用简易诊断法检查判断液压系统各元件的工作性能     

李理  阚志新  李阁萍 《农机化研究》2006,(5):184-185,188

故障的排除首先在于判断，目前基层维修网点大多数没有检测仪器和设备，出了故障乱拆乱卸，导致液压元件工作失灵，甚至整车液压系统瘫痪。拖拉机液压系统产生故障的主要原因是液压元件工作性能恶化引起的。为此，介绍了在没有检测仪器和设备的条件下，如何应用简易诊断法检查判断各液压元件的工作性能。  相似文献   

粉红粘帚霉67-1菌株寄生核盘菌研究   总被引：5，自引：0，他引：5  

张拥华  高会兰  马桂珍  李世东 《植物病理学报》2004,34(3):211-214

 用诱捕法从海南乐东县菜园土壤中获得一株对核盘菌菌核具有强寄生能力的粉红粘帚霉(Gliocladium roseum)菌株67-1,寄生频率为100%。该菌的PDA平板回接核盘菌菌核,一周后寄生率可达100%。对峙培养发现其对核盘菌有明显的抑菌带。保湿条件下,该菌孢子在24h内成功侵入核盘菌菌核。切片显微观察证明该菌能高效侵染菌核,造成组织溃解。寄生过程中菌核内蛋白组成发生明显变化。这一菌株在22~35℃均能很好生长,菌丝及产孢最适温度为24℃,产孢量大。认为这一菌株具有良好的菌核病生防应用潜力。  相似文献   

Preparation and Identification of Canine Parvovirus NY Strain VP2 Protein Polyclonal Antibody     

MAO Qian-qian  ZHOU Ling  TANG Qing-hai  BU Bin  TANG Cun-duo  JIAO Zhu-jin  YAO Lun-guang  KAN Yun-chao  YANG Jian-wei  CUI Shang-jin 《中国畜牧兽医》2016,43(7):1659-1666

This study was aimed to prepare canine parvovirus (CPV) VP2 protein polyclonal antibody.The recombinant expression vector pET28a-CPV-VP2 was constructed and transfromed into E.coli BL21 (DE3),the expression of recombinant proteins was induced by IPTG from which the fusion protein was identified by SDS-PAGE.The target protein was purified and emulsify with adjuvant,the prepared immunogen was inoculated into rabbit by subcutaneous injections to prepare of VP2 protein specific polyclonal antibody.The immuno-activity,titers,neutralization titers of the prepared polyclonal antibody were determined by immunoperoxidase monolayer assay (IPMA).The results showed that the expressed recombinant protein VP2 (rVP2) existed in the form of inclusion body with a molecular weight of 72 ku.The prepared polyclonal antibody titer was 1 600 dilution,the virus titer was 10⁷ TCID₅₀/mL,the neutralizing titer was 1∶2 884.The antibodies showed specific reaction with CPV.In conclusion,rVP2 specific polyclonal antibody showed wonderful immunocompetence,specificity and neutralizing activity,providing foundation for the development of genetic vaccine and clinical therapeutic method.  相似文献   

介电式脱绒棉种分选机的研制   总被引：7，自引：0，他引：7  

张晓海  坎杂  王丽红  田学艳  曲金丽 《中国农机化》2004,(5):33-35

为了提高棉花种子的发芽率，采用了先进的计算机控制技术，研制了介电式棉种分选机，达到了利用种子的介电特性进行分选的目的。试验表明，该机工作稳定可靠，操作方便．完全满足种子精选的要求，具有广泛的推广前景。  相似文献   

奶牛饲喂技术与设备的现状分析   总被引：1，自引：0，他引：1  

谭春林  坎杂  曾明军  李景彬 《农机化研究》2007,(12):240-242,245

随着我国奶牛养殖业的高速发展,对奶牛饲喂机械化设备的需求较为迫切.为此,介绍了传统饲喂、TMR饲喂和饲喂机器人等3种饲喂技术,并对其配套设备的优缺点进行了分析,指出了以个体奶牛体况的精细化饲养来实现数字化养殖是将来的发展趋势.  相似文献   

宁夏引黄灌区小麦垄作节水高产栽培研究   总被引：5，自引：0，他引：5  

袁汉民  王小亮  孙建昌  陈东升  赵桂珍 《节水灌溉》2005,(6):5-7

在宁夏引黄灌区进行小麦垄作栽培试验：春小麦全生育期在降水量为89.2 mm条件下,灌水量为3 183m3/hm^2,比传统的平作栽培的小麦灌水量5 131.5 m3/hm^2节水38%,抗倒伏,锈病、白粉病等发病轻;冬小麦全生育期在降雨量为101 mm、灌水量为4 582.5m3/hm^2条件下,4个冬小麦品种（系）垄作栽培试验的单产为6 691.5～7 350kg/hm2,垄作小麦播种量为210kg/hm2的处理比播种量为240kg/hm^2处理省种30%,而产量提高0.5%～7.9%.“烟农19号”和2个新培育的冬小麦品系2002AW5012、2002AW5011适于垄作,其产量水平为9 295.5～9 636kg/hm^2,比对照品种“宁冬6号”增产10.5%～14.5%.  相似文献   

大型悬吊井筒抽芯式泵机组安装质量控制     

阚永庚 《排灌机械》2006,24(5):20-24,28

由于悬吊井筒抽芯式泵机组结构不同于传统的苏式泵，按原有的规范和要求不能保证该型机组安装检修质量．仅以厂家提供的说明书要求进行安装，因制造工艺、精度等原因，运行过程中，机组稳定性差，故障率高等一直困扰着使用单位．为保证机组安装质量，消除机组运行中常见的不正常现象和故障，以64LKXA-24．5型立式泵机组为例，针对该型机组结构形式的特殊性，根据规范的安装要求，结合安装检修过程中存在的问题，介绍了该形式泵机组的主要安装方法，提出了相应的注意事项．  相似文献   

Comparison and analysis of the staining methods for cholesterol     

YUAN An  NIE Kun  KAN Bo-hong  HAN Jing-xian  YU Jian-chun 《园艺学报》2010,26(8):1654-1657

Cholesterol staining is a useful approach for the visualization, localization and quantification of cholesterol in cells or tissues, which is frequently used to investigate the mechanisms of some diseases such as arteriosclerosis, Niemann-Pick disease type C, and Alzheimer's disease. It can be accomplished through various microscopes including light microscope, fluorescent microscope, and electronic microscope. During the past decades, various types of methods for cholesterol staining with different principles have been established for different applications. It is important to choose an appropriate method that is suitable for particular experimental aims, features and conditions. At present, three kinds of methods are frequently applied: filipin fluorescent method, BCθ(a biotinylated and carlsberg protease-nicked derivative of perfringolysin O) toxin method, and cholesterol oxidase-diaminobenzidine(oxidase-DAB) method. Four kinds of methods are scarcely applied: Schultze method, perchloric acid-naphthoquinone method(PAN), digitonin method, and o-phthalaldehyde method. In this review, the principles, advantages, and disadvantages of these methods are compared with the emphasis of the application, sensitivity, and specificity.  相似文献   

香石竹斑驳病毒单克隆抗体的制备及检测应用     

吴建祥  王贵珍  洪健  张仲凯  周雪平 《植物病理学报》2005,35(4):322-326

 用香石竹斑驳病毒(CarMV)免疫的BALB/c鼠脾细胞与Sp2/0鼠骨髓瘤细胞融合,经筛选克隆,获得5株能稳定传代且分泌抗CarMV单克隆抗体(MAb)的杂交瘤细胞,并分别制备它们的单克隆抗体腹水。5株单克隆抗体腹水间接ELISA效价达10^-6,其中3G1、1B9、2A9和2F8的抗体类型及亚类均为IgG1,而2F₂为IgG3。Western-blot分析表明,5株单克隆抗体均与CarMV 38 kD的外壳蛋白亚基有特异性反应。利用2A9单抗建立的抗原包被的间接ELISA (ACP-ELISA)检测CarMV方法,病叶1:800倍稀释、提纯CarMV病毒浓度为1 ng/mL (绝对检测量为0.1 ng)时仍能检测到病毒。利用ACP-ELISA对田间香石竹样品的检测表明,CarMV在香石竹上发病很普遍。  相似文献   

丝绸之路南道旅游资源开发研究-以和田地区为例     

雷军  阚耀平  许英勤  赵兴有 《干旱区资源与环境》2005,19(5):140-145

丝绸之路南道不论在自然旅游资源,还是在人文旅游资源方面都有其独特之处。本文通过对和田地区旅游资源和客源市场现状分析,提出围绕丝绸之路文化旅游为核心,突出以玉石故乡,大漠风光,田园绿洲,佛国古城及伊期兰教四大特色为主题,重点推出“和田玉石文化”、“尼雅寻梦”等精品,以此为龙头,带动区域旅游业发展的旅游资源开发策略。并提出“一主线,六干线”的各具特色的旅游线路。  相似文献