Antimicrobial and cytotoxic activity of Ruta graveolens     

Ivanova A  Mikhova B  Najdenski H  Tsvetkova I  Kostova I 《Fitoterapia》2005,76(3-4):344-347

The methanol, petroleum ether, ethyl acetate and water-methanol extracts of Ruta graveolens were found to possess antimicrobial and cytotoxic activities.  相似文献   

New procedure for the simulation of belowground competition can improve the performance of forest simulation models     

Vladimir Shanin  Raisa Mäkipää  Maxim Shashkov  Natalya Ivanova  Konstantin Shestibratov  Svetlana Moskalenko  Liliya Rocheva  Pavel Grabarnik  Kapitolina Bobkova  Alexey Manov  Andrey Osipov  Elvira Burnasheva  Maria Bezrukova 《European Journal of Forest Research》2015,134(6):1055-1074

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Spontaneous tomato fruit size,shape, and weight mutations and traits evolution     

A. Yu. Avdeyev  O. P. Kigashpaeva  Yu. I. Avdeyev  L. M. Ivanova 《Russian Agricultural Sciences》2014,40(2):96-99

The results of study of spontaneous fruit size and shape mutants and inheritance of these traits in tomato are given and genetic processes are explained. It is concluded that the diversity of tomato fruit shape and weight in the collection is due mainly to mutations of genes—diameter of fruit width and diameter of height joined into an ovate cluster.  相似文献   

Doubled haploid production of sunflower (Helianthus annuus L.) through irradiated pollen-induced parthenogenesis     

M. Todorova  P. Ivanov  P. Shindrova  M. Christov  I. Ivanova 《Euphytica》1997,97(3):249-254

Induced parthenogenesis as a possible method of haploidization in sunflower (H. annuus L.) was tested in previous investigations (Todorova et al., 1994) and conditions for reproducible regeneration of gynogenic doubled haploids were established by the present work. Forty eight treatments were studied, involving four pollen donors and four recipient hybrids. Pollen was irradiated with doses of 300 Gy, 600 Gy and 900 Gy. In total, 2279 embryos were cultivated in vitro of which 1107 plants were obtained and 582 of them produced seeds after selfing. The ploidy level of the regenerants was evaluated at the two – three leaf stage and 296 of the plantlets obtained were haploids. Some of them underwent spontaneous diploidization, the others were treated with colchicine solution for chromosome doubling. The diploid plantlets were checked for their gynogenic origin by genetical and biochemical methods. The effectiveness of the method expressed as the number of agronomically useful DH lines to the number obtained that were fertile and resistant to downy mildew, branched and unbranched plants was 8,6% on average. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

New furostanol saponins from Smilax aspera L. and their in vitro cytotoxicity     

Ivanova A  Mikhova B  Batsalova T  Dzhambazov B  Kostova I 《Fitoterapia》2011,82(2):282-287

The occurrence of the two new cis-fused A/B rings furostanol saponins (25S)-26-O-β-d-glucopyranosyl-5β-furostan-1β,3β,22α,26-tetraol-1-O-β-d-glucopyranoside and (25S)-26-O-β-d-glucopyranosyl-5β-furostan-1β,2β,3β,5β,22α,26-hexaol and the known compounds (25S)-26-O-β-d-glucopyranosyl-5β-furostan-3β,22α,26-triol-3-O-α-l-rhamnopyranosyl-(1→2)-O-β-d-glucopyranosyl-(1→2)-O-β-d-glucopyranoside and (25S)-26-O-β-d-glucopyranosyl-5β-furostan-3β,22α,26-triol-3-O-β-d-glucopyranosyl-(1→2)-O-β-d-glucopyranoside, trans-resveratrol, (+) catechin and (−) epicatechin in the rhizomes of Smilax aspera is reported. All saponins have been isolated as their 22-OMe derivatives, which were further subjected to extensive spectroscopic analysis. The isolated furostanol saponins were evaluated for cytotoxic activity against human normal amniotic and human lung carcinoma cell lines using neutral red and MTT assays. In vitro experiments showed significant cytotoxicity in a dose dependent manner with IC₅₀ values in the range of 32.98-94.53 µM.  相似文献   

Genetic polymorphism of the bovine leukemia virus in the Russian federation     

M. I. Gulyukin  N. G. Kozyreva  L. A. Ivanova  T. V. Stepanova  I. A. Gulyukina 《Russian Agricultural Sciences》2016,42(6):472-475

Nucleotide sequence of a section of the Env 105 gene of bovine leukemia provirus isolates, obtained from farm animals in different regions of Russia, was determined. Conducted phylogenetic analysis has allowed us to assess heterogeneity of the studied population of the bovine leukemia virus (BLV). Based on results of BLV genetic diversity, four virus’s genotypes—I, II, IV, and VII with dominating genotype IV—were detected.  相似文献   

Rapid vegetative propagation of conifers     

Z. Ivanova 《Scientia Horticulturae》1981,14(4):347-355

Factors influencing the rooting-potential of stem cuttings of conifers were studied for the purpose of improving methods of rapid vegetative propagation. Within the bounds of hereditary limits, root regeneration depends, to a considerable extent, on the qualities of rooting-media, date of cutting excision, age and physiological conditions of the stock plant, age of the cutting-wood, its position on the plant, and chemical treatment. Hardwood cuttings, which have a relatively high content of endogenous auxin and carbohydrates, and morphological root initials, gave the best results. This method made it possible to increase the growth rate of cuttings 2.5–5 times that of cuttings taken from shoots of current growth.  相似文献   

On further measures of brucellosis control     

V I Ivanova 《Veterinariia》1965,42(11):30-32

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Mechanism of the relation between the content of nitro and nitroso compounds in amnion and allantois of chicken embryos and rate of posthatch chick growth     

V. Yu. Titov  N. S. Akimova  V. I. Fisinin  A. V. Ivanova 《Russian Agricultural Sciences》2012,38(1):47-50

It is established that metabolism of nitric oxide (NO) in the chicken embryo and posthatch growth rate of chicks have a direct relation which is determined both by the genotype and by external factors influencing posthatch growth rate.  相似文献   

Culture results from milk samples submitted to veterinary diagnostic laboratories from August 2003 to December 2006 in New Zealand     

KR Petrovski  NB Williamson  N Lopez-Villalobos  TJ Parkinson  IG Tucker 《New Zealand veterinary journal》2013,61(6):317-322

Abstract

AIMS: To determine the pattern of isolation of major mastitis-causing organisms isolated from milk samples submitted to five veterinary diagnostic laboratories in New Zealand.

METHODS: The culture results of 25,288 milk samples that were collected from dairy cows throughout New Zealand from August 2003 to December 2006 and submitted to a group of veterinary diagnostic laboratories were assembled, reviewed and summarised. Logistic regression was used to analyse the effect of year, region (i.e. North vs South Island), and season on the probability of isolating the two most common organisms.

RESULTS: The most commonly isolated mastitis causing organisms from all samples were: Streptococcus uberis (23.6%), Staphylococcus aureus (23.5%), coagulase-negative staphylococci (CNS; 7.2%), Strep. dysgalactiae (6.2%), Bacillus spp. (4.0%), and coliforms (3.7%). The percentage of samples with isolates of Strep. uberis or Staph. aureus was affected by island, year and season (p<0.001). For most of the year, except in late winter and early spring when Strep. uberis was much more common, the percentage of isolates of Strep. uberis and Staph. aureus were not apparently different despite the former being an environmental pathogen and the other a contagious one.

CONCLUSION: The pattern of isolation of major mastitis-causing organisms, as determined from culture of milk samples submitted to diagnostic laboratories in New Zealand, has changed significantly over the last 40 years, with a substantial increase in the percentage of isolates that are Strep. uberis and a decrease in isolates of Strep. agalactiae. There is a clear seasonal pattern to the isolation of both Strep. uberis and Staph. aureus, particularly the former.

CLINICAL RELEVANCE: Knowledge of the aetiological agents causing bovine mastitis on a farm is of value in determining the choice of treatment. This dataset shows that, although there is seasonal pattern to the isolation of mastitis-causing organisms in New Zealand, both Strep. uberis and Staph. aureus are isolated throughout the year, so bacteriology is of value in determining aetiology even in late winter/early spring.  相似文献