排序方式： 共有6条查询结果，搜索用时 31 毫秒
1
1.
Genetic Diversity and Allelic Frequency of Selected Thai and Exotic Rice Germplasm Using SSR Markers
Wanwarang PATHAICHINDACHOTE Natjaree PANYAWUT Kannika SIKAEWTUNG Sujin PATARAPUWADOL Amorntip MUANGPROM 《水稻科学》2019,26(6):393-403
A collection of 167 Thai and exotic rice accessions was subjected for evaluation of genetic diversity and assessment of relationship by simple sequence repeat(SSR) markers. Among a total of 49 SSR markers, 13 markers distributing over 12 rice chromosomes showed clear polymorphic band patterns, and they were selected for genetic assessment. A total of 110 alleles were detected with an average of 8.46 alleles per locus. The averages of gene diversity, heterozygosity and polymorphic information content were 0.59, 0.02 and 0.56, respectively. The unweighted-pair group method with arithmetic averages(UPGMA) clustering analysis was performed for genetic distance, and phylogenetic tree was constructed. The result showed that this rice collection was divided into two major groups, classified as japonica and indica subspecies. Within the japonica group, temperate japonica and tropical japonica subgroups can be clearly separated. Three-dimensional principal component analysis projection and model-based population structure analysis showed consistent clustering results with two major groups of UPGMA analysis, supporting the classification of japonica and indica subspecies. The indica allelic frequency was also investigated to provide an indicative guide for breeders to overcome the practical problems on sterility of inter-subspecies hybrid offspring. This rice collection and information obtained in this study will be useful for rice breeding programs. 相似文献
2.
Kanokwan KAEWMUNGKUN Keasinee TONGMARK Sriprapai CHAKHONKAEN Numphet SANGARWUT Thiwawan WASINANON Natjaree PANYAWUT Khanittha DITTHAB Kannika SIKAEWTUNG QI Yong-bin Sukanya DAPHA Atikorn PANYA Natthaporn PHONSATTA Amorntip MUANGPROM 《农业科学学报》2023,22(3):679-690
Rice is the staple food for about half of the world’s population. Preferred by consumers, aromatic rice is a special type of rice with great commercial value. Cooking and eating qualities and aroma are the major grain qualities favored by most consumers. Currently, most of the available aromatic varieties have low yields and some undesirable agronomic traits. Thus, there is an urgent need to develop better aromatic rice varieties. This work aims to identify rice germplasm lines that have good grain quality and to develop new varieties with desirable traits. Thirty-six out of 188 germplasm lines were found to have betaine aldehyde dehydrogenase 2 (badh2) controlling the aroma and were analyzed for their 2-acetyl-1-pyrroline (2AP) contents. Then, 17 of those lines were found to have alleles for low amylose content and low gelatinization temperature, controlled by waxy and starch synthase IIa (SSIIa), respectively, suggesting that they are aromatic rice lines with high cooking and eating qualities. A total of 158 F7 recombinant inbred lines (RILs) generated from five crosses of the selected germplasm lines were planted for phenotypic and yield observations, resulting in 27 F8 RILs selected for yield evaluation and genotyping. Finally, four out of the seven F9 aromatic RILs showed high yield, high 2AP production, and low amylose content, in agreement with their genotypes. The other three F9 RILs were aromatic rice lines with high amylose content and high yield. Because consumer preferences for grain quality vary depending on regions and ethnic groups, the high-yielding aromatic RILs generated from this study can be used to increase the yield of Thai rice and to raise market value and farm profits. 相似文献
3.
Tawin Inpankaew Tawisa Jiyipong Kannika Wongpanit Nongnuch Pinyopanuwat Wissanuwat Chimnoi Chanya Kengradomkij Xuenan Xuan Ikuo Igarashi Lihua Xiao Sathaporn Jittapalapong 《Tropical animal health and production》2014,46(2):487-490
The objectives of this study were to determine the individual and herd-level prevalence and genotype of Cryptosporidium and to identify putative risk factors associated with Cryptosporidium spp. infections in water buffaloes in northeast Thailand. Fecal samples from 600 water buffaloes of 287 farms in six provinces were collected and tested using DMSO-modified acid-fast staining and polymerase chain reaction. The overall prevalence of Cryptosporidium infections in buffaloes was 5.7 and 8.7 % among individual animals and herds, respectively. The provinces with highest infected Cryptosporidium were located in the Sakon Nakhon Basin in the northern part of the region. In addition, higher herd prevalence was observed among farms with more than five buffaloes (30 %) than those with five or less animals (16.2 %). Thirty (88.2 %) of the 34 Cryptosporidium-positive samples were Cryptosporidium parvum and four (11.8 %) were Cryptosporidium ryanae. 相似文献
4.
5.
6.
Nisakorn Songaksorn Wilaiwan Petsophonsakul Kidsadagon Pringproa Kannika Na Lampang Nattawooti Sthitmatee Nuttawan Srifawattana Kakanang Piyarungsri Kriangkrai Thongkorn 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
BackgroundThe feline viral rhinotracheitis, calicivirus, and panleukopenia (FVRCP) vaccine, prepared from viruses grown in the Crandell-Rees feline kidney cell line, can induce antibodies to cross-react with feline kidney tissues.ObjectivesThis study surveyed the prevalence of autoantibodies to feline kidney tissues and their association with the frequency of FVRCP vaccination.MethodsSerum samples and kidneys were collected from 156 live and 26 cadaveric cats. Antibodies that bind to kidney tissues and antibodies to the FVRCP antigen were determined by enzyme-linked immunosorbent assay (ELISA), and kidney-bound antibody patterns were investigated by examining immunofluorescence. Proteins recognized by antibodies were identified by Western blot analysis.ResultsThe prevalences of autoantibodies that bind to kidney tissues in cats were 41% and 13% by ELISA and immunofluorescence, respectively. Kidney-bound antibodies were observed at interstitial cells, apical border, and cytoplasm of proximal and distal tubules; the antibodies were bound to proteins with molecular weights of 40, 47, 38, and 20 kDa. There was no direct link between vaccination and anti-kidney antibodies, but positive antibodies to kidney tissues were significantly associated with the anti-FVRCP antibody. The odds ratio or association in finding the autoantibody in cats with the antibody to FVRCP was 2.8 times higher than that in cats without the antibody to FVRCP.ConclusionsThese preliminary results demonstrate an association between anti-FVRCP and anti-cat kidney tissues. However, an increase in the risk of inducing kidney-bound antibodies by repeat vaccinations could not be shown directly. It will be interesting to expand the sample size and follow-up on whether these autoantibodies can lead to kidney function impairment. 相似文献
1