Soil Phosphorus and Potassium Mapping Using a Spatial Correlation Model Incorporating Terrain Slope Gradient   总被引：1，自引：0，他引：1  

Kozar  Brian  Lawrence  Rick  Long  Dan S. 《Precision Agriculture》2002,3(4):407-417

Variable-rate fertilizer application requires knowledge of the spatial distribution of soil nutrients within fields. Grid soil sampling might be used for acquiring this information, but is often too expensive for resolving spatial patterns in soil nutrients at the scale of precision fertilizer application. The objective of this study was to determine whether grid sampling efficiency can be improved using cokriging estimates with slope gradient as a secondary variable, which is easily obtained from high-resolution digital elevation models. Soils in two northern Montana wheat fields were sampled at the nodes of a 100-m diagonal grid. Soil test phosphorus and potassium maps were constructed with kriging and cokriging. Co-kriging uses the spatial correlation between two variables to predict for the less intensively sampled variable of interest, often with less estimation error than a univariate method such as kriging. The average estimation variance for cokriging compared to kriging was reduced for all values of the correlation considered. The additional complexity of cokriging might be justified provided a secondary variable exists that is spatially cross correlated with the primary variable of interest.  相似文献   

Germline transmission of exogenous genes in the chicken   总被引：7，自引：0，他引：7  

R A Bosselman  R Y Hsu  T Boggs  S Hu  J Bruszewski  S Ou  L Kozar  F Martin  C Green  F Jacobsen 《Science (New York, N.Y.)》1989,243(4890):533-535

Difficulties associated with in vitro manipulation and culture of the early chicken embryo have restricted generation of transgenic chickens to approaches that use replication-competent retroviruses. The need to produce transgenic chickens in the absence of replicating virus prompted development of a new method of gene transfer into the chicken. Microinjection of the replication-defective reticuloendotheliosis virus (REV) vector ME111 beneath unincubated chicken embryo blastoderms results in infection of germline stem cells. This vector contains genetic information exogenous to the chicken genome, including both the herpes simplex virus type 1 thymidine kinase gene and the Tn5 neomycin phosphotransferase gene. About 8 percent of male birds hatched from injected embryos contained vector DNA in their semen. All four positive males tested passed vector sequences onto their progeny. Analysis of G1 offspring showed that gonads of G0 male birds were mosaic with respect to insertion of vector provirus. Thus, primordial germ cells present in the unincubated chicken embryo blastoderm are susceptible to infection by defective REV vectors.  相似文献