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自1974年发现木豆雄性不育株以来,国际上不断对木豆核雄性不育(GMS)和核质互作雄性不育(CGMS)基因及其种质资源进行研究和挖掘,最终实现了木豆杂种优势的成功利用。本文系统总结了过去30多年来,国际上木豆杂种优势机理、表现及授粉方式等基础研究,GMS、CGMS不育系及其配套制种体系研究,杂种优势强度研究,以及木豆杂交种生产技术研究进展。提出了木豆杂种优势利用的具体研究方向和展望。 相似文献
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Loftus BJ Fung E Roncaglia P Rowley D Amedeo P Bruno D Vamathevan J Miranda M Anderson IJ Fraser JA Allen JE Bosdet IE Brent MR Chiu R Doering TL Donlin MJ D'Souza CA Fox DS Grinberg V Fu J Fukushima M Haas BJ Huang JC Janbon G Jones SJ Koo HL Krzywinski MI Kwon-Chung JK Lengeler KB Maiti R Marra MA Marra RE Mathewson CA Mitchell TG Pertea M Riggs FR Salzberg SL Schein JE Shvartsbeyn A Shin H Shumway M Specht CA Suh BB Tenney A Utterback TR Wickes BL Wortman JR Wye NH Kronstad JW Lodge JK 《Science (New York, N.Y.)》2005,307(5713):1321-1324
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ST Rolim Filho HFL Ribeiro GMF de Camargo DF Cardoso RR Aspilcueta‐Borquis H Tonhati KB Nunes WG Vale EM Barbosa KC de Sousa 《Reproduction in domestic animals》2013,48(5):705-709
The osteopontin gene may influence the fertility of water buffaloes because it is a protein present in sperm. The aim of this work was to identify polymorphisms in this gene and associate them with fertility parameters of animals kept under extensive grazing. A total of 306 male buffaloes older than 18 months, from two farms, one in the state of Amapá and the other in the state of Pará, Brazil were used in the study. Seven SNPs were identified in the regions studied. The polymorphisms were in gene positions 1478, 1513 and 1611 in the region 5′upstrem and positions 6690, 6737, 6925 and 6952 in the region amplified in intron 5. The SNPs were associated with the traits, namely scrotal circumference, scrotal volume, sperm motility, sperm concentration and sperm pathology. There were significant SNPs (p < 0.05) for all the traits. The SNP 6690 was significant for scrotal circumference, sperm concentration, sperm motility and sperm pathology and the SNP 6737 for scrotal volume. The genotype AA of SNP 6690 presented the highest averages for scrotal circumference, sperm concentration and motility and the lowest total number of sperm pathologies. For the scrotal volume trait, the animals with the largest volume were correlated with the presence of the genotype GG of SNP 6737. These results indicate a significance of the osteopontin gene as it seems to exert a substantial influence on the semen production traits of male buffaloes. 相似文献
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Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection 
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下载免费PDF全文 WY Lee MH Park KW Kim H Song KB Kim CS Lee NK Kim JK Park BC Yang KB Oh GS Im HJ Chung 《Reproduction in domestic animals》2017,52(1):16-23
Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous‐specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non‐oestrous and controlled internal drug release (CIDR)‐induced oestrous‐stage mucus proteins were purified and subjected to surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI‐TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor‐interacting protein 1 (GRIP1) showed a twofold increase during the CIDR‐induced oestrous stage compared to the levels in non‐oestrous stage in bovine cervical mucus. The RT‐PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non‐oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle. 相似文献
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