First Established Pregnancies in Mediterranean Italian Buffaloes (Bubalus bubalis) Following Deposition of Sexed Spermatozoa near the Utero Tubal Junction     

GA Presicce  S Verberckmoes  EM Senatore  P Klinc  D Rath 《Reproduction in domestic animals》2005,40(1):73-75

At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose.  相似文献   

ARSENIC POISONING IN CATTLE     

M. W. McLENNAN B.V.Sc.  M.Sc.  M. E. DODSON B.V.Sc.  J. KELLY B.V.Sc. 《Australian veterinary journal》1972,48(6):367-367

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Pleuritis in the Dog Associated with Actinomyces-like Organisms     

J. D. COLLINS  T. D. GRIMES  W. R. KELLY  J. K. KEALY  E. C. MURPHY 《The Journal of small animal practice》1968,9(10):513-518

Abstract— The clinico-pathological and radiological features of two cases of effusive canine pleuritis are presented. In both instances Actinomyces-like organisms were encountered but attempts to culture them (using aerobic and anaerobic methods) were unsuccessful. In one case Salmonella dublin was obtained in cultures at all stages of the investigations. The zoonotic implications of these and simiIar cases are discussed.
Résumé— GL'auteur expose les caractéristiques clinico-pathologiques et radiologiques de la pleurite exsudative, observée chez deux chiens. Dans l'un et l'autre cas ont été décelés des organismes semblables à l'actinomycès, mais les tentatives de culture (en milieu aerobie et anaerobie) ont échoué. Dans l'un des cas, Salmonella dublin a été obtenue à tous les stades de l'investigation. L'auteur examine les aspects zoonotiques des deux cas étudiés et de cas semblables.
Zusammenfassung— Die klinisch-pathologischen und die radiologischen Befunde von zwei Fällen von Pleuritis exsudativa des Hundes werden dargestellt. In beiden Fällen wurden astinomy-cesähnliche Organismen gefunden, aber die Kulturversuche (mit aeroben und anaeroben Methoden) verliefen erfolglos. In einem Fall wurde Salmonella dublin in Kulturen aller Stadien der Untersuchung erhalten. Die zoonotische Bedeutung dieser und ähnlicher Fälle wird besprochen.  相似文献   

Corrigendum to "A DNA vaccine coding for glycoprotein B of pseudorabies virus induces cell-mediated immunity in pigs and reduces virus excretion early after infection"     

van Rooij EM  Haagmans BL  Glansbeek HL  de Visser YE  de Bruin MG  Boersma W  Bianchi AT 《Veterinary immunology and immunopathology》2000,75(1-2):161-162

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Impact of foot-and-mouth disease on mastitis and culling on a large-scale dairy farm in Kenya     

Nicholas A Lyons  Neal Alexander  Katharina DC St?rk  Thomas D Dulu  Jonathan Rushton  Paul EM Fine 《Veterinary research》2015,46(1)

Foot and mouth disease (FMD) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into FMD-free countries. Information on the impact of the disease in FMDV-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. A FMD (virus serotype SAT2) outbreak on a large-scale dairy farm in Nakuru County, Kenya provided an opportunity to evaluate the impact of FMD on clinical mastitis and culling rate. A cohort approach followed animals over a 12-month period after the commencement of the outbreak. For culling, all animals were included; for mastitis, those over 18 months of age. FMD was recorded in 400/644 cattle over a 29-day period. During the follow-up period 76 animals were culled or died whilst in the over 18 month old cohort 63 developed clinical mastitis. Hazard ratios (HR) were generated using Cox regression accounting for non-proportional hazards by inclusion of time-varying effects. Univariable analysis showed FMD cases were culled sooner but there was no effect on clinical mastitis. After adjusting for possible confounders and inclusion of time-varying effects there was weak evidence to support an effect of FMD on culling (HR = 1.7, 95% confidence intervals [CI] 0.88-3.1, P = 0.12). For mastitis, there was stronger evidence of an increased rate in the first month after the onset of the outbreak (HR = 2.9, 95%CI 0.97-8.9, P = 0.057).

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-015-0173-4) contains supplementary material, which is available to authorized users.  相似文献   

Effect of lytic peptides on selected fish bacterial pathogens*     

D. KELLY  W. R. WOLTERS  J.M. JAYNES 《Journal of fish diseases》1990,13(4):317-321

Abstract. Two synthetic lytic peptides, derivatives of the naturally occurring lytic peptide cecropin B, were tested for their effect on eight pathogenic fish bacteria. All bacteria were grown on tryptic soy agar at 30°C. Four levels (dilutions) of lytic peptides were incubated with viable cells in the log phase for 1 h. Standard plate counts were made after 24±2h with control plates (no toxin) representing 100% survival. Significant differences (P<0.05) in the toxicity of the two peptides were found for six out of eight bacterial pathogens. Peptide concentration or dilution level also caused significant difference (P<0.01) in bacterial counts. Peptide type by dilution level interactions were significant (P<0.01) for only two bacterial pathogens. The LD₅₀. in molar concentrations of the lytic peptides on the bacterial pathogens, ranged from approximately 10^?6 to 10^?9. The possible role of utilizing these peptides to enhance disease resistance in aquaculture is discussed.  相似文献   

Epidermal hyperplasia of walleye, Stizostedion vitreum vitreum (Mitchill), associated with retrovirus-like type-C particles: prevalence, histologic and electron microscopic observations     

T. YAMAMOTO  R. K. KELLY  O. NIELSEN 《Journal of fish diseases》1985,8(5):425-436

Epidermal hyperplasia consisting of discrete translucent raised outgrowths of cells were observed on the skin of walleye, Stizostedion vitreum vitreum (Mitchill), during their spawning period in the spring. The cells constituting the hyperplastic growths were limited to the epidermal layer, and were associated with surface budded, 120-nm-diameter, retrovirus-like particles located in the expanded intercellular spaces. These tumour-like growths were distinct from the other virus-associated skin lesions of walleye including dermal sarcoma, lymphocystis disease and herpesvirus-associated hyperplasia. Lesions could be differentiated by careful observation in the field and comparison of portions of each growth by histologic and electron microscopic observations.  相似文献   

Isometric twitch tension of frog skeletal muscle as a function of temperature     

KELLY E  FRY WJ 《Science (New York, N.Y.)》1958,128(3317):200-202

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STARRY SKY HEPATIC ULTRASONOGRAPHIC PATTERN IN HORSES     

KELLY L. CARLSON  M. KEITH CHAFFIN  WAYNE V. CORAPI  KAREN F. SNOWDEN  DAVID G. SCHMITZ 《Veterinary radiology & ultrasound》2011,52(5):568-572

The starry sky hepatic pattern is an unusual ultrasonographic appearance of equine liver characterized by numerous small, hyperechoic foci, some of which cast an acoustic shadow, distributed randomly throughout the hepatic parenchyma. Our objectives were to describe the signalment, clinical signs, clinicopathological findings, primary disease process, and ultrasonographic findings of horses with this ultrasonographic pattern, as well as determine the associated gross and histologic changes. The starry sky pattern was identified in 18 adult horses of mixed gender and breed. The horses had various clinical signs, with weight loss and anorexia reported most commonly. Liver size and parenchymal echogenicity were normal in most horses. The hyperechoic foci frequently caused acoustic shadowing. Biliary dilation was noted rarely. The ultrasonographic pattern was the result of numerous fibrosing hepatic granulomas in all horses evaluated histologically. γ‐Glutamyltransferase was the most commonly elevated hepatic enzyme, though it was increased in fewer than half the horses. Fifteen horses had an additional disease that was identified as the apparent cause of clinical signs. Three horses had primary hepatic disease while 12 had diseases of other body systems. Therefore, the starry sky ultrasonographic pattern is likely incidental in most horses and not clinically significant. Improved recognition of this pattern and further investigation of affected horses may help refine the etiology and clinical significance of the granulomas.  相似文献   

PROGRESSIVE ATAXIA IN CHAROLAIS CATTLE     

R. C. W. DANIEL  W. R. KELLY † 《Australian veterinary journal》1982,58(1):32-32

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