Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays   总被引：2，自引：0，他引：2  

C. Calderon ‡  E. Ward  J. Freeman  S. J. Foster  H. A. McCartney † 《Plant pathology》2002,51(3):303-310

The potential use of DNA-based methods for detecting airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae , both damaging pathogens of oilseed rape, was investigated. A method for purifying DNA from spores collected using Hirst-type spore samplers and detecting it using polymerase chain reaction (PCR) assays is described. For both pathogens, the sensitivities of the DNA assays were similar for spore-trap samples and pure spore suspensions. As few as 10 spores of L. maculans or P. brassicae could be detected by PCR and spores of both species could be detected against a background of spores of six other species. The method successfully detected spores of P. brassicae collected using spore traps in oilseed rape crops that were infected with P. brassicae. Leptosphaeria maculans spores were detected using spore traps on open ground close to L. maculans -infected oilseed rape stems. The potential use of PCR detection of airborne inoculum in forecasting the diseases caused by these pathogens is discussed.  相似文献   

Comparison of recovery times and complication rates between a modified slanted slot and the standard ventral slot for the treatment of cervical disc disease in 20 dogs     

McCartney W 《The Journal of small animal practice》2007,48(9):498-501

OBJECTIVES: To compare the recovery times and complication rates between the standard ventral slot and the modified slanted ventral slot for the treatment of cervical disc disease in dogs with the same neurological grade. METHODS: A retrospective analysis of an equal number of cervical disc cases for each procedure was carried out specifically to determine the recovery times and complication rates. To allow for meaningful comparison only dogs that had the same neurological grade were selected. Outcomes were determined by time to recover and the resolution of symptoms and complications were graded in terms of severity. RESULTS: The standard ventral slot cases had a higher complication rate than the modified slanted slot cases with one case dying in the postoperative period. However, the modified slanted slot cases on average had a marginally slower recovery time than standard ventral slot cases. CLINICAL SIGNIFICANCE: Ventral decompression using the modified slanted slot instead of the standard ventral slot will allow for spinal decompression with potentially less destabilisation, and incur a similar incidence of complications and rate of recovery.  相似文献   

Retracted: Effect of Post‐Thaw Storage Time on Motility and Fertility of Cryopreserved Beluga Sturgeon (Huso huso) Sperm          下载免费PDF全文

MS Aramli  RM Nazari  MR Gharibi 《Reproduction in domestic animals》2015,50(2):349-352

The aim of this study was to test the influence of post ‐ thaw storage time on the duration of sperm motility, percentage of motile sperm, and fertilization and hatching rates of fresh sperm and sperm stored for 0, 30 and 60 min at 4°C post‐thawing. After being frozen in liquid nitrogen and then thawed, the percentage of motile sperm and duration of motility were not affected by 30 min of storage at 4°C, whereas a significant decline in these parameters was observed after 60 min of storage. Similarly, fertilization and hatching rates were significantly affected within 60 min of storage at 4°C, and the fertility of frozen‐thawed sperm was significantly lower than that of fresh sperm. We conclude that cryopreserved sperm of beluga sturgeon could be stored for 30 min without the loss of sperm quality. This described procedure for beluga sturgeon cryopreservation is reliable and efficient and therefore can be recommended for hatchery practice after scaling up this technique.  相似文献   

The Influence of Morphology,Follicle Size and Bcl‐2 and Bax Transcripts on the Developmental Competence of Bovine Oocytes     

THC De Bem  PR Adona  FF Bressan  LG Mesquita  MR Chiaratti  FV Meirelles  CLV Leal 《Reproduction in domestic animals》2014,49(4):576-583

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Comparison of Free Air Space Test Methods     

Danlin Su  Daryl McCartney  Qunfeng Wang 《Compost science & utilization》2013,21(2):103-113

Air space within a compost material can be measured using several methods. This project, focusing on bulking materials, compared five air space (AS) test methods: particle density (PD) method, modified PD (MPD) method, air pycnometer (AP) method, modified air pycnometer (MAP), and a quick method. There were significant differences between results obtained using the different tests. PD and MAP methods yielded results representing total air space (TAS), while the AP and MPD methods yielded values which were thought to represent free air space (FAS). The Quick method resulted in AS values dramatically different from the other methods, and further development and validation of the method was recommended. The MPD was found to be unsuitable for samples containing fine material, such as biosolids. Both the PD and the MAP tests were precise; however, results were biased to TAS values. This was illustrated through the similarity between AS values measured by the two tests and the predicted TAS. The MAP was the simplest method and was recommended for use with compost samples. Strong linear correlations were observed between TAS and bulk density (BD; kg m^?3: TAS = 100 – 0.096*BD (R²=0.98) for PD and 100 – 0.090*BD (R²=0.90) for MAP. The effect of storage time on FAS, TAS, and moisture content was examined in this study. Straw samples were stored for a month and showed no change in the physical properties. Woodchip samples were stored for over 5 months. A slight change in both FAS and TAS was observed, but the differences were minor compared to variations induced by different particle sizes and moisture levels.  相似文献   

Vaccination of pigeons against paramyxovirus 1 infection     

P G Box  H C Holmes  E McCartney 《The Veterinary record》1985,117(21):555-556

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Rhodococcus equi-associated osteomyelitis in foals     

EC FIRTH  MR ALLEY†  H HODGE 《Australian veterinary journal》1993,70(8):304-307

Two cases of Rhodococcus equl infection in foals are described, in which osteomyelitis was a feature. Because rhodococcal infection is usually low grade and chronic, and because the signs of early metaphysitis can be subtle, any articular or periarticular swelling in a foal from a farm with a history of rhodococcosis should be strongly suspected to be associated with R equl until proven otherwise.  相似文献   

Reply     

MR ALDERTON 《Australian veterinary journal》1993,70(11):429-430

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Use of an acrylic external fixator with an intramedullary tie-in pin for the treatment of tibial fractures in 85 dogs     

McCartney WT 《The Veterinary record》2007,161(17):596-597

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Relative contributions of sedimentation and impaction to deposition of particles in a crop canopy     

H.A. McCartney  D.E. Aylor 《Agricultural and Forest Meteorology》1987,40(4)

Lycopodium spores were released steadily into the air during 20–30 min from a line source positioned within a wheat crop. The spores were trapped on sticky strips held at angles, π, of 0, 30, 60 and 90° with respect to the horizontal and oriented to face the mean wind direction and on sticky, vertical glass rods. The aerial spore concentration, C, was measured by small suction traps. Deposits of the spores on wheat leaves were obtained from sections of leaves whose posture in the canopy was nearly horizontal, nearly vertical, or at angles between 30 and 60°. Number of spores per m² for all trapping surfaces were obtained by counting under a microscope. Experiments were conducted on seven different days, encompassing friction velocities, u^*, of 0.27–0.50 m s⁻¹. The rate of deposition on angled surfaces, D(π), was given approximately by D(θ) = D(0) cos (θ) + D(90) sin (θ), where D(0) and D(90) were the observed rate of deposit on horizontal and on vertical surfaces, respectively. Below mid-canopy height, inertial impaction of spores was negligible, so that D(90) = 0 for all the trap surfaces. There, D(0) was mainly due to sedimentation and was very nearly equal to v_s·C, where v_s is the settling speed of the spore in still air. Near the top of the canopy, deposition on sticky surfaces was enhanced by inertial impaction and turbulent deposition, so that D(0) was about twice that expected from sedimentation and D(90) was about five times larger than expected from inertial impaction at the mean wind speed. Nevertheless, considering the vertical distribution of leaf area and the angles of leaves in a wheat canopy, the rate of deposition of spores for the entire depth of a wheat canopy can be calculated with a probable underestimation of only 20% by simply assuming sedimentation on horizontally projected area and impaction on vertically projected area.  相似文献