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1.
Singh Sultan Kushwaha Badri Prasad Maity Subendu Bikas Singh Krishan Kunwar Das Nityanand 《Tropical animal health and production》2015,47(1):213-220
Fifteen Bhadawari buffalo heifers of 207?±?9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (N) balance, growth rate, blood metabolites, and puberty. All animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 Mcal/kg) and different protein levels (14.3–22 %). Animals of standard-protein group (SPG) were offered protein and energy as per requirement, while animals of low-protein group (LPG) and high-protein group (HPG) were fed 20 % less and 20 % more protein, respectively, than SPG. Feed dry matter (DM) and metabolizable energy (ME) intake (% body wt. and g/kg w0.75) were similar for all three diets; however, the crude protein (CP) and digestible crude protein (DCP) intake on percent body weight and per kilogram metabolic weight was higher (P?<?0.05) in HPG than in SPG or LPG. Digestibility of CP, cellulose, and hemicellulose was higher (P?<?0.05) in HPG versus LPG. Fecal N excretion was similar, while urinary N excretion was highest (P?<?0.05) in HPG (74.83 g/day) compared with SPG (50.03 g/day) and LPG (47.88 g/day), which resulted in lower N retention in HPG than in the other dietary groups. Level of dietary N had no effect on blood metabolites viz. glucose, urea, and N. Digestible energy (DE) and ME contents of diets were identical, while DCP contents were higher (P?<?0.05) in HPG than in LPG. Feed and nutrient (CP and ME) conversion efficiency to produce a unit kilogram weight gain was identical among the dietary groups. Dietary protein level had no effect on the heifer’s weight and age at puberty. The mean growth rate of heifers at 240 days was higher (P?>?0.05) in SPG (330.8 g/day) than in LPG (296.7 g/day), while the animals gained more weight in January to March months and the lowest weight in May to July months. Protein level had no effect on conception rate of heifers. Results revealed that 20 % higher or less protein than the ICAR requirement had no significant (P?>?0.05) on feed intake, nutrient conversion efficiency for weight gain, heifer growth, and puberty; however, 20 % more protein increased urinary N loss.
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Kunwar P. Singh Amrita Malik Sarita Sinha Vinod K. Singh 《Water, air, and soil pollution》2007,185(1-4):79-93
Multi-block (heavy metals, pesticides, physico-chemical parameters) data set pertaining to the soils of alluvium region in Indo-Gangetic plains was analyzed using principal component analysis (PCA) and multiple factor analysis (MFA) methods to delineate the contaminated sites and to identify the possible contamination sources in the study region. In normal PCA, the first three factors were dominated mainly by heavy metals, pesticides and physico-chemical variables, respectively, thus identifying samples/sites contaminated with these. The MFA results, due to its unique weighting scheme of variables of different blocks extracted, to more realistic information about the spatial distribution of samples and relationships among the variables. MFA minimized the influence of variables of one single block on the first few components, allowing variables of all blocks equally to share the common MFA space. This resulted in delineating the sites/regions contaminated with variables (Al, Co, Cu, Mn, Ni, Pb, V, Na, SO4, aldrin, lindane, HCB, HCH, DDT, and endosulfan) of all the blocks, rather than by particular block variables as in case of normal PCA, where, the variables of single block dominate the first factors, suppressing other block variables. MFA which can be considered as a method for standardization of the multi-block variables was successfully applied to the three block data set of soils. 相似文献
3.
Yonas Kefialew Sanju Kunwar Dawit Abate Amare Ayalew James Colee Laura Ritchie Stephen M. Olson Mathews L. Paret 《Journal of General Plant Pathology》2018,84(4):284-295
This study evaluated the direct antifungal activity of tiadinil [N-(3-chloro-4-methylphenyl)-4-methyl-1,2,3-thiadiazole-5-carboxamide], a systemic acquired resistance (SAR) inducer and two formulations of thymol (thymol I and thymol II) against Stagonosporopsis citrulli, the causal agent of gummy stem blight (GSB) disease of watermelon. Tiadinil, thymol I and thymol II completely inhibited the mycelial growth of S. citrulli in vitro at ≥?100 ppm. Conidial germination and germ tube elongation were completely inhibited by tiadinil at ≥?2000 ppm and by thymol-based formulations at ≥?100 ppm. A single foliar application of tiadinil at ≥?10 ppm or a single application of thymol I and II at ≥?1 ppm, 48 h before or after pathogen inoculation, significantly reduced disease severity of watermelon seedlings inoculated with 105/ml conidial suspension of S. citrulli, compared to respective nontreated controls. Plants treated with foliar application of tiadinil at ≥?1000 ppm before pathogen inoculation had significantly lower disease severity than plants that received an equivalent drench application. The efficacy of foliar application of tiadinil was affected by concentration and frequency of application. The study suggests direct antifungal activity of tiadinil, indicating a new mode of action of tiadinil against GSB disease of watermelon. The study also demonstrated direct antifungal action of thymol, a formulated active compound of essential oils, against S. citrulli and GSB disease of watermelon. 相似文献
4.
Pridgeon JW Klesius PH Mu X Carter D Fleming K Xu D Srivastava K Reddy G 《Veterinary microbiology》2011,152(1-2):117-125
In 2009, a disease outbreak caused by Aeromonas hydrophila occurred in 48 catfish farms in West Alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. Virulence studies have revealed that the 2009 isolates of A. hydrophila are at least 200-fold more virulent than a 1998 Alabama isolate AL98-C1B. However, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of A. hydrophila. To understand the genetic differences between the highly virulent 2009 isolates and the less virulent AL98-C1B at molecular level, PCR-select bacterial genome subtractive hybridization was used in this study. A total of 96 clones were selected from the subtractive genomic DNA library. Sequencing results revealed that the 96 clones represented 64 unique A. hydrophila sequences. Of the 64 sequences, three (hypothetical protein XAUC_13870, structural toxin protein RtxA, and putative methyltransferase) were confirmed to be present in the three virulent 2009 Alabama isolates but absent in the less virulent AL98-C1B. Using genomic DNAs from nine field isolates of A. hydrophila with different virulence as templates, two sequences (hypothetical protein XAUC_13870 and putative methyltransferase) were found to be only present in highly virulent A. hydrophila isolates, but absent in avirulent isolates. 相似文献
5.
Ronald McKnight DVM Kunwar K. Srivastava DVM PhD 《Veterinary radiology & ultrasound》1995,36(2):111-111
6.
In a field experiment, the influence of several rootstocks of citrus, viz., Srinagar mandarin (C. reticulta Blanco), Malta common (C. sinensis Osbeck), Jambhiri (C. lambhiri Lush), Kagzi kalan (C. aurantifolia Swingle), Rangpur lime (C. limonia Osbeck), Carrizo citrange (bigeneric hybrid), Baduvapuli (C. pennivesiculata Tanaka), Cleopatra (C. reticulata Blanco), Troyer citrange (bigeneric hybrid) and Hill lemon (C. limon Barm f.) on mineral composition of Srinagar mandarin (C. reticulata Blanco) leaves was studied. The results showed a wide and varying influence of rootstocks on mineral composition in scion leaves due to their differential ability to absorb nutrients and to translocate to scion leaves. Malta and mandarin rootstocks were generally associated with less N, Ca and Mg concentrations in scion leaves than rough lemon and citranges rootstocks. Rangpur lime followed by other mandarins resulted in relatively low P concentration whereas malta and citranges caused high P concentration as compared to other stocks. Considering the influence on K concentration, plants on malta rootstocks exhibited high level whereas it was low in plants grown on citranges. 相似文献
7.
Nanjundan Joghee Aravind Jayaraman Radhamani Jalli Singh Kunwar Harendra Kumar Arun Thakur Ajay Kumar Singh Karnal Meena Kailash Narayan Tyagi Rishi Kumar Singh Dhiraj 《Genetic Resources and Crop Evolution》2022,69(1):145-162
Genetic Resources and Crop Evolution - Indian mustard [Brassica juncea (L.) Czern.] is a major edible oil crop of India. The Indian Council of Agricultural Research—National Bureau of Plant... 相似文献
8.
Pridgeon JW Aksoy M Klesius PH Li Y Mu X Srivastava K Reddy G 《Veterinary immunology and immunopathology》2011,144(1-2):111-119
To understand the molecular mechanisms involved in response of Nile tilapia (Oreochromis niloticus) to bacterial infection, suppression subtractive cDNA hybridization technique was used to identify upregulated genes in the posterior kidney of Nile tilapia at 6h post infection with Aeromonas hydrophila. A total of 31 unique expressed sequence tags (ESTs) were identified from 192 clones of the subtractive cDNA library. Quantitative PCR revealed that nine of the 31 ESTs were significantly (p<0.05) upregulated in Nile tilapia at 6h post infection with A. hydrophila at an injection dose of 10(5)CFU per fish (≈ 20% mortality). Of the nine upregulated genes, four were also significantly (p<0.05) induced in Nile tilapia at 6h post infection with A. hydrophila at an injection dose of 10(6)CFU per fish (≈ 60% mortality). Of the four genes induced by A. hydrophila at both injection doses, three were also significantly (p<0.05) upregulated in Nile tilapia at 6h post infection with Streptococcus iniae at doses of 10(6) and at 10(5)CFU per fish (≈ 70% and ≈ 30% mortality, respectively). The three genes induced by both bacteria included EST 2A05 (similar to adenylate kinase domain containing protein 1), EST 2G11 (unknown protein, shared similarity with Salmo salar IgH locus B genomic sequence with e value of 0.02), and EST 2H04 (unknown protein). Significant upregulation of these genes in Nile tilapia following bacterial infections suggested that they might play important roles in host response to infections of A. hydrophila and S. iniae. 相似文献
9.
Precision Agriculture - Nutrient assessment of plants, a key aspect of agricultural crop management and varietal development programs, traditionally is time demanding and labor-intensive. This... 相似文献
10.
Sanju Kunwar Yun-Che Hsu Shu-Fen Lu Jaw-Fen Wang Jeffrey B. Jones Samuel Hutton Mathews Paret Peter Hanson 《Plant Breeding》2020,139(2):389-401
Bacterial wilt of tomato caused by Ralstonia solanacearum species complex (RSSC) causes substantial yield losses in the tropics and subtropics. Disease management options by chemicals are limited, and host resistance is the cheapest and easiest means of control. However, sources of bacterial wilt resistance in tomato are limited. The disease often coincides with higher temperatures in the tropics, and resistance sources that are more heat stable are particularly valuable for breeding of tropically adapted tomato cultivars. The objectives of this study were to identify tomato accessions that demonstrate relatively high bacterial wilt resistance under high temperatures and to identify accessions that may possess QTLs other than Bwr-6 and Bwr-12 (two major disease resistance QTLs against bacterial wilt), which could be exploited in future breeding. Sixty-seven tomato entries reported as bacterial wilt resistant were evaluated in a greenhouse against one strain each of phylotype I (Pss4) and phylotype IIB (Pss1632) of the RSSC (average temperature ≥29°C). Of those, five and 19 were homozygous for Bwr-6 and Bwr-12, respectively, and six were homozygous for both QTLs. Bwr-12 contributed to resistance against phylotype I strain but not against the phylotype II strain. Bwr-6 contributed to resistance against both phylotype strains. Entries with both QTLs as a group performed relatively better against the phylotype I strain. Entry “94T765-24-79”, which lacked Bwr-6 and Bwr-12, demonstrated relatively high resistance against the phylotype II strain and may carry new QTL/s. As new bacterial wilt resistance QTLs are mapped and markers designed, pyramiding multiple bacterial wilt resistance QTLs into new varieties should be straightforward, thereby increasing the chances of obtaining stable resistance. 相似文献