Pronuclear Embryo Yield in Canindé and Saanen Goats for DNA Microinjection     

RR Moura  ES Lopes‐Junior  DIA Teixeira  IA Serova  LE Andreeva  LM Melo  VJF Freitas 《Reproduction in domestic animals》2010,45(5):e101-e106

The objective of this study was to examine the effect of donor breed on pronuclear‐stage embryo yield to be used for DNA microinjection in a transgenesis goat program. Twelve Canindé and twelve Saanen goats were heat synchronized using a progestagen‐cloprostenol treatment. Forty‐eight hours before the sponge removal, superovulation was induced with a total administration of 4.4 mg/kg bodyweight NIH‐FSH‐P1, given twice daily in decreasing doses over 3 days. In addition, goats received 100 μg of GnRH and they were hand‐mated at 36 and 48 h after progestagen removal. Embryo recovery was performed by oviduct flushing at 72 h after sponge removal. Embryos were microinjected with a DNA construct and noticeable swelling of the nuclei was the criterion for successful microinjection. The total diameter, cytoplasm diameter, zona pellucida thickness and pronuclei diameter were measured for each microinjected embryo. A higher (p < 0.05) percentage of fertilized ova was observed in Canindé (89.9%) than Saanen (36.2%) goats. In addition, Canindé donors produced a higher percentage of pronuclear embryos when compared with Saanen: 72.5% vs 20.6% (p < 0.05), respectively. Successful microinjection was verified in 96.7% and 73.3% of times in Canindé and Saanen embryos, respectively (p < 0.05). Significant differences were observed for all morphometric parameters except for cytoplasm diameter. In conclusion, under our study experimental conditions, Canindé were more efficient than Saanen goats concerning the pronuclear embryo yield and manipulation. The use of Canindé goats in transgenesis programs could be increase the interest in their breeding and could be contribute to saving them from extinction.  相似文献   

Stability of emulsifiable concentrate pesticide formulations in liquid fertilizers     

Sarina Saltzman  Nelly Brates 《Pest management science》1990,28(2):181-195

The stability of some diluted emulsifiable concentrate formulations in clear liquid fertilizers, as affected by the nature and concentration of the salt solutions, was checked by a static test. The time-induced changes in the concentration of the dispersed phase were estimated by visual observations and turbidity measurements. For each formulation a specific relationship between the electrical conductivity of all the fertilizer solutions and the emulsion stability was found. This was valid for a pH range between 4.6–6.3; in alkaline conditions the emulsion stability was relatively lower. A critical electrical conductivity range, above which irreversible destabilization occurred, was observed for each formulation: 100-120 dS m^?1 for fenamiphos, 60-70 dS m^?1 for metolachlor, 30-50 dS m^?1 for chlorpyrifos, and about 45 dS m^?1 for S-ethyl dipropylthiocarbamate (EPTC). The correlation observed between the emulsion stability and the electrical conductivity could be used in a simple and rapid qualitative test to estimate the physical compatibility between emulsifiable concentrate formulations and liquid fertilizers.  相似文献   

A rapid bioassay for the detection of photosynthesis inhibitors in water     

Sarina Saltzman  Bruria Heuer 《Pest management science》1985,16(5):457-462

A modified leaf disc buoyancy procedure for the detection of photosynthesis-inhibiting residues in water is described. The modifications proposed, mainly the presence of sodium hydrogen carbonate in the infiltration solution, increased the sensitivity of the method and reduced the time required. The substituted urea and 1,3,5-triazine herbicides diuron, linuron, monuron, atrazine, ametryn and atraton were detected below 0.7 mg litre^?1 using cucumber (Cucumis sativus L., cv. ‘Dalia’) leaf discs. A concentration as low as 0.09 mg diuron litre^?1 could be detected. Although bean (Phaseolus vulgaris L., cv. ‘Bulgarian’) leaf tissue was less sensitive in this bioassay than cucumber, 0.3 mg diuron litre^?1 could still be detected. The test, being very rapid (less than 30 min per determination) and relatively sensitive, could be used for the detection of photosynthesis inhibitors in recycled water used for irrigation.  相似文献   

20th-century industrial black carbon emissions altered Arctic climate forcing   总被引：1，自引：0，他引：1  

McConnell JR  Edwards R  Kok GL  Flanner MG  Zender CS  Saltzman ES  Banta JR  Pasteris DR  Carter MM  Kahl JD 《Science (New York, N.Y.)》2007,317(5843):1381-1384

Black carbon (BC) from biomass and fossil fuel combustion alters chemical and physical properties of the atmosphere and snow albedo, yet little is known about its emission or deposition histories. Measurements of BC, vanillic acid, and non-sea-salt sulfur in ice cores indicate that sources and concentrations of BC in Greenland precipitation varied greatly since 1788 as a result of boreal forest fires and industrial activities. Beginning about 1850, industrial emissions resulted in a sevenfold increase in ice-core BC concentrations, with most change occurring in winter. BC concentrations after about 1951 were lower but increasing. At its maximum from 1906 to 1910, estimated surface climate forcing in early summer from BC in Arctic snow was about 3 watts per square meter, which is eight times the typical preindustrial forcing value.  相似文献   

Unconditional quantum teleportation   总被引：5，自引：0，他引：5  

A Furusawa  JL Sorensen  SL Braunstein  CA Fuchs  HJ Kimble  ES Polzik 《Science (New York, N.Y.)》1998,282(5389):706-709

Quantum teleportation of optical coherent states was demonstrated experimentally using squeezed-state entanglement. The quantum nature of the achieved teleportation was verified by the experimentally determined fidelity Fexp = 0.58 +/- 0.02, which describes the match between input and output states. A fidelity greater than 0.5 is not possible for coherent states without the use of entanglement. This is the first realization of unconditional quantum teleportation where every state entering the device is actually teleported.  相似文献   

Long‐Term Outcome after Arthroscopic Debridement of Distal Phalanx Extensor Process Fragmentation in 13 Horses     

OLIVER M. CROWE  BVSc Cert ES    RICHARD J. HEPBURN  BVSc Cert EM Diplomate ACVIM    SVEND E. KOLD  DVM PhD    ROGER K. SMITH  MA Vet MB PhD DEO Diplomate ECVS 《Veterinary surgery : VS》2010,39(1):107-114

Objective— To report long-term outcome after arthroscopic removal of fragmentation of the extensor process of the distal phalanx in horses.
Study Design— Case series.
Animals— Adult horses (n=13).
Methods— Medical records (2003–2004) of horses that had arthroscopic debridement of fragmentation of the extensor process of the distal phalanx were reviewed. Inclusion criteria included: lameness localized to the foot, fragmentation of the extensor process of the distal phalanx debrided arthroscopically, and a follow-up period of ≥4 years.
Results— Of the 13 horses, lameness was resolved in 11 (85%) initially but distal interphalangeal joint pain recurred in 2 (15%) within 1 year of surgery. Three (23%) other horses were retired because of lameness at other sites during the follow-up period resulting in 46% (6/13) being not lame and in full work after 4 years. Substantial changes were identified at surgery in the contralateral joint of 7 horses, even when fragments were only present unilaterally.
Conclusions— Arthroscopic debridement of fragmentation of the extensor process of the forelimb distal phalanx has a good short-term prognosis for resolution of lameness and return to work but a more guarded prognosis for long-term soundness.
Clinical Relevance— These results allow for more accurate prognostication preoperatively and may support early debridement of fragmentation of the extensor process of the distal phalanx.  相似文献   

Wiley Award Address: analysis of air contaminants.     

B E Saltzman 《Journal of the Association of Official Analytical Chemists》1979,62(2):214-225

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Effects of an extreme flash flood on the native fish assemblages across a Mediterranean catchment     

A. M. PIRES  M. F. MAGALHÃES  L. MOREIRA DA COSTA  M. J. ALVES  & M. M. COELHO 《Fisheries Management and Ecology》2008,15(1):49-58

Abstract  The effects of an extreme flash flood on the native fish assemblages across a Mediterranean catchment in Southwest Portugal were investigated. The fish community structure and habitat were surveyed at 10 sites pre- and post-flood. Overall species richness and rank abundances changed little after the flood, despite there being a consistent decline in the abundance of small cyprinids. Pre- and post-flood habitat correlates of abundance changed for at least some species, and variability in assemblage structure was associated with flood, depth and pH. These results indicate that extreme floods occurring early in the wet season may have little disruptive effects on the overall structure of native fish assemblages in Mediterranean streams, although may at least partially influence population dynamics for some species. Habitat quality and complexity are likely to play a role in mediating species responses to flooding.  相似文献   

Abstracts of papers presented at a meeting of the weed science society of israel on possibilities of reducing herbicide use     

Stuart Z. Cohen  A. Eisenberg  U. Mingelgrin  Z. Gerstl  Sarina Saltzman  M. Davis  Z. Paster  H. Haas  Y. Kleifeld  U. Rosenberg  J. C. Caseley  B. Rubin  H. H. Lebaron  M. Horowitz  Ziva Amsellem  A. Sharon  J. Gressel  H. Aviram  E. Shlevin 《Phytoparasitica》1991,19(4):319-330

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Evaluation of cardiorespiratory and biochemical effects of ketamine‐propofol and guaifenesin‐ketamine‐xylazine anesthesia in donkeys (Equus asinus)     

Cássia M Molinaro Coelho  Juan C Duque Moreno  Daniel da S Goulart  Leandro B Caetano  Lorena K Soares  Gustavo H Coutinho  Geraldo ES Alves  Luiz Antonio F da Silva 《Veterinary anaesthesia and analgesia》2014,41(6):602-612

ObjectivesTo evaluate the cardiorespiratory and biochemical effects of ketamine-propofol (KP) or guaifenesin-ketamine-xylazine (GKX) anesthesia in donkeys.Study designProspective crossover trial.AnimalsEight healthy, standard donkeys, aged 10 ± 5 years and weighing 153 ± 23 kg.MethodsDonkeys were premedicated with 1.0 mg kg^?1 of xylazine (IV) in both treatments. Eight donkeys were administered ketamine (1.5 mg kg^?1) and propofol (0.5 mg kg^?1) for induction, and anesthesia was maintained by constant rate infusion (CRI) of ketamine (0.05 mg kg^?1 minute^?1) and propofol (0.15 mg kg^?1 minute^?1) in the KP treatment. After 10 days, diazepam (0.05 mg kg^?1) and ketamine (2.2 mg kg^?1) were administered for induction, and anesthesia was maintained by a CRI (2.0 mL kg^?1 hour^?1) of ketamine (2.0 mg mL^?1), xylazine (0.5 mg mL^?1) and guaifenesin (50 mg mL^?1) solution. Quality of anesthesia was assessed along with cardiorespiratory and biochemical measurements.ResultsAnesthetic induction took longer in GKX than in KP. The induction was considered good in 7/8 with KP and in 6/8 in GKX. Anesthetic recovery was classified as good in 7/8 animals in both treatments. Xylazine administration decreased heart rate (HR) in both treatments, but in KP the HR increased and was higher than GKX throughout the anesthetic period. Respiratory rate was higher in GKX than in KP. PaO₂ decreased significantly in both groups during the anesthetic period. Glucose concentrations [GLU] increased and rectal temperature and PCV decreased in both treatments. Arterial lactate [LAC] increased at recovery compared with all time points in KP. [GLU] and calcium were higher in GKX than in KP at recovery.Conclusion and clinical relevanceThese protocols induced significant hypoxemia but no other cardiorespiratory or metabolic changes. These protocols could be used to maintain anesthesia in donkeys, however, they were not tested in animals undergoing surgery.  相似文献