SUMMARY An aqueous suspension of air-dried, hammer-milled leaf of Terminalla oblongata (yellow-wood) was administered to sheep by gavage, as a single dose of 5 to 20 g (dry weight)/kg body weight. Doses of 15 g/kg, or more, caused depression, Inappetence, abdominal pain and reduced ruminal movements within 24 to 48 h and some sheep also showed dyspnoea, oplsthotonus and champing of the Jaws. Haematology and blood gas and acid-base measurements were unaffected. In sheep given a dose of 12.5 g/kg, or more, plasma osmolality, aspartate aminotransferase activity and potassium and bilirubin concentrations Increased while plasma total protein markedly decreased and plasma sodium concentration and alkaline phosphatase activity remained normal. Most sheep were necropsled 48 h after dosing. The liver showed zonal hepatocellular necrosis, the pattern of which varied with the dose given. No renal lesions were observed, although one sheep given a very high dose became azotaemic and hyperkalaemic. Hydrothorax, hydroperlcardlum and ascltes developed In sheep given doses of 15 or 20 g/kg. 相似文献
The development of the parotid gland was examined in 36 bovine embryos and foetuses with a crown-rump-length (CRL) from 28 up to 1000 mm by light, transmission electron microscopical and actin-immunohistochemical methods. The anlage of the parotid gland in an embryo with 28 mm CRL can be found at the lateral angle of the primitive oral cavity as a local thickening of the epithelium. During the second month, the differentiation of primary ducts and endbuds starts and a lumen develops in the primary ducts. At the end of the second month a lumen appears in the terminal endbuds. In the immature endpiece cells first secretory granules can be seen from a CRL of 240 mm. In the third month differentiation between intra- and inter-lobular ducts is possible. Immature myoepithelial cells present as a basal layer of flattened cells between the epithelial cells and the basement membrane at the end of the second month. During further development they increase in number, become more flattened and form long cellular processes. At the end of the fourth month isolated actin filament bundles are formed, which were also detected by an antibody against smooth muscle actin. The actin filaments condense continuously until they fill the cell processes completely at the end of foetal development. 相似文献
Epidemics of Peronospora parasitica are strongly affected by temperature and air moisture, and the interaction of these factors. Because a significant percentage
of radish plants are grown in greenhouses, it may be possible to influence epidemics by altering the greenhouse climate. The
objective of this study was to test the hypothesis that epidemics of P. parasitica can be modelled by the effects of air temperature and moisture in the greenhouse. Such a model could then be used to analyse
greenhouse climate control strategies with regard to managing downy mildew. Five radish crops were grown under greenhouse
conditions with set-points for heating and ventilation intended to obtain favourable conditions for disease development during
the first part of the growing cycle. Subsequent to this first phase, unfavourable conditions were set until harvest. Disease
incidence was measured once a week until the radishes reached marketable size. In addition, experiments were carried out in
growth chambers in which inoculated plants were subjected to air temperatures between 8 and 27°C, and disease incidence and
sporulation intensity were measured. Data from these two experiments were then used to estimate model parameters. In this
model, the interactions of air temperature (T) and water vapour saturation deficit (SD) were adequately described by a multiplicative
relationship. The simulated epidemics by the fitted model were highly correlated with the observed epidemics (r = 0.91, R2 = 0.83, n = 29). Parameter estimates indicated that T of ca. 20°C and SD < 0.03 hPa resulted in the highest rates of disease development
and that the rate was zero when SD > 2.0 hPa. Both experimental data and simulations showed that epidemics of P. parasitica can be effectively controlled by managing the greenhouse climate. 相似文献
Since the mid-1970's new types of forest damage (of unknown origin) were observed in West Germany. Nonspecific foliage losses and foliar discolorations are the most common symptoms. This resulted in a comprehensive research program being initiated in the fall of 1983 to investigate the nutritional status of Norway spruce (Picea abies (L.) Karst.) stands in southwest Germany, growing on a variety of sites. Forests at these sites revealed light to moderate damage or acute site-and species-specific nutritional disturbances involving Mg, K, Ca, Mn, and Zn. Historical comparisons of needle-analysis data showed a dramatic change of the nutrient supply at many sites over the last one or two decades.
As a working hypothesis, the adverse impacts of air pollutants (e.g. photo-oxidants) and/or acidic atmospheric precipitation are seen as inciting factors causing increased leaching of nutrient elements. The tree's potential to compensate for the nutrient losses by increased uptake are limited by the often poor nutrient supply for forest soils. Accelerated soil acidification increase nutrient leaching from the soil, probably enhancing decline. Over a short period, fertilization led to a decrease or to the disappearance of the symptoms, and to the improvement in the nutrient composition of the foliage.
Microscopy investigation of Norway-spruce needles indicated the regeneration potential on a histological level for moderate yellowed needles after fertilization. Furthermore, needles of nutrient-deficient-trees reveal typical tissue damages different to those caused by SO2 or O3.
Acid mist increased the leaching of K, Ca, Mg, Mn, and Zn from the foliage. Even when nutrients are being leached from the foliage, nutrient uptake canbe increased by improving the nutrient supply in the soil.
Preliminary studies on the effects of damages caused by ozone (O3) on ponderosa pine suggest imbalance in micronutrient concentrations of foliage. 相似文献
The expression of growth factors was evaluated immunohistochemically in normal and cystic ovaries of sows. The immunohistochemically stained area (IHCSA) was quantified by image analysis to analyse the expression of these proteins in the follicular wall of secondary, tertiary and cystic follicles. IGF‐I immunoreactivity was strong in the granulosa cell layer (GC), moderate in the theca interna (TI) and mild in the theca externa (TE) of the normal follicles. There was severe reduction of the labelling to IGF‐I in the GC of the follicular and luteinized cysts. In the normal follicles, the reactivity for IGF‐II was very similar to pattern noted in IGF‐I. There was reduction of the IHCSAs in the GC of the follicular and luteinized cysts, but the decrease was not significant. The staining of the IGF‐II in the TI and TE of the cysts was increased, in comparison with normal follicles. The IHCSAs for VEGF were higher in the GC and TE of the normal follicles in contrast to TI, but this difference was noted only in the tertiary follicle. The VEGF reactivity increased in the GC of the cysts, in relation to normal follicles. The results of the current study show that the formation of ovarian cysts in sows is associated with alterations in the immunohistochemical expression of some growth factors. 相似文献