Hypokalaemic episodic polymyopathy in cats fed a vegetarian diet     

A. LEON  SAF BAIN  WR LEVICK 《Australian veterinary journal》1992,69(10):249-254

A previously undocumented hypokalaemic condition with a cyclical nature, comprising acute bouts of polymyopathy followed by spontaneous recoveries, is described in the cat. Cats being fed a high protein vegetarian diet developed recurrent episodes of polymyopathy, characterised by ventroflexion of the head and neck, stiff forelimb gait, lateral head-resting and generalised muscle weakness. Plasma potassium concentrations (mean +/- standard deviation) were reduced from 3.28 +/- 0.33 mmol/l at the beginning of the experiment to 2.45 +/- 0.24 mmol/l during bouts of myopathy. This hypokalaemia was associated with increased creatine kinase activities indicative of muscle damage, and decreased urinary potassium concentrations, and was caused by insufficient dietary potassium. Cats that received the same diet supplemented with potassium did not develop hypokalaemic polymyopathy. Spontaneous recoveries of affected cats were not associated consistently with increases in plasma potassium concentrations. Plasma taurine concentrations decreased and glutamic acid increased markedly in all cats fed the experimental diet. There was no evidence of thiamin deficiency associated with the high glutamic acid intake. Veterinarians should be aware that hypokalaemic cats, and in particular those on potassium-deficient diets, may show cyclical disease with episodes of polymyopathy recurring after periods of spontaneous clinical recovery. This condition in cats may be a useful animal model for familial hypokalaemic periodic paralysis in humans.  相似文献   

Central nervous system signs in chickens caused by a new avian reovirus strain: a pathogenesis study     

Van de Zande S  Kuhn EM 《Veterinary microbiology》2007,120(1-2):42-49

The present study describes the pathogenesis of infection of chicks with a new avian reovirus strain, belonging to the so-called enteric reovirus strains (ERS) that is capable of causing central nervous system signs in SPF white leghorns. After intramuscular (IM) or oral inoculation birds were either observed for clinical signs or sacrificed for macroscopic, histological and virological examination for 21 days. Virus isolation was performed on the brain, leg muscle, hock joint, liver and spleen. For the detection of viral antigen the immunohistochemistry (IHC) technique was performed on the caudal part of the cerebrum, spinal cord including spinal ganglia and right N. Ischiadicus. High mortality (79% in 7 days) was seen in birds that were inoculated IM. Survivors were depressed and stayed small until the end of the experiment. One bird had tremor and showed torticollis at 9 days after IM inoculation. Birds that were inoculated orally were depressed from day 4 and stayed small until the end of the experiment. One bird showed a torticollis at 10 days after inoculation. After both IM and oral inoculation ERS was isolated from the brain between 3 and 10 days after inoculation. Other examined organs were positive for virus isolation from day 1 or 5 until day 21. IHC revealed viral antigen positive cells in the Plexus chorioideus (plexus epithelial cells or cells within the underlying connective tissue) and in a spinal ganglion. The results indicate that the pathogenesis of ERS infection in chickens bears some resemblance with that of the mammalian reoviruses serotype 1 in mice.  相似文献   

Evaluation of a novel moving threshold gating strategy for assessment of reticulated platelets in dogs using the ADVIA 2120 analyzer     

Anja Kuhn  Kristina Weiler  Graham Gibbs  Gottfried Prechtl  Natali Bauer  Andreas Moritz 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(1):11-21

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Evidence of sedimentation inequality along riparian areas colonised by Impatiens glandulifera (Himalayan balsam)     

P Greenwood  A C Gange  N J Kuhn 《Weed Research》2020,60(1):26-36

Soil loss from riparian areas supporting the annual invasive weed, Impatiens glandulifera (Himalayan balsam), was measured and compared with equivalent values recorded at nearby, topographically similar areas supporting perennial vegetation over a cumulative seven-year period, along sections of two separate river systems; one in Switzerland, and one in the UK. Soil loss from colonised locations was significantly greater than from reference locations in four of the seven measurement periods. Despite contrasting results, standard deviations, based on soil losses and gains, were predominantly higher for colonised areas at both rivers over most monitoring periods. These findings indicated that areas colonised by Himalayan balsam experience higher sediment flux in comparison with areas free of invasion. Here, we test those original interpretations by reinterrogating the datasets using a more robust analysis of inequality. Nine datasets were tested, five of which (i.e. 56%) showed that sediment flux was significantly greater at Himalayan balsam-invaded areas than at reference areas. Three datasets showed no difference in sediment flux between invaded and reference areas (33%), and one (11%) showed higher sediment flux at reference areas. Most results uphold our original interpretations and support our hypothesis that hydrochory probably dictates where colonisation initially occurs, by depositing Himalayan balsam seeds in slack or depressional areas along river margins. Once Himalayan balsam becomes established and sufficient perennial vegetation is displaced, seasonal die-off and depleted vegetation cover may increase the risk that some areas will experience significantly higher sediment flux.  相似文献   

The adoption of annual subsoiling as conservation tillage in dryland maize and wheat cultivation in northern China   总被引：9，自引：0，他引：9  

He Jin  Li Hongwen  Wang Xiaoyan  A.D. McHugh  Li Wenying  Gao Huanwen  N.J. Kuhn 《Soil & Tillage Research》2007,94(2):493-502

Soil compaction caused by random traffic or repetitive tillage has been shown to reduce water use efficiency, and thus crop yield due to reduced porosity, decreased water infiltration and availability of nutrients. Conservation tillage coupled with subsoiling in northern China is widely believed to reduce soil compaction, which was created after many years of no-till. However, limited research has been conducted on the most effective time interval for subsoiling, under conservation tillage. Data from conservation tillage demonstration sites operating for 10 years in northern China were used to conduct a comparative study of subsoiling interval under conservation tillage. Three modes of traditional tillage, subsoiling with soil cover and no-till with soil cover were compared using 10 years of soil bulk density, water content, yield and water use efficiency data. Cost benefit analysis was conducted on subsoiling time interval under conservation tillage. Yield and power consumption were assessed by based on the use of a single pass combine subsoiler and planter. Annual subsoiling was effective in reducing bulk density by only 4.9% compared with no-till treatments on the silty loam soils of the Loess plateau, but provided no extra benefit in terms of soil water loss, yield increase or water utilization. With the exception of bulk density, no-till and subsoiling with cover were vastly superior in increasing water use (+10.5%) efficiency and yield (+12.9%) compared to traditional tillage methods. Four years of no-till followed by one subsoiling reduced mechanical inputs by 62%, providing an economic benefit of 49% for maize and 209% for wheat production compared to traditional tillage. Annual subsoiling reduced inputs by 25% with an increased economic benefit of 23% for maize and 135% for wheat production. Yield and power consumption was improved by 5% and 20%, respectively, by combining subsoiling with the planting operation in one pass compared with multipass operations of subsoiling and planting. A key conclusion from this is that annual subsoiling in dryland areas of northern China is uneconomical and unwarranted. Four years of no-till operations followed by 1 year subsoiling provided some relief from accumulated soil compaction. However, minimum soil disturbance and maximum soil cover are key elements of no-till for saving water and improving yields. Improved yields and reduced farm power consumption could provide a significant base on which to promote combined planter and subsoiling operations throughout northern China. Further research is required to develop a better understanding of the linkages between conservation tillage, soil quality and yield, aimed at designing most appropriate conservation tillage schemes.  相似文献   

Antibody response to Raboral VR-G® oral rabies vaccine in captive and free-ranging black-backed jackals (Canis mesomelas)     

Katja N. Koeppel  Peter Geertsma  Brian F. Kuhn  Ockert L. van Schalkwyk  Peter N. Thompson 《The Onderstepoort journal of veterinary research》2022,89(1)

Rabies is a zoonotic disease that remains endemic in large parts of southern Africa because of its persistence in wildlife and domestic dog vectors. The black-backed jackals (Canis mesomelas) is primarily the wildlife vector responsible for rabies outbreaks in northern parts of South Africa. Two trials were carried out to investigate antibody responses to the oral rabies vaccine Raboral V-RG® in black-backed jackals under captive and free-ranging conditions. In captive jackals 10/12 (83%; 95% confidence interval [CI]: 52% – 98%), seroconverted after single oral vaccination. Nine captive jackals had protective antibody titres (> 0.5 IU/mL) at 4 weeks (median: 2.1 IU/mL; inter quartile range [IQR]: 0.6–5.7) and 10 jackals had at 12 weeks (median: 3.5 IU/mL; IQR: 1.5–8.3) and three maintained antibody titres for up to 48 weeks (median: 3.4 IU/mL; IQR: 2.0–6.3). Four sites were baited with Raboral V-RG® vaccine for wild jackals, using fishmeal polymer and chicken heads. Baits were distributed by hand or from vehicle at three sites in north-eastern South Africa, with an average baiting density of 4.4 baits/km² and at one site in central South Africa, at 0.12 baits/km². This resulted in protective antibody titres in 3/11 jackals (27%; 95% Cl: 6–61) trapped between 3 and 12 months after baiting in north-eastern South Africa, compared with 4/7 jackals (57%; 95% Cl: 18–90) trapped after 3–18 months in central South Africa. This study shows the potential utility of oral rabies vaccination for the control of wildlife-associated rabies in north-eastern and central South Africa, but extensive studies with wider distribution of bait are needed to assess its potential impact on rabies control in wild jackals.  相似文献   

Molecular analysis of the Arbuscular mycorrhiza symbiosis     

Philipp Franken  Natalia Requena  Britta Bütehorn  Franziska Krajinski  Gerrit Kuhn  Laurence Lapopin 《Archives of Agronomy and Soil Science》2013,59(4):271-286

The molecular analysis of the arbuscular mycorrhizal (AM) symbiosis started at the beginning of this decade. The paper summarises the work of the ‘Laboratorium für Molekulare Genetik arbuskulärer Mykorrhiza’ at the MPI für terrestrische Mikrobiologie in Marburg on three major topics. Firstly, the plant response to the mycorrhizal colonisation was analysed using both, targeted and non‐targeted approaches. As an example, the localisation of the gst1 mRNA in potato mycorrhiza is shown. Second, molecular techniques were established to analyse gene expression of the fungal partner of the symbiosis. We present a differential RNA display analysis of spore germination in two AM fungi and the cloning of a gene from Glomus mosseae that shows expression at all stages of the fungal life cycle. In the last part, we introduce the work we are carrying out with a new root endophytic fungus, Piriformospora indica. Infection experiments on maize showed that despite the fungus performs as a root necrotroph, it has a positive effect on plant growth.  相似文献   

The effect of prostaglandin F2alpha and clinical experiences with its analog "Estrumate", especially in cattle anestrus     

W Leidl  H Bostedt  R Stolla  W Schefels  A Kuhn 《Berliner und Münchener tier?rztliche Wochenschrift》1977,90(16):309-314

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Kiefernstarkholzzucht     

Wilhelm Kuhn 《Forstwissenschaftliches Centralblatt》1918,40(8-9):307-321

Ohne Zusammenfassung  相似文献   

Development of a marker assisted selection program for cacao     

Schnell RJ  Kuhn DN  Brown JS  Olano CT  Phillips-Mora W  Amores FM  Motamayor JC 《Phytopathology》2007,97(12):1664-1669

ABSTRACT Production of cacao in tropical America has been severely affected by fungal pathogens causing diseases known as witches' broom (WB, caused by Moniliophthora perniciosa), frosty pod (FP, caused by M. roreri) and black pod (BP, caused by Phytophthora spp.). BP is pan-tropical and causes losses in all producing areas. WB is found in South America and parts of the Caribbean, while FP is found in Central America and parts of South America. Together, these diseases were responsible for over 700 million US dollars in losses in 2001 (4). Commercial cacao production in West Africa and South Asia are not yet affected by WB and FP, but cacao grown in these regions is susceptible to both. With the goal of providing new disease resistant cultivars the USDA-ARS and Mars, Inc. have developed a marker assisted selection (MAS) program. Quantitative trait loci have been identified for resistance to WB, FP, and BP. The potential usefulness of these markers in identifying resistant individuals has been confirmed in an experimental F(1) family in Ecuador.  相似文献