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Filippi Patrick Whelan Brett M. Vervoort R. Willem Bishop Thomas F. A. 《Precision Agriculture》2022,23(2):578-601
Precision Agriculture - There is considerable interest and value in identifying the gap between crop yields that have actually been achieved, and yields that could have potentially been achieved. A... 相似文献
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Gu Y Filippi MD Cancelas JA Siefring JE Williams EP Jasti AC Harris CE Lee AW Prabhakar R Atkinson SJ Kwiatkowski DJ Williams DA 《Science (New York, N.Y.)》2003,302(5644):445-449
The Rho guanosine triphosphatases (GTPases) Rac1 and Rac2 are critical signaling regulators in mammalian cells. The deletion of both Rac1 and Rac2 murine alleles leads to a massive egress of hematopoietic stem/progenitor cells (HSC/Ps) into the blood from the marrow, whereas Rac1-/- but not Rac2-/- HSC/Ps fail to engraft in the bone marrow of irradiated recipient mice. In contrast, Rac2, but not Rac1, regulates superoxide production and directed migration in neutrophils, and in each cell type, the two GTPases play distinct roles in actin organization, cell survival, and proliferation. Thus, Rac1 and Rac2 regulate unique aspects of hematopoietic development and function. 相似文献
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C Vitu P Russo P Filippi R Vigne G Querat A Giauffret 《Comparative immunology, microbiology and infectious diseases》1982,5(4)
An indirect microELISA test was performed for detection of maedi-visna antibodies in ovine and caprine species. The antigen consisted in viral particles, highly purified by successive ultracentrifugations.By comparative testing of 934 sera in ELISA and gel immunodiffusion, we found a good correlation between these two teṡts, and moreover, ELISA revealed another 11.3% of positive samples. The precocity of this ELISA was shown by experimental infection of sheep with different strains of maedi-visna: positive sera were detected 7 weeks post-infection, instead 4–5 months with gel immunodiffusion. The complement fixation test was compared with gel immunodiffusion and was found the less sensitive.This ELISA test appeared to be satisfactory, and may be used for early diagnosis of maedi-visna infection. 相似文献
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Filippi JJ Lanfranchi DA Prado S Baldovini N Meierhenrich UJ 《Journal of agricultural and food chemistry》2006,54(17):6308-6313
The essential oil of Achillea ligustica from Corsica was investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 82 compounds representing 94.0% of the oil were tentatively identified. The main constituents were the camphane derivatives, representing >30% (camphor, 21.3%; borneol, 6.2%; bornyl acetate, 3.5%) of the whole oil, and santolina alcohol (19.3%). The enantiomeric distribution of 8 chiral constituents was determined by GC-MS using two enantioselective stationary phases (DIME-beta-CD and Lipodex-E). Racemic santolina alcohol, required for optimization of the enantioselective GC conditions, was prepared by an original two-step synthesis from 2,5-dimethylhexa-2,4-diene. The whole essential oil was tested for its antibacterial activity against a wide range of bacteria using a paper disk method. The results show a promising activity against Streptomyces species. 相似文献
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Trevisi P Melchior D Mazzoni M Casini L De Filippi S Minieri L Lalatta-Costerbosa G Bosi P 《Journal of animal science》2009,87(1):148-156
We tested the effect of Trp addition to a standard weaning diet and oral challenge with enterotoxigenic Escherichia coli K88 (ETEC) on growth and health of piglets susceptible or nonsusceptible to the intestinal adhesion of ETEC. Sixty-four pigs weaned at 21 d of age were divided into 3 groups based on their ancestry and BW: a control group of 8 pigs fed a basal diet (B), the first challenged group of 28 pigs fed B diet (BCh), and the second challenged group of 28 pigs fed a diet with Trp (TrpCh). The Trp diet was produced by the addition of 1 g of l-Trp/kg to the basal diet. On d 5, pigs were orally challenged with 1.5 mL suspension containing 10(10) cfu ETEC/mL or placebo, and killed on d 9 or 23. Based on in vitro villus adhesion assay, the pigs (except the B group) were classified as susceptible (s(+)) or nonsusceptible (s(-)) to the intestinal ETEC adhesion. Thus, after the challenge, treatments were B, BChs(-), BChs(+), TrpChs(-), and TrpChs(+). Pigs susceptible to ETEC were 50.0% in the BChs(+) group (3 pigs lost included) and 46.4% in the TrpChs (+) group (1 pig lost included). During the first 4 d after challenge, the challenge reduced ADG (P < 0.05), and this reduction was greater in susceptible pigs (P < 0.05) than nonsusceptible ones. Tryptophan increased ADG and feed intake in susceptible pigs (P < 0.05) from challenge to d 4, but not thereafter. Tryptophan supplementation did not improve the fecal consistency and did not reduce the number of pigs positive for ETEC in feces on d 4 after the challenge. The K88-specific immunoglobulin A activity in blood serum tended to be greater in challenged pigs (P = 0.102) and was not affected by the addition of Trp. Villous height was affected by the addition of Trp and challenge in different ways, depending on the site of small intestine. The need to consider the phenotype for the adhesion of the ETEC in studies with different supply of Trp was clearly evident. When compared with practical weaning standard diets, Trp supplementation allowed susceptible pigs to partially compensate for the effects of ETEC challenge by increasing feed intake and maintaining an adequate BW growth. This is of practical importance for the formulation of diets for pigs selected for lean growth because of the presence of an association between this trait and the susceptibility to the intestinal adhesion of ETEC. 相似文献
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P Russo C Vitu R Vigne P Filippi A Giauffret 《Comparative immunology, microbiology and infectious diseases》1988,11(1):35-41
In order to study pathogenicity of sheep lentiviruses, to obtain monospecific sera and to perfect ELISA, 3 experiments with different strains were carried out for 4 yr. In expt 1, one clone only of a French maedi-visna strain (564-79) elicits a clear seroconversion in inoculated sheep. In expt 2, K1514 is more immunogenic than K796 and PPV: intratracheal route seems more efficient than intracerebral route. Sheep infected by ts mutants (expt 3) are early positive as wild strain K796. Nevertheless, the level of positivity is less important than for the parental strain, suggesting that the defect of the ts mutants is not limiting their replication in vivo. An important result is the lack of clinical signs and anatomical and histopathological lesions, in spite of frequent isolations of virus from buffy coat cells. These results suggest that: different enhancing factors have to be taken in account in the apparition of clinical signs; all the clones are not infectious; viral infection might be effective with several types of virions. 相似文献
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Marco Vuerich Elisa Petrussa Antonio Filippi Stéphanie Cluzet Josep Valls Fonayet Angela Sepulcri Barbara Piani Paolo Ermacora Enrico Braidot 《Pest management science》2023,79(7):2503-2516
BACKGROUND
In recent years, biofungicides have drawn increasing interest in vineyards for a more sustainable integrated and copper-limited pest management. Among alternatives, botanicals could represent valuable tools, being rich sources of biologically active compounds. Conversely to the well-known antioxidant and biological properties in relation to health benefits, investigation on bioactivity of hot pungent Capsicum sp. products against fungal phytopathogens in vineyards is still scarce. Therefore, the present study aimed at exploring the biologically active compounds profile of a chili pepper (Capsicum chinense Jacq.) pod extract and its antimicrobial properties against some of the major fungal and Oomycetes pathogens of grapevine, including Botrytis cinerea Pers., Guignardia bidwellii (Ellis) Viala & Ravaz and Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni.RESULTS
The ethyl acetate-extracted oleoresin from the most pungent varieties was rich in capsaicinoids and polyphenols (371.09 and 268.5 μg mg−1 dry weight, respectively). Capsaicin and dihydrocapsaicin, hydroxycinnamic and hydroxybenzoic acids and quercetin derivatives were the most abundant, while carotenoids represented only a minor fraction. The oleoresin was efficient to inhibit all three pathogenic fungi and ED50 values were determined, evidencing that G. bidwellii was the more sensitive (0.233 ± 0.034 mg mL−1).CONCLUSION
The results suggested a potentiality of chili pepper extract for the control of some important grapevine pathogens, their possible application being helpful for the recommended limitation in extensive use of copper in vineyard. The complex mixture of high amounts of capsaicinoids, associated to specific phenolic acids and other minor bioactive components might contribute to the observed antimicrobial action of chili pepper extract. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献9.
Bertelli AA Migliori M Filippi C Gagliano N Donetti E Panichi V Scalori V Colombo R Mannari C Tillement JP Giovannini L 《Journal of agricultural and food chemistry》2005,53(17):6924-6929
Ochratoxin A (OTA), is a nephrotoxic mycotoxin present in wine, which is nephrotoxic in humans. Our working hypothesis is that natural substances in wine may counteract OTA toxicity. Thirty-six rats were randomized to OTA dissolved in saline, red wine, or 13.5% ethanol or to OTA-free wine, ethanol, or saline. OTA (289 microg/kg of body weight/48 h) was administered by gastric gavage for 2 weeks. Serum creatinine, tubular enzymuria, renal lipohydroperoxides (LOOH), reduced (GSH) and oxidized (GSSG) glutathione, and renal superoxide dismutase activity (SOD) were determined in renal tissue. OTA alone produced significant increases in renal lipoperoxides and significant decreases in SOD and GSH/GSSG ratio. In red wine or ethanol, OTA was less nephrotoxic, reducing oxidative damage as revealed by LOOH. In OTA-wine and OTA-ethanol groups, SOD activity was higher than in the OTA-treated one, suggesting that both ethanol and nonalcoholic fractions may preserve antioxidant reserve. GSH/GSSG ratio was significantly preserved only in the OTA-wine group and not in OTA-ethanol. Red wine may exert a protective effect against OTA nephrotoxicity by limiting oxidative damage. The ostensible protection afforded by ethanol deserves further investigation. 相似文献
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Response of photosynthesis and chlorophyll a fluorescence in leaf scald‐infected rice under influence of rhizobacteria and silicon fertilizer 
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下载免费PDF全文 A. C. S. O. Bueno G. L. S. Castro D. D. Silva Junior H. A. Pinheiro M. C. C. Filippi G. B. Silva 《Plant pathology》2017,66(9):1487-1495
Leaf scald caused by Monographella albescens reduces the photosynthetic area, causing yield losses in rice. This study investigated the efficacy of the rhizobacteria Burkholderia pyrrocinia (BRM‐32113) and Pseudomonas fluorescens (BRM‐32111), combined with silicon (Si) fertilization, to reduce lesion size and the area under the disease progress curve (AUDPC), as well as to minimize the negative effects on gas exchange, chlorophyll a fluorescence, chlorophyll content and the activity of oxidative stress enzymes. The experiment used a completely randomized design with four replications and seven treatments. Compared with plants only fertilized with Si, plants fertilized with Si and treated with BRM‐32113 showed reductions of 22% in scald lesion expansion and 37% in AUDPC, a 27% increase in the rate of CO2 assimilation (A), a 33% decrease in the internal CO2 concentration (Ci), and a 40% increase in ascorbate peroxidase activity. It was therefore concluded that the combination of BRM‐32113 with Si fertilization reduces the severity of leaf scald, protecting the photosynthetic apparatus, thus representing a sustainable method of reducing the loss of income caused by leaf scald in rice. 相似文献