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This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs.  相似文献   
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A 7-year-old plains zebra (Equus quagga) mare was presented to the Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Germany as an emergency. The owner reported that the mare was unable to stand after a suspected traumatic episode at the home yard. The mare was transported in lateral recumbency in a trailer. Clinical examination revealed tachycardia (up to 102 beats/min), tachypnoea (28 breaths/min), mild fever (38.7°C) and clinical dehydration with elastic skin and dry and red mucous membranes. Neurological examination showed paraplegia with complete loss of nociception of the mid-thoracic region and caudal to it. In contrast, examination of the thoracic limbs revealed marked extensor rigidity and hyperaesthesia resulting in an enhanced acute response to minimal touch of the neck and shoulder region. Lateral radiographs of the thoracic vertebral column led to the diagnosis of a complete fracture of the vertebral body of the 7th thoracic (T) vertebra with suspected traumatic compression of the spinal cord. Post-mortem examination confirmed the diagnosis. Gross pathology revealed a complete comminuted fracture of T7 with marked compression of the spinal cord. This is the first report describing the clinical, radiological and pathological findings of a fracture of a thoracic vertebra leading to a Schiff-Sherrington syndrome in a zebra (Equus quagga).  相似文献   
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The development of a new combined vaccine against cat flu, panleukopenia and rabies should lead to a simplification of the vaccination calendar in small animal practice. In order to judge the suitability of the new vaccine, its efficacy, local tolerability, and safety were evaluated. All vaccinated cats developed high antibody titres against herpes-, calici-, panleukopenia- and rabies virus that persisted well during the trial of 18 months. In comparison to other vaccines containing a smaller number of antigens the suitability of the vaccine on trial could be shown.  相似文献   
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R. Franke 《Plant Breeding》1991,107(4):303-308
One hundred years after the creation of the first true triticale crosses of Rimpau , several recent cultivars of triticale and rye have been crossed with two of them. Recombinants of the awned type of Rimpau 's triticale (10) with ‘Grado’, ‘Lasko’ and ‘Otello’ exhibited a prolonged vegetative period, a shorter straw, an improved lodging resistance, good yielding capacity and higher disease resistance as compared to ‘Lasko’.  相似文献   
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The sustainability of agricultural production is linked to the environment from which it draws its resources. Potato production in the Sandveld in the South African Western Cape occurs in the Cape Floral Kingdom: a vulnerable and globally significant biodiversity hotspot. A scientific approach defining and monitoring sustainability criteria and indicators is required to improve the sustainability of potato production in such a sensitive area. In this paper we propose principles related to the ecological impact of potato production (nature preservation, water preservation and the minimization of chemical and carbon-dioxide emissions) and their derived criteria related to land clearing, irrigation, emissions, and others. Next we defined calculable and measurable indicators of the efficiency with which resources are used, such as proportion of land cleared, water use by the crop, amount of biocides used, the embodied energy of biocides, and the energy needed for farming operations versus the potato yields obtained. In-depth interviews were held with 14 farmers representing 20% of the total potato production area to obtain the current values of these indicators. These were compared to model outcomes of two main sustainability indicators: land and water use efficiency. The land use efficiency varied least between growers, from (36 to 58 Mg (tonnes) ha−1), water use efficiency returned values between 3 and 9 g potato l−1 water, while chemical fertilizer phosphorus use efficiency varied most at 98 and 995 g potato g−1 P applied. Model outcomes confirmed some of the trends revealed by the survey, e.g. growing potatoes in winter and growing them with less than optimal water offers possibilities to double water use efficiency. Ways to derive indicator threshold norms are proposed based on the knowledge of physical and biological processes determining resource availability, the observed variation among farmers and the model outcomes. Knowing indicator values, their range and the means to improve resource use efficiency will aid in establishing sustainability norms by providing a quantitative approach to any environmental certification scheme that wishes to licence the delivery of potatoes from the Sandveld.  相似文献   
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