Enzyme Immunoassays for the Determination of Ovine LH and FSH   总被引：2，自引：0，他引：2  

GM Peclaris  A Pappa  K Deligiannis  K Koutsotolis 《Reproduction in domestic animals》2003,38(5):367-372

The development of competitive enzyme immunoassays for ovine plasma LH (oLH) and FSH (oFSH) is described. Standards and plasma samples were preincubated with diluted antiserum to oLH or oFSH and the reacted solution (100 μl per well) was transferred to plates previously coated with oLH or oFSH, respectively. The second antibody used was anti‐rabbit IgG horseradish peroxidase. The measuring range was 0.39–50 ng/ml for each hormone and the 50% relative binding sensitivity was 9 ng/ml for oLH. The respective value for oFSH was 3.5 or 34 ng/ml with different hormone and antibody preparations used for the assay. The enzyme immunoassays were used to determine oLH and oFSH levels in plasma from ewes of two breeds during the oestrous cycle. The assays detected the first FSH surge coincident with the LH surge, the second FSH surge about 24 h later and the periodic fluctuations of FSH concentrations during the luteal phase of the oestrous cycle. These enzyme immunoassays are an efficient and economic alternative to the established radioimmunoassays (RIA) for oLH and oFSH.  相似文献   

Heat stress and effect of shade materials on hormonal and behavior response of dairy cattle: a review     

Reena Kamal  Triveni Dutt  Manjunath Patel  Amitava Dey  Panch Kishore Bharti  Poolangulam Chinnakkan Chandran 《Tropical animal health and production》2018,50(4):701-706

In tropical countries, at high temperatures, several physiological rearrangements occur in cows as they attempt to facilitate heat dissipation and/or reduce metabolic heat production. Following exposure to heat, cattle appear to acclimatize within 2–7 weeks. The failure of homeostasis at high temperatures may lead to reduced productivity or even death. The situation is even worse when humidity adds to high temperature. Livestock with elevated body temperature exhibit lower DMI and growth with less efficiency, reducing profitability for dairy farms in hot and humid climates. Shading of feed and water also offers production advantages. Although several elaborate methods for reducing heat stress in cows have been reported, simple shade materials appear to be the most cost-effective methods that are currently applicable to tropical developing countries. Different materials are being used to provide shade during warm weather. The shade material determines the microclimate—it should be light, strong, durable, weatherproof, good looking and a bad conductor of heat, and free from tendency to condense moisture inside. This review discusses various shade materials and their advantages and disadvantages in different situations.  相似文献   

Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow          下载免费PDF全文

Pradeep Jatav  Monika Sodhi  Ankita Sharma  Sandeep Mann  Amit Kishore  Umesh K Shandilya  Ashok K Mohanty  Ranjit S Kataria  Poonam Yadav  Preeti Verma  Surinder Kumar  Dhruba Malakar  Manishi Mukesh 《Animal Science Journal》2016,87(3):344-353

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Some characteristics of vaginal prolapse in Nepali buffaloes     

Sah SK  Nakao T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1213-1215

Prolapse of vagina is one of the important maternal abnormalities during pregnancy in cattle and buffaloes. A field investigation was carried out on 26 Murrah graded buffaloes to study clinical characteristics of vaginal prolapse in buffaloes in Nepal. Fifty-seven percent of the 26 buffaloes with vaginal prolapse were either heifers or in first lactation. Sixty-five percent of the cases were in seventh month of pregnancy or later. About three quarters of the cases occurred between June and October. Twelve cases (63%) of the 19 animals excluding 7 heifers had a history of vaginal prolapse in previous gestations. A half of the buffaloes were showing prolapse of the vagina even when they were in standing position and showing moderate or vigorous straining. After the conventional treatments, twenty-three buffaloes retained the replaced vagina and calved normally. One animal aborted although the vagina was retained. Two buffaloes had severest degree of vaginal prolapse complicated with edema, injury and cyanosis, and they did not respond to the treatment. The two buffaloes had frequently recurrent prolapse and subsequently died. Early detection and prompt treatment may be imperative to control the vaginal prolapse in buffaloes.  相似文献   

Haemorrhagic enteritis of turkeys     

KE ARZEY  GM CROSS 《Australian veterinary journal》1990,67(11):413-413

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The Control of Reactive Oxygen Species Influences Porcine Oocyte In Vitro Maturation          下载免费PDF全文

GM Alvarez  SA Morado  MP Soto  GC Dalvit  PD Cetica 《Reproduction in domestic animals》2015,50(2):200-205

The aim of this study was to examine the effect of varying intracellular reactive oxygen species (ROS) levels during oocyte in vitro maturation with enzymatic ROS production systems (xanthine + xanthine oxidase or xanthine + xanthine oxidase + catalase), scavenger systems (catalase or superoxide dismutase + catalase) or cysteine on porcine oocyte maturation. Oocyte ROS levels showed an increase when H₂O₂ or O₂?^‐ production systems were added to the culture medium (p < 0.05). On the other hand, the presence of ROS scavengers in the maturation medium did not modify oocyte ROS levels compared with the control after 48 h of maturation, but the addition of cysteine induced a decrease in oocyte ROS levels (p < 0.05). The ROS production systems used in this work did not modified the percentage of oocyte nuclear maturation, but increased the decondensation of sperm head (p < 0.05) and decreased the pronuclear formation (p < 0.05). In turn, the addition of O₂?^‐ and H₂O₂ scavenging systems during in vitro maturation did not modify the percentage of oocytes reaching metaphase II nor the oocytes with decondensed sperm head or pronuclei after fertilization. However, both parameters increased in the presence of cysteine (p < 0.05). The exogenous generation of O₂?^‐ and H₂O₂ during oocyte in vitro maturation would not affect nuclear maturation or later sperm penetration, but most of the spermatozoa cannot progress to form the pronuclei after fusion with the oocyte. The decrease in endogenous ROS levels by the addition of cysteine would improve pronuclear formation after sperm penetration.  相似文献   

Phosphofructokinase and Malate Dehydrogenase Participate in the In Vitro Maturation of Porcine Oocytes     

E Breininger  BE Vecchi Galenda  GM Alvarez  C Gutnisky  PD Cetica 《Reproduction in domestic animals》2014,49(6):1068-1073

Oocyte maturation depends on the metabolic activity of cumulus–oocyte complex (COC) that performs nutritive and regulatory functions during this process. In this work, the enzymes [phosphofructokinase (PFK) and malate dehydrogenase (MDH)] were tested to elucidate the metabolic profile of porcine COCs during the in vitro maturation (IVM). Enzymatic activity was expressed in U/COC and U/mg protein (specific activity) as mean ± SEM. In vitro maturation was performed with 2‐oxoglutarate (5, 10 and 20 mm ) or hydroxymalonate (30, 60 and 100 mm ) inhibitors of PFK and MDH, respectively. The PFK and MDH activities (U) remained constant during maturation. For PFK, the U were (2.48 ± 0.23) 10^?5 and (2.54 ± 0.32) 10^?5, and for MDH, the U were (4.72 ± 0.42) 10^?5 and (4.38 ± 0.25) 10^?5 for immature and in vitro matured COCs, respectively. The specific activities were significantly lower after IVM, for PFK (4.29 ± 0.48) 10^?3 and (0.94 ± 0.12) 10^?3, and for MDH (9.08 ± 0.93) 10^?3 and (1.89 ± 0.10) 10^?3 for immature and in vitro matured COCs, respectively. In vitro maturation percentages and enzymatic activity diminished with 20 mm 2‐oxoglutarate or 60 mm hydroxymalonate (p < 0.05). Viability was not affected by any concentration of the inhibitors evaluated. The U remained unchanged during IVM; however, the increase in the total protein content per COC provoked a decrease in the specific activity of both enzymes. Phosphofructokinase and MDH necessary for oocyte IVM would be already present in the immature oocyte. The presence of inhibitors of these enzymes impairs the meiotic maturation. Therefore, the participation of these enzymes in the energy metabolism of the porcine oocyte during IVM is confirmed in this study.  相似文献   

Role of micronutrients on quantitative traits and prospects of its accumulation in linseed (Linum usitatissimum L.)     

Anu Rastogi  Brij Kishore Mishra  Munna Singh  Ritu Mishra 《Archives of Agronomy and Soil Science》2013,59(10):1389-1409

The adequate growth of plants can be achieved only by a balanced ratio of biologically active nutrients in soil along with its readily availability. Linseed seems to be an advantageous secondary oilseed crop widely used in industrial purposes, which can serve additional purpose of phytoextraction of heavily infested soils with heavy metals. In this study, we have made an effort to find out the optimum dose of heavy metals necessary as micronutrients like copper (Cu), iron (Fe) and zinc (Zn) for the enhancement of linseed yield along with the distribution of heavy metals in different plant parts. Quantitative enhancement in different traits of linseed plant due to Cu, Fe and Zn fertilization especially for capsules per plant after maturity suggested positive association between heavy metal supply and morphological traits, which indirectly enhanced seed yield. There was a positive correlation between Zn supply and seed yield. In the treatments with Cu and Fe fertilization, seed yield was positively correlated to plant height, capsules per plant and seeds per five capsules. According to this study, it is concluded that linseed has the ability to accumulate majorly Fe followed by Cu and Zn.  相似文献   

The resistance of meat chickens vaccinated by aerosol with a live V4 Newcastle disease virus vaccine in the field to challenge with a velogenic Newcastle disease virus     

IG BELL  PJ NICHOLLS  C. NORMAN  AINI IDERIS†  GM CROSS† 《Australian veterinary journal》1991,68(3):97-101

Meat chickens housed on a commercial broiler farm in Australia were vaccinated once at 10 to 11 days-of-age by aerosol with live V4 Newcastle disease virus (NDV) vaccine. Groups of vaccinated and unvaccinated birds were flown to Malaysia, where they were challenged with a virulent strain of NDV. Survival rates in vaccinated chickens challenged 7, 14, 21 or 31 d after vaccination were 0.47, 0.77, 0.97 and 0.92, respectively. All unvaccinated chickens died due to Newcastle disease (ND) following challenge. Chickens in Australia and Malaysia were bled and the serums tested for haemagglutination-inhibiting (HI) antibody to NDV. Many vaccinated birds with no detectable antibody, and all birds with a log2 titre of 2 or greater, survived challenge. The results showed that this V4 vaccine induced protective immunity in a significant proportion of chickens within 7 d of mass aerosol vaccination. This early immunity occurred in the absence of detectable circulating HI antibody. Non-HI antibody mediated immunity continued to provide protection up to 31 d after vaccination. Almost all vaccinated birds were protected within 3 w of vaccination. It is concluded that the V4 vaccine is efficacious and could be useful during an outbreak of virulent ND in Australia.  相似文献   

The retrospective diagnosis of a second outbreak of equine morbillivirus infection   总被引：5，自引：0，他引：5  

PT HOOPER  AR GOULD  GM RUSSELL  JA KATTENBELT  G. MITCHELL 《Australian veterinary journal》1996,74(3):244-245

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