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CH Annandale PC Irons VP Bagla UI Osuagwuh EH Venter 《Reproduction in domestic animals》2010,45(2):250-255
The objectives of this work were to determine the site of persistence of lumpy skin disease virus (LSDV) in bulls shedding the virus in semen for a period longer than 28 days, to determine if the virus is present in all fractions of semen and to study lesions that developed in the genital tract. Six serologically negative postpubertal bulls were experimentally infected with a virulent field isolate of LSDV. The polymerase chain reaction (PCR) was performed on sheath washes, vesicular fluid, supernatant and cell‐rich fractions of semen from day 10 to day 26 postinfection (p.i.). Bulls that were positive by PCR on the whole semen sample collected on day 28 p.i. were slaughtered and tissue samples from their genital tracts submitted for histopathological evaluation, immunoperoxidase staining, virus isolation and PCR. Two of the bulls developed severe lumpy skin disease (LSD) and were found to be shedding viral DNA in their semen on day 28 p.i. Viral DNA was identified in all semen fractions from all bulls, but mostly from the cell‐rich fraction and from the severely affected bulls. The PCR assay was positive on postmortem samples of testes and epididymides from the two severely affected bulls. Virus could be recovered from the testes of these two bulls and from the epididymis of one of them. Immunoperoxidase staining was positive for LSDV staining in sections of testes and epididymides exhibiting necrosis. This study suggests that the testis and epididymis are sites of persistence of LSDV in bulls shedding virus in semen for prolonged periods and revealed that viral DNA is present in all fractions of the ejaculate. 相似文献
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S Gür G Türk E Demirci A Yüce M Sönmez Ş Özer EH Aksu 《Reproduction in domestic animals》2011,46(2):289-295
The aim of this study was to determine the changes in diameter of corpus luteum (CL), maternal progesterone (P) concentration, lipid peroxidation and non‐enzymatic antioxidant levels along with enzymatic antioxidant activities in pregnant ewes bearing single and twin foetuses. The ewes were selected from healthy animals that were brought to the abattoir for slaughtering. The ewes were divided into three groups: Group 1 (non‐pregnant, non‐oestrous, n = 30), Group 2 (pregnant bearing a single foetus, n = 30) and Group 3 (pregnant bearing twin foetuses, n = 12) after they were slaughtered. Pregnant ewes were in the first half of the pregnancy. The diameter of CL and P concentration of pregnant ewes bearing a single foetus or twin foetuses were found higher than that found in non‐pregnant ewes. Similarly, the P concentration of pregnant ewes bearing twin foetuses was higher than that found in pregnant ewes bearing a single foetus. Malondialdehyde (MDA) level in pregnant ewes bearing twin foetuses was higher than that found in both non‐pregnant and pregnant ewes bearing a single foetus. The serum glutathione (GSH) level and glutathione‐peroxidase (GSH‐Px) activity of pregnant ewes bearing twin foetuses were found lower than that found in non‐pregnant ewes. Additionally, the GSH‐Px activity of pregnant ewes bearing twin foetuses was found lower than that found in pregnant ewes bearing a single foetus. No significant difference was found between pregnant ewes bearing female and male foetus with respect to diameter of CL, P concentration and oxidative stress parameters. There were significant positive correlations between foetal number (0, 1, 2) and diameter of CL, P concentration, MDA level, and between P concentration and diameter of CL, MDA level. However, significant negative correlations were found between foetal number (0, 1, 2) and GSH level, GSH‐Px activity, and between P concentration and GSH‐Px activity. In conclusion, the diameter of CL enlarges, P production increases and oxidant/antioxidant balance impairs because of the gestation stress in ewes during pregnancy. 相似文献
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Lina Bernaola Molly Darlington Kadie Britt Patricia Prade Morgan Roth Adrian Pekarcik Michelle Boone Dylan Ricke Anh Tran Joanie King Kelly Carruthers Morgan Thompson John J Ternest Sarah E Anderson Scott W Gula Kayleigh C Hauri Jacob R Pecenka Sajjan Grover Heena Puri Surabhi Gupta Vakil 《Journal of insect science (Online)》2021,21(2)
The 2020 Student Debates of the Entomological Society of America (ESA) were live-streamed during the Virtual Annual Meeting to debate current, prominent entomological issues of interest to members. The Student Debates Subcommittee of the National ESA Student Affairs Committee coordinated the student efforts throughout the year and hosted the live event. This year, four unbiased introductory speakers provided background for each debate topic while four multi-university teams were each assigned a debate topic under the theme ‘Technological Advances to Address Current Issues in Entomology’. The two debate topics selected were as follows: 1) What is the best taxonomic approach to identify and classify insects? and 2) What is the best current technology to address the locust swarms worldwide? Unbiased introduction speakers and debate teams began preparing approximately six months before the live event. During the live event, teams shared their critical thinking and practiced communication skills by defending their positions on either taxonomical identification and classification of insects or managing the damaging outbreaks of locusts in crops. 相似文献
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Jocelyn R Holt Lina Bernaola Kadie E Britt Chirs McCullough Morgan Roth Jennie Wagner Max Ragozzino Leslie Aviles Zhilin Li Forest Huval Manoj Pandey Benjamin W Lee Megan Asche Abigail Hayes Abigail Cohen Adrian Marshall Hannah E Quellhorst Rachel V Wilkins Valerie Nguyen Jacqueline Maille Rachel K Skinner John J Ternest Sarah Anderson Scott W Gula Kayleigh Hauri Julius Eason Megan Mulcahy Scott Lee James Michael Villegas Patricia Shorter 《Journal of insect science (Online)》2020,20(5)
Every year, the Student Debates Subcommittee (SDS) of the Student Affairs Committee (SAC) for the annual Entomological Society of America (ESA) meeting organizes the Student Debates. This year, the SAC selected topics based on their synergistic effect or ability to ignite exponential positive change when addressed as a whole. For the 2019 Student Debates, the SAC SDS identified these topic areas for teams to debate and unbiased introduction speakers to address: 1) how to better communicate science to engage the public, particularly in the area of integrated pest management (IPM), 2) the influential impacts of climate change on agriculturally and medically relevant insect pests, and 3) sustainable agriculture techniques that promote the use of IPM to promote food security. Three unbiased introduction speakers gave a foundation for our audience to understand each debate topic, while each of six debate teams provided a strong case to support their stance or perspective on a topic. Debate teams submitted for a competitive spot for the annual ESA Student Debates and trained for the better part of a year to showcase their talents in presenting logical arguments for a particular topic. Both the debate teams and unbiased introduction speakers provided their insight toward a better understanding of the complexities of each topic and established a foundation to delve further into the topics of science advocacy and communication, climate change, and the many facets of integrated pest management. 相似文献
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Measurements of the partitioning of rhenium (Re) between garnet and silicate liquid from 1.5 to 2.0 gigapascals and 1250 degrees to 1350 degreesC show that Re is compatible in garnet. Oceanic island basalts (OIBs) have lower Re contents than mid-ocean ridge basalt, because garnet-bearing residues of deeper OIB melting will retain Re. Deep-mantle garnetite or eclogite may harbor the missing Re identified in crust-mantle mass balance calculations. Oceanic crust recycled into the upper mantle at subduction zones will retain high Re/Os (osmium) ratios and become enriched in radiogenic 187Os. Recycled eclogite in a mantle source should be easily traced using Re abundances and Os isotopes. 相似文献
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Gruber N Hauri C Lachkar Z Loher D Frölicher TL Plattner GK 《Science (New York, N.Y.)》2012,337(6091):220-223
Nearshore waters of the California Current System (California CS) already have a low carbonate saturation state, making them particularly susceptible to ocean acidification. We used eddy-resolving model simulations to study the potential development of ocean acidification in this system up to the year 2050 under the Special Report on Emissions Scenarios A2 and B1 scenarios. In both scenarios, the saturation state of aragonite ?(arag) is projected to drop rapidly, with much of the nearshore region developing summer-long undersaturation in the top 60 meters within the next 30 years. By 2050, waters with ?(arag) above 1.5 will have largely disappeared, and more than half of the waters will be undersaturated year-round. Habitats along the sea floor will become exposed to year-round undersaturation within the next 20 to 30 years. These projected events have potentially major implications for the rich and diverse ecosystem that characterizes the California CS. 相似文献
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EH Hofmeister CB Mosunic BT Torres AG Ralph PA Moore MR Read 《Veterinary anaesthesia and analgesia》2005,32(4):11-12
Ketamine has been implicated as causing increases in intraocular pressure. The purpose of this study is to document the effects of ketamine, diazepam, and their combination on intraocular pressure (IOP) in normal, unpremedicated dogs. Random-source dogs were assigned to one of five groups of 10 dogs each: ketamine 5 mg kg–1 (KET5), ketamine 10 mg kg–1 (KET10), diazepam 0.5 mg kg–1 (VAL), ketamine 10 mg kg–1 with diazepam 0.5 mg kg–1 (KETVAL), saline 0.1 mL kg–1 (SAL), all given intravenously. A baseline IOP was measured before injection, immediately after injection, and at 5, 10, 15, and 20 minutes following injection. IOP was increased over baseline immediately after injection in the KET5, KET10, and KETVAL groups; at 5, 10, and 15 minutes in the KET5 group; and at 20 minutes in the KETVAL group. The mean IOP change compared to SAL increased immediately after injection and at 5 minutes in the KET5, KET10, and KETVAL groups; at 10 and 15 minutes in the KET5 group, and at 20 minutes in the KETVAL group. The mean IOP increased up to 5.7, 3.2, and 3.1 mm Hg over mean baseline in the KET5, KET10, and KETVAL groups, respectively. All dogs in the KET5 group and the majority in the KETVAL and KET10 groups had an increase in their IOP over baseline. Ketamine caused a clinically and statistically significant elevation in IOP over baseline and compared to SAL. The concurrent addition of diazepam did not blunt this increase. Ketamine should be avoided in dogs with corneal trauma, glaucoma, or in those undergoing intraocular surgery. 相似文献
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Mantle plumes and entrainment: isotopic evidence 总被引：8，自引：0，他引：8
Many oceanic island basalts show sublinear subparallel arrays in Sr-Nd-Pb isotopic space. The depleted upper mantle is rarely a mixing end-member of these arrays, as would be expected if mantle plumes originated at a 670-kilometer boundary layer and entrained upper mantle during ascent. Instead, the arrays are fan-shaped and appear to converge on a volume in isotopic space characterized by low (87)Sr/(86)Sr and high (143)Nd/(144)Nd, (206)Pb/(204)Pb, and (3)He/(4)He ratios. This new isotopic component may be the lower mantle, entrained into plumes originating from the core-mantle boundary layer. 相似文献