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1.
It is unknown whether overlapping or sequential use of nonsteroidal anti‐inflammatory (NSAIDs) results in an increased risk for gastrointestinal (GI) ulceration. The purpose of this pilot study was to evaluate the GI effects of various combinations of an injectable NSAID followed by an oral NSAID, a scenario often employed clinically for management of the pre‐ and post‐operative canine patient. Six healthy Walker hounds received four treatment regimens in a randomized, cross‐over design with a 2 week washout period between each treatment week: carprofen (4 mg kg–1, SQ) followed by placebo (PO, q24 × 4 days); placebo (SQ) followed by deracoxib (3–4 mg kg–1, PO, q24 × 4 days); carprofen (4 mg kg–1, SQ) followed by carprofen (4 mg kg–1, PO, q24 × 4 days); carprofen (4 mg kg–1, SQ) followed by deracoxib (3–4 mg kg–1, PO, q24 ×4 days). Weekly bloodwork (CBC, biochemistry panel, fecal evaluation, fecal occult blood) and daily clinical scoring (TPR, vomiting, diarrhea, appetite) were obtained. GI endoscopy was performed on days –2, 1, 2, 5, and 11 days post treatment of each treatment period and lesions scored using a previously reported 6‐point scale. Data was analyzed using a mixed anova for repeated measures. There were no significant differences in clinical or clinicopathologic data between groups. Within the carprofen‐carprofen and carprofen‐deracoxib groups, lesions worsened by Day 5 (1 day after last oral dose) for the fundic and antral regions (p < 0.05). Fundic, antral and lesser curvature lesions improved by Day 5 in the carprofen‐placebo group and lesser curvature lesions improved in the placebo‐deracoxib group (p < 0.05). No significant within‐group differences were noted for the esophagus, cardia or duodenum. The small number of dogs precludes general conclusions about the safety of sequential NSAID use, but these results suggest that a larger scale study is warranted. 相似文献
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Carvalho RF Campos ML Pino LE Crestana SL Zsögön A Lima JE Benedito VA Peres LE 《Plant methods》2011,7(1):18-14
Background
The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably compare phenotypes. The lack of tomato developmental mutants in a single genetic background prevents the stacking of mutations to facilitate analysis of double and multiple mutants, often required for elucidating developmental pathways.Results
We took advantage of the small size and rapid life cycle of the tomato cultivar Micro-Tom (MT) to create near-isogenic lines (NILs) by introgressing a suite of hormonal and photomorphogenetic mutations (altered sensitivity or endogenous levels of auxin, ethylene, abscisic acid, gibberellin, brassinosteroid, and light response) into this genetic background. To demonstrate the usefulness of this collection, we compared developmental traits between the produced NILs. All expected mutant phenotypes were expressed in the NILs. We also created NILs harboring the wild type alleles for dwarf, self-pruning and uniform fruit, which are mutations characteristic of MT. This amplified both the applications of the mutant collection presented here and of MT as a genetic model system.Conclusions
The community resource presented here is a useful toolkit for plant research, particularly for future studies in plant development, which will require the simultaneous observation of the effect of various hormones, signaling pathways and crosstalk. 相似文献4.
Dimitrios FanourakisSusana M.P. Carvalho Domingos P.F. Almeida Olaf van KootenWouter G. van Doorn Ep Heuvelink 《Postharvest Biology and Technology》2012,64(1):64-73
A constant high relative air humidity (RH) during cultivation can strongly reduce the vase life in some cut rose cultivars. We studied three contrasting cultivars in their tolerance to high RH in order to analyse in detail the water relations during postharvest and better understand this genotypic variation. Plants were grown at moderate (60%) and high (95%) RH, and cut flowers were placed in water immediately after cutting. Flowers of cv. Pink Prophyta grown at high RH did not open throughout vase life, while flower opening of cvs. Frisco and Dream was not affected by preharvest RH. Cultivation at high RH resulted in about 80% shorter vase life in Pink Prophyta, whereas in Dream and Frisco the negative effect was considerably smaller (15 and 9% shorter vase life, respectively). The shorter vase life and reduced flower opening of cut roses grown at high RH was due to a higher rate of transpiration both in the light and dark periods. It was found that the leaves of Pink Prophyta grown at high RH could partly close their stomata upon lowering of the water potential or when flower stalks were fed with abscisic acid, but stomata remained far more open than in leaves grown at moderate RH. The RH during cultivation did not affect stem hydraulic conductivity and its recovery after air emboli induction. Preventing vascular occlusion largely alleviated the high-cultivation-RH effect on vase life and flower opening, showing that the effect of high-cultivation-RH becomes only important if water uptake is limited. 相似文献
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Fran?oise P Sanches Thaise Y Tomokane Vania LR Da Matta Mary Marcondes Carlos EP Corbett Márcia D Laurenti 《Acta veterinaria Scandinavica》2014,56(1)
Background
There are only a few studies reporting the role of nitric oxide metabolites for controlling macrophage intracellular parasitism, and these are controversial. Therefore, the present study aimed to evaluate the expression of inducible nitric oxide synthase (iNOS) in the lymph nodes and spleen of dogs affected by visceral leishmaniasis through immunohistochemistry and to determine its correlation with tissue parasite burden and serum interferon (IFN)-γ levels. Twenty-eight dogs were selected and assigned to one of two groups, symptomatic (n = 18) and asymptomatic (n = 10), according to clinical status and laboratory evaluation. A negative control group (n = 6) from a non-endemic region for visceral leishmaniasis was included as well.Results
Parasite density (amastigotes/mm2) was similar between clinical groups in the lymph nodes (P = 0.2401) and spleen (P = 0.8869). The density of iNOS+ cells was higher in infected dogs compared to controls (P < 0.05), without a significant difference in lymph node (P = 0.3257) and spleen (P = 0.5940) densities between symptomatic and asymptomatic dogs. A positive correlation was found between the number of iNOS+ cells in lymph nodes and interferon-γ levels (r = 0.3776; P = 0.0303), and there was a negative correlation between parasites and iNOS+ cell densities both in lymph nodes (r = −0.5341; P = 0.0034) and spleen (r = −0.4669; P = 0.0329).Conclusion
The negative correlation observed between tissue parasitism and the expression of iNOS may be a reflection of NO acting on the control of parasites. 相似文献6.
Berends IM Graat EA Swart WA Weber MF van de Giessen AW Lam TJ Heuvelink AE van Weering HJ 《Preventive veterinary medicine》2008,87(3-4):301-310
The aim of this study was to determine the herd prevalence of veal and dairy herds and to identify risk factors for VTEC O157 positive veal herds. The study was based on monitoring data from November 1996 through July 2005 of 1051 dairy herds and 930 veal herds. The herd level prevalence (95% CI) was 8.0% (6.4–9.6) for dairy herds and 12.6% (10.5–14.7) for veal herds. Within the population of veal herds, a prevalence of 39.8% (33.9–45.6) was found for pink veal herds (n = 269) and 1.5% (0.7–2.8) for white veal herds (n = 661).Multivariable logistic regression showed that the type of veal (pink vs. white; OR = 21.6; 95% CI: 10.4–45.0), ventilation (mechanical vs. natural; OR = 0.4; 95% CI: 0.2–0.8), time between arrival in the herd and sampling (3–5 months vs. 0–2 months: OR = 2.33; 95% CI: 1.1–5.1, ≥6 months vs. 0–2 months: OR = 4.11; CI: 1.9–8.9), other feed than the 7 most common (yes vs. no; OR = 2.1; 95% CI: 1.2–3.7) and at least one dog present in the stable (yes vs. no; OR = 2.6; 95% CI: 1.5–4.6) were significantly (P < 0.05) associated with the presence of VTEC O157. The large difference in the VTEC O157 prevalences for pink veal and white veal production might have been caused by a very different management of these type of herds. However, this could not be studied with the data collected. 相似文献
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B. Kempen G. B. M. Heuvelink D. J. Brus J. J. Stoorvogel 《European Journal of Soil Science》2010,61(3):333-347
This paper compares three models that use soil type information from point observations and a soil map to map the topsoil organic matter content for the province of Drenthe in the Netherlands. The models differ in how the information on soil type is obtained: model 1 uses soil type as depicted on the soil map for calibration and prediction; model 2 uses soil type as observed in the field for calibration and soil type as depicted on the map for prediction; and model 3 uses observed soil type for calibration and a pedometric soil map with quantified uncertainty for prediction. Calibration of the trend on observed soil type resulted in a much stronger predictive relationship between soil organic matter content and soil type than calibration on mapped soil type. Validation with an independent probability sample showed that model 3 out‐performed models 1 and 2 in terms of the mean squared error. However, model 3 over‐estimated the prediction error variance and so was too pessimistic about prediction accuracy. Model 2 performed the worst: it had the largest mean squared error and the prediction error variance was strongly under‐estimated. Thus validation confirmed that calibration on observed soil type is only valid when the uncertainty about soil type at prediction sites is explicitly accounted for by the model. We conclude that whenever information about the uncertainty of the soil map is available and both soil property and soil type are observed at sampling sites, model 3 can be an improvement over the conventional model 1. 相似文献
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LJ Lanzerotti K Rinnert G Dehmel FO Gliem EP Krider MA Uman J Bach 《Science (New York, N.Y.)》1996,272(5263):858-860
During the Galileo probe's descent through Jupiter's atmosphere, under the ionosphere, the lightning and radio emission detector measured radio frequency signals at levels significantly above the probe's electromagnetic noise. The signal strengths at 3 and 15 kilohertz were relatively large at the beginning of the descent, decreased with depth to a pressure level of about 5 bars, and then increased slowly until the end of the mission. The 15-kilohertz signals show arrival direction anisotropies. Measurements of radio frequency wave forms show that the probe passed through an atmospheric region that did not support lightning within at least 100 kilometers and more likely a few thousand kilometers of the descent trajectory. The apparent opacity of the jovian atmosphere increases sharply at pressures greater than about 4 bars. 相似文献
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In 2004 the Food and Consumer Product Safety Authority (VWA) investigated the hygiene and hygiene facilities on 125 children's farms. In general, both the level of hygiene and the availability of hygiene facilities were good. A previous investigation, carried out in 2002, had highlighted a number of points for improvement, such as the need to improve hand-washing facilities. While the situation was better in 2004, it still did not meet the standard laid down by the VWA. The VWA aspires to achieve 100% implementation of the requirement that children's farms have a Code for Hygiene and an information board. Investigation of faecal samples collected in 2002 showed the presence of STEC O157 on 13 (10.2%) of the visited farms, Salmonella spp. on 19 (14.5%) and Campylobacter spp. on 74 (56.6%). These results show that there is a real risk of becoming infected with a zoonotic pathogen when visiting a children's farm. This emphasizes the importance of strict adherence to hygiene measures by workers and visitors on children's farms in The Netherlands. 相似文献
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GHM Araujo AN Rocha Filho EP Lopes CF Moya MA Alvarenga 《Reproduction in domestic animals》2009,44(3):380-383
The effects of a low dose of equine purified FSH (eFSH) on incidence of multiple ovulations and embryo recovery rate in mares were studied. During the physiological breeding season in Brazil (19°45′45′S), 14 Mangalarga Marchador donor mares were used in a crossover study and another 25 mares of the same breed, between 3 years and 12 years of age were used as recipients for the embryo transfers. Donors were monitored during two consecutive oestrus cycles, an untreated control cycle followed by a treated cycle, when eFSH was administered. In both cycles, after an embryo collection attempt on day 8 post‐ovulation all mares received 7.5 mg dinoprost and had their two largest follicles tracked daily by ultrasonography until the period of ovulation. Mares were inseminated every 48 h with extended fresh semen from a single stallion after the identification of a 35‐mm follicle until the period of ovulation. Ovulations were induced by intravenous administration of 2.500 IU of human chorionic gonadotropin, upon detection of a 35‐ to 40‐mm follicle. In the treated cycle, 5 mg eFSH was given intramuscularly once a day, from day 8 post previous ovulation until at least one follicle reached 35 mm in diameter. Embryo flushes were performed on day 8 of dioestrus (day 0 = ovulation). Treatment with eFSH resulted in higher (p < 0.05) ovulation rate and incidence of multiple ovulations compared to the control (1.6 vs 1.0 and 50% vs 0%, respectively – one mare had triple ovulation). However, embryo recovery rates in the control and treated cycles were similar (0.8 and 1.0, respectively; p > 0.05). Pregnancy rates in the recipient mares following embryo transfer were similar for the control and eFSH cycles (11/11 and 10/14, respectively). Additional studies are necessary in order to develop a low‐dose protocol for the use of eFSH that brings a more consistent contribution to the efficiency of commercial equine embryo transfer programs. 相似文献