Molecular detection of bat coronaviruses in three bat species in Indonesia     

Ni Luh Putu Indi Dharmayanti  Diana Nurjanah  Harimurti Nuradji  Ibnu Maryanto  Indra Exploitasia  Risa Indriani 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(6)

Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.  相似文献   

Improving siRNA design targeting nucleoprotein gene as antiviral against the Indonesian H5N1 virus     

Risza Hartawan  Dwi Ari Pujianto  Ni Luh Putu Indi Dharmayanti  Amin Soebandrio 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(2)

BackgroundSmall interfering RNA technology has been considered a prospective alternative antiviral treatment using gene silencing against influenza viruses with high mutations rates. On the other hand, there are no reports on its effectiveness against the highly pathogenic avian influenza H5N1 virus isolated from Indonesia.ObjectivesThe main objective of this study was to improve the siRNA design based on the nucleoprotein gene (siRNA-NP) for the Indonesian H5N1 virus.MethodsThe effectiveness of these siRNA-NPs (NP672, NP1433, and NP1469) was analyzed in vitro in Marbin-Darby canine kidney cells.ResultsThe siRNA-NP672 caused the largest decrease in viral production and gene expression at 24, 48, and 72 h post-infection compared to the other siRNA-NPs. Moreover, three serial passages of the H5N1 virus in the presence of siRNA-NP672 did not induce any mutations within the nucleoprotein gene.ConclusionsThese findings suggest that siRNA-NP672 can provide better protection against the Indonesian strain of the H5N1 virus.  相似文献   

Antimicrobial resistance in Escherichia coli isolated from brown rats and house shrews in markets,Bogor, Indonesia     

Hoang Le HUY  Nobuo KOIZUMI  Harimurti NURADJI  Susanti  Susan M. NOOR  NLP Indi DHARMAYANTI  Takeshi HAGA  Kazuhiro HIRAYAMA  Kozue MIURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):531

The prevalence of antimicrobial resistance (AMR) in small mammals dwelling in the city was used as an indicator of AMR bacteria in the environment. We captured 87 small mammals (79 brown rats and 8 house shrews) in four markets, Bogor, Indonesia in October 2019, and we obtained 20 AMR Escherichia coli (E. coil) from 18 brown rats and two house shrews. Of these, eight isolates were determined to be multi-drug resistant (MDR) E. coli, suggesting the potential contamination of AMR E. coli in the markets in Bogor, Indonesia, and that mammals, including humans, are at risk of infection with AMR E. coli from environment.  相似文献