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1.
A. S. Timoshchenko V. F. Semikhov L. O. Nurminskaya P. N. Kharchenko 《Russian Agricultural Sciences》2014,40(1):1-4
During 1–7-day stages of ontogenesis (for example, in winter rye), biochemical changes in the aerial part of perennial grasses with adaptive Triticum-type proteins in the process of entering into the state of anabiosis were studied. It was shown that, at this point, biomass of the aerial part of plant was much higher than of the root system, and it accumulated raffinose, sucrose, glucose, and half-cystine. Synthesis of raffinose and accumulation of half-cystine continued until the stabilization of negative temperatures; as for sucrose and glucose, it ceased upon reaching the average temperature of the physiological zero, then the content of half-cystine and raffinose increased sharply, and the content of sucrose and glucose decreased at the same time. In the aerial parts of plants, fructose and phenylalanine were found, and their content increased upon reaching freezing temperatures. It was concluded that the polymerization of water-soluble proteins occurred in perennial grasses in a state of anabiosis. These proteins, along with raffinose, serve as cryoprotectants; sucrose and glucose are substrates for respiration; fructose is a substrate for respiration and protective metabolite; and phenylalanine is a substrate for the future synthesis of antioxidants. 相似文献
2.
ZS Perényi O Szenci PV Drion H Banga-Mboko NM Sousa B El Amiri JF Beckers 《Reproduction in domestic animals》2002,37(6):324-329
Pregnancy‐associated glycoproteins (PAGs) isolated from the placenta of various ruminant species are enzymatically inactive members of the aspartic proteinase family. The measurement of these proteins in the maternal blood can be a good indicator of the presence of a live embryo. As certain aspartic proteinases are present in biological fluids in physiological and pathological conditions at various concentrations, it was necessary to determine the specificity of three radioimmunoassay (RIA) systems currently used for the detection of PAG molecules. Commercially available members of the aspartic proteinase family like pepsinogen, pepsin, chymosin, rennet, cathepsin D and renin were tested in a wide concentration range (10 ng/ml – 1 mg/ml). Pepsinogen cross‐reacted in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 50 μg/ml and 500 μg/ml concentrations, respectively. In the presence of pepsin, cross‐reaction was observed in RIA 1, RIA 2 and RIA 3 over 1 mg/ml, 500 μg/ml and 1 mg/ml concentrations, respectively. Chymosin and rennet could cross‐react in RIA 2 and RIA 3, while renin and cathepsin D did not decrease the binding of the tracer to antisera more, than that of the minimal detection limit. As the plasma/serum concentrations of the examined aspartic proteinases reported in the literature were outside the concentration range where cross‐reaction was observed, it can be concluded that these RIA systems were specific for the detection of PAGs in biological fluids. 相似文献
3.
E. N. Baranova A. A. Gulevich V. Yu. Polyakov P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(4):236-238
The anatomic structure of cotyledons of clone 124 and cultivar Nadezhda during germination in various isosmotic sodium chloride and mannitol concentrations is compared. The possibility of using a quantitative method for revealing the character of salt tolerance in plants obtained by biotechnological methods is shown. 相似文献
4.
Yu. V. Aniskina A. N. Matveev E. I. Khor℉kov I. A. Shilov P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(5):295-297
A technology has been developed for genotyping spring rape cultivars on the basis of microsatellite analysis, which makes it possible to reliably distinguish cultivars, including those closely related, and it can be used for their passportization and certification, compiling genealogies, and protecting the intellectual property of breeders. 相似文献
5.
Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows 
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下载免费PDF全文 PV Ortega Serrano A Guzmán CG Hernández–Coronado H Castillo‐Juárez AM Rosales‐Torres 《Reproduction in domestic animals》2016,51(6):985-991
The vascular endothelial growth factor (VEGF) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR1 and VEGFR2, and two soluble receptors, sVEGFR1 and sVEGFR2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF, VEGFR1, VEGFR2, sVEGFR1 and sVEGFR2 in bovine preselected follicles (PRF) and post‐selected follicles (POF). The mRNA expression of these five genes in both granulosa cells (GC) and theca cells (TC) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR1 and sVEGFR2 in POF than in PRF (p < .05). Regarding the mRNA expression of total VEGF, VEGFR1 and VEGFR2, there was no difference between POF and PRF follicles (p > .05). Our results showed that the mRNA expression of VEGFR2 and sVEGFR1 was more abundant than the expression of VEGFR1 and sVEGFR2, while GC was the main source of mRNA for total VEGF. On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non‐dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR1 and sVEGFR2, which may favour VEGF binding with VEGFR2 and, hence, improve the follicular health and development. 相似文献
6.
A. S. Timoshchenko V. F. Semikhov P. N. Kharchenko L. O. Nurminskaya 《Russian Agricultural Sciences》2011,37(6):441-446
The state of overwintering cereals during their transition into anabiosis is studied. It is shown that by the time growth
processes associated with a decrease of temperature below physiological zero ceases, free amino acids are utilized, the quantitative
ratio of protein amino acids changes, and raffinose, sucrose, and phenylalanine accumulate in the aboveground part of the
plants. It is concluded that the transition of cereals to a state of anabiosis occurs upon maximum binding of intracellular
water by the protein-carbohydrateamino acid complex after stabilization of negative ambient temperatures. The results of this
work and a number of previous publications permitted formulating an idea about the structure of the physiological and biochemical
mechanism of adaptation to negative temperatures acquired by perennial cereals during evolution. 相似文献
7.
T. G. Satina Yu. V. Aniskina V. V. Karpachev I. A. Shilov P. N. Kharchenko 《Russian Agricultural Sciences》2010,36(1):12-14
Sixteen microsatellite loci suitable for identifying rapeseed varieties are established; 16 original primer pairs are proposed
for analyzing polymorphism of these microsatellite loci. Unique genetic profiles of 13 homebred and foreign-bred rapeseed
varieties are obtained. 相似文献
8.
D. V. Grishin V. P. Gudov O. V. Sergienko V. G. Lunin P. N. Kharchenko 《Russian Agricultural Sciences》2008,34(5):329-331
A genetic engineering construct is created which encodes a chimeric protein for nonviral transgenesis that includes the SSB DNA-binding domain and VirD2 nuclear localization signal. A method is developed for purifying the target protein. 相似文献
9.
E. K. Serenko V. N. Ovchinnikova L. V. Kurenina E. N. Baranova A. A. Gulevich A. N. Maisuryan P. N. Kharchenko 《Russian Agricultural Sciences》2009,35(4):223-226
Agrobacterial transformation of tomatoes by an expression vector containing the Fe-SOD gene is carried out. The transgenic
status of the transformants obtained is confirmed by PCR analysis. Differences are noted in the cell cycle and ultrastructure
of transgenic and nontransgenic plants under the effect of salt stress. 相似文献
10.
M. S. Gins V. K. Gins G. Yu. Rabinovich N. G. Kovalev V. A. Kharchenko 《Russian Agricultural Sciences》2009,35(5):313-315
The complex effect of multipurpose compost (MPC) on the system soil-associative microflora-plant, increasing the numbers of
soil microorganisms, their enzymatic activity, and NPK content and promoting an increase of celery yield by 1.5–2 times, is
revealed. In this case, the content of nitrates in organs is within the limits of the maximum allow concentration. 相似文献