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Mahipal S. Shekhawat Narpat S. Shekhawat Harish Kheta Ram Mahendra Phulwaria Amit Kumar Gupta 《Journal of Crop Science and Biotechnology》2011,14(2):133-137
An in vitro propagation method for female plants of Momordica dioica (Roxb.) has been established. The nodal segments were harvested and the cut ends of the explants were sealed with wax and
then surface sterilized and cultured. Bud breaking occurred on Murashige and Skoog’s (MS) agar-gelled medium + 2.0 mg L−1 6-Benzylaminopurine (BAP) + 0.1 mg L−1 Indole-3 acetic acid (IAA). The cultures were amplified by passages on MS medium supplemented with 1.0 mg L−1 BAP + 0.1 mg L−1 IAA. Further, shoot amplification (29.2 shoots per vessel) was achieved by subculturing of in vitro regenerated shoot clump on MS medium + 0.5 mg L−1 BAP + 0.1 mg L−1 IAA. The micropropagated shoots were subsequently transferred for root formation on half-strength MS medium + 2.0 mg L−1 Indole-3 butyric acid (IBA) with 89% success rate. The in vitro-regenerated shoots were also rooted ex vitro with 34% success. These plantlets were hardened in the greenhouse and transferred to the field. The established protocol
is suitable for true to type cloning of mature female plant of M. dioica. 相似文献
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Bilas Singh Mahipal Bishnoi Mana Ram Baloch Genda Singh 《Archives of Agronomy and Soil Science》2013,59(8):1031-1049
A combination of silvicultural species [Prosopis cineraria (L.), Ailanthus excelsa Roxb. and Colophospermum mopane (J. Kirk ex Benth.)] were planted with horticultural species [Ziziphus mauritiana (L.), Cordia myxa (Forster), and Emblica officinalis (Gaertn)] and intercropped with wheat (Triticum aestivum). Z. mauritiana +P. cineraria combination produced greater fruit, fodder and fuel wood and was less competitive to wheat crop. Crop yield reduced by 5% to 23% in the agroforestry systems than the yield in sole crop plot. Lowest yield was in C. mopane + C. myxa combination. Fodder yield was 0.53, 0.20 and 0.07 t ha?1 from C. mopane (cursive), P. cineraria and A. excelsa, respectively, whereas utilizable biomass was 2.63 t ha?1 from C. myxa (cursive) + P. cineraria, 2.21 t ha?1 from C. myxa (cursive) + C. mopane and 2.18 t ha?1 from Z. mauritiana + P. cineraria combinations. Soil organic carbon and NH4–N increased (by 7% and 8%, respectively), whereas NO3–N and PO4–P decreased in agroforestry compared to the sole tree plots. Primary root attributes of P. cineraria, A. excelsa and C. mopane were higher in agroforestry and mostly concentrated in the top 0–25 cm of the soil layer. Z. mauritiana + P. cineraria were the best combination with minimum yield reduction and were found to be beneficial in enhancing soil fertility. 相似文献
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Govindraj Ramakantrao Bhaganagare Mahipal Singh Kesawat Basanta Kumar Das Arumuganainar Suresh Babasaheb Shivmurti Surwase Manorama 《Journal of Crop Science and Biotechnology》2013,16(1):17-22
Rhizobia diversity is considered as one of the most useful resources for bioprospecting due to their symbiotic nitrogen-fixing ability with members of Leguminosae. The highly conserved nature of the nitrogenase reductase gene (nifH) makes it an ideal molecular tool to determine the potential for biological nitrogen fixation in any environment. In the present investigation, 250 rhizobial strains were isolated from legumes belonging to different geographical locations of Chhattisgarh, India. Genetic diversity of the nitrogenfixing bacterial community was analyzed using the nifH gene-specific primer. The polymorphism was found among the nitrogen-fixing population of different sources and origin but not in same source of rhizobia. Further, the symbiotic plasmid DNA was characterized on the basis of size and copy number of plasmids. The plasmid number varying from one to three in different rhizobial isolates had a size greater than 23 kb, while in some rhizobial isolates plasmids were absent. In addition, to examine the role of ascorbate in respiratory protection, the clear black spot margin of ascorbate was observed in the endodermis region of the nodule whereas scarcely dispersed in the infected region. Therefore, our findings demonstrated that knowing the rhizobial nifH gene diversity along with copy number of the plasmid is important for strain identification, deciding its fertility, productivity standards, and potential of biological nitrogen fixation across the geographical region. 相似文献
4.
水稻产量及其相关性状的数量性状位点(QTL)鉴定(英文) 总被引：2，自引：0，他引：2
Deshmukh Rupesh Sonah Humira Tiwari Anshuman Mahatman Krityanand Jadhav Srikrishna Kadam Suhas Kesawat Mahipal Reddy Niranjan Deshmukh Nilesh Verulkar Satish 《广西农业科学》2008,39(5)
由于大部分水稻育种的最终目标是获得高产、稳产,因此对于育种工作者来说．水稻产量及其构成因素 是重要的性状。在大田试验中,采用随机区组设计,设两个重复,对CT9993-5-10-1-M × IR62266-42-6-2杂交而得 的144份水稻品系DH群体的产量及其主要农艺性状的数量性状位点(QTLs)进行定位。采用MAPMAKER/QTL 1．1软件进行区间定位并估计每个QTL的表型方差的百分比,以LOD阈值>2．4来确定一个QTL的有无.试验鉴 定出与6个水稻农艺性状如千粒重、单株产量、株高、穗长、粒宽、粒长宽比有关的23个QTLs,这些QTLs将有助于 提高水稻产量,并有利于了解水稻产量性状的遗传调控. 相似文献
5.
Mahipal Singh Kesawat Basanta Kumar Das G. R. Bhaganagare Vinay Sharma Manorama 《Journal of Crop Science and Biotechnology》2009,12(3):109-113
Formation of nodules on roots or in stems (in some cases) of leguminous plants is the unique ability of gram-negative bacteria,
Rhizobia, which converts atmospheric nitrogen into usable forms by the host plant. Lipopolysaccharide (LPS) is the outer membrane
component of the gram-negative bacteria, known to be an essential factor in host recognition, specificity, and initial infection
processes. In the present study, we extracted lipopolysaccharides from different rhizobial isolates by a modified phenol-water
method and partially characterized by polyacrylamide gel electrophoresis with silver staining. The results showed two separate
banding regions, LPS-I and LPS-II. The high molecular weight and electrophoretic mobility of LPS-I region resembles that of
lysozyme, used as a standard marker. The LPS-II region has a low molecular weight and electrophoretic mobility greater than
that of lysozyme. The LPS-II region was due to incomplete LPS, which either lacks the entire O-antigen repeating unit or contains
only one or two repeating units. The banding patterns of LPS vary among the different rhizobial isolates. Results revealed
that the type of LPS structure and banding regions greatly facilitate the further characterization of the LPS modifications
required for symbiosis. 相似文献
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Molecular markers: It’s application in crop improvement 总被引：1，自引：0，他引：1
Mahipal Singh Kesawat Basanta Das Kumar 《Journal of Crop Science and Biotechnology》2009,12(4):169-181
Over the past few decades, plant genomics research has been studied extensively bringing about a revolution in the field of
plant biotechnology. Molecular markers, useful for plant genome analysis, have now become an important tool in crop improvement.
The development and use of molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant
developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their
principles, methodologies and applications require careful consideration in choosing one or more of such methods. No molecular
markers are available yet that fulfill all requirements needed by researchers. In this article we attempt to review most of
the available DNA markers that can be routinely employed in various aspects of plant genome analysis such as characterization
of genetic variability, genome fingerprinting, genome mapping, gene localization, analysis of genome evolution, population
genetics, taxonomy, plant breeding, and diagnostics. The emerging patterns make up a unique feature of the analyzed individual
and are currently considered to be the ultimate tool for biological individualization. 相似文献
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