolute quantitative Real-Time assay using a species-specific primer was developed which sensitively reflected sulphur nutrition-dependent changes in fungal colonization patterns. High sulphur nutrition significantly reduced fungal spread in the stem in both tomato genotypes. Concentrations of selected sulphur-containing metabolites revealed an increase of the major anti-oxidative redox buffer glutathione under high sulphur nutrition in response to fungal colonization. Our study demonstrates the existence of sulphur nutrition-enhanced resistance of tomato against Verticillium dahliae mediated by sulphur-containing defence compounds. 相似文献