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排序方式: 共有45条查询结果,搜索用时 171 毫秒
1.
Lectin is released from soybean seeds during water uptake. Hemagglutination activity data show that the lectin is a preferential release product within the first 8 hours of hydration. A qualitative filter-paper assay for detection of lectin released by single seeds is used to show that the release phenomenon is independent of seed viability and insensitive to azide. 相似文献
2.
Analysis of all available observations of faint objects near Saturn during the 1966 passage of the earth through the plane of Saturn's rings suggests the existence of at least one previously undiscovered satellite of Saturn. The data support the previously published orbit for Janus. These satellites may be major members of an extended ring. 相似文献
3.
Baker AL Baker LR Beshore E Blenman C Castillo ND Chen YP Doose LR Elston JP Fountain JW Gehrels T Kendall JH Kenknight CE Norden RA Swindell W Tomasko MG Coffeen DL 《Science (New York, N.Y.)》1975,188(4187):468-472
For 2 weeks continuous imaging, photometry, and polarimetry observations were made of Jupiter and the Galilean satellites in red and blue light from Pioneer 11. Measurements of Jupiter's north and south polar regions were possible because the spacecraft trajectory was highly inclined to the planet's equatorial plane. One of the highest resolution images obtained is presented here along with a comparison of a sample of our photometric and polarimetric data with a simple model. The data seem consistent with increased molecular scattering at high latitudes. 相似文献
4.
Physical mapping of a translocation breakpoint in neurofibromatosis 总被引:13,自引:0,他引:13
J W Fountain M R Wallace M A Bruce B R Seizinger A G Menon J F Gusella V V Michels M A Schmidt G W Dewald F S Collins 《Science (New York, N.Y.)》1989,244(4908):1085-1087
The gene for von Recklinghausen neurofibromatosis (NF1), one of the most common autosomal-dominant disorders of humans, was recently mapped to chromosome 17 by linkage analysis. The identification of two NF1 patients with balanced translocations that involved chromosome 17q11.2 suggests that the disease can arise by gross rearrangement of the NF1 locus, and that the NF1 gene might be identified by cloning the region around these translocation breakpoints. To further define the region of these translocations, a series of chromosome 17 Not I-linking clones has been mapped to proximal 17q and studied by pulsed-field gel electrophoresis. One clone, 17L1 (D17S133), clearly identifies the breakpoint in an NF1 patient with a t(1;17) translocation. A 2.3-megabase pulsed-field map of this region was constructed and indicates that the NF1 breakpoint is only 10 to 240 kilobases away from 17L1. This finding prepares the way for the cloning of NF1. 相似文献
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DT Rodbell GO Seltzer DM Anderson MB Abbott DB Enfield JH Newman 《Science (New York, N.Y.)》1999,283(5401):516-520
Debris flows have deposited inorganic laminae in an alpine lake that is 75 kilometers east of the Pacific Ocean, in Ecuador. These storm-induced events were dated by radiocarbon, and the age of laminae that are less than 200 years old matches the historic record of El Nino events. From about 15,000 to about 7000 calendar years before the present, the periodicity of clastic deposition is greater than or equal to 15 years; thereafter, there is a progressive increase in frequency to periodicities of 2 to 8.5 years. This is the modern El Nino periodicity, which was established about 5000 calendar years before the present. This may reflect the onset of a steeper zonal sea surface temperature gradient, which was driven by enhanced trade winds. 相似文献
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Tennessen JA Bigham AW O'Connor TD Fu W Kenny EE Gravel S McGee S Do R Liu X Jun G Kang HM Jordan D Leal SM Gabriel S Rieder MJ Abecasis G Altshuler D Nickerson DA Boerwinkle E Sunyaev S Bustamante CD Bamshad MJ Akey JM;Broad GO;Seattle GO;NHLBI Exome Sequencing Project 《Science (New York, N.Y.)》2012,337(6090):64-69
As a first step toward understanding how rare variants contribute to risk for complex diseases, we sequenced 15,585 human protein-coding genes to an average median depth of 111× in 2440 individuals of European (n = 1351) and African (n = 1088) ancestry. We identified over 500,000 single-nucleotide variants (SNVs), the majority of which were rare (86% with a minor allele frequency less than 0.5%), previously unknown (82%), and population-specific (82%). On average, 2.3% of the 13,595 SNVs each person carried were predicted to affect protein function of ~313 genes per genome, and ~95.7% of SNVs predicted to be functionally important were rare. This excess of rare functional variants is due to the combined effects of explosive, recent accelerated population growth and weak purifying selection. Furthermore, we show that large sample sizes will be required to associate rare variants with complex traits. 相似文献
10.
AIM: Our purpose was to induce MSCs differentiating into endothelial cells (EC) in vitro and to provide the seed cells for study of cardiovascular tissue-engineering. METHODS: MSCs were separated by gradient centrifugation on Percoll (density 1 073 g/L) from human bone marrow (HBM), and incubated for purification and amplification in DMEM (low glucose) with 10% fetal bovine serum (FBS). Then, the MSCs were incubated for orientation differentiated into EC in DMEM (high glucose) with 20% FBS, VEGF (10 μg/L), bFGF (5 μg/L), L-glutamine (2 mmol/L), penicillin (1×105U/L) and streptomycin (100 mg/L) for about 14-21 days and their phenotypic characteristics were analyzed by flow cytometry. Afterwards, the differentiating cells were evaluated by histology and immunohistochemistry. RESULTS: The quantity of MSCs was increased from 5.0×105 in the primary culture to 8.0×1012, or to increase to 1.6×107 times after 15 generations of incubation. The purity of MSCs was above 95% and 98% homogeneous at passages 2 and 3, respectively. About 80%-90% of the differentiating cells from MSCs after 14-21 days were positively stained for Ⅷ factor (vWF) related antigen by immunohistochemistry assay, and Weible-palade corpuscle was also observed by transmission electron microscopy in the cytoplasm. CONCLUSION: MSCs from HBM have the capability of differentiation into ECs in vitro, which may be a potential source of seed cells for fabrication of tissue-engineering heart valve, particularly in children with congenital heart disease. 相似文献