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Recent advances in sequencing technology have brought several novel platforms for marker development and subsequent genotyping. The high-throughput and cost effective marker techniques have changed the entire scenario of marker applications. The huge genotypic data obtained with next generation sequencing (NGS) also demands analytical tools, statistical advances, and comprehensive understanding to cope with breeding applications. In the present review, we discussed different available marker techniques, their strengths, and limitations. Emphasis was given on software tools, analytical pipelines, workbenches, and online resources available for marker development. Comparison of SNP genotyping involving complexity reduction techniques like GBS, RRL, RAD, and array-based platforms were presented in a view to describe suitability for specific purposes. We found that genotyping by whole genome re-sequencing has great potential, and could be a routine application in the near future with continuously decreasing cost of sequencing. Microsatellites, still a valuable option for breeders, have also advanced with NGS. Here a catalogue of tools for microsatellite evaluation in short sequence reads was provided. The most common applications of molecular marker like QTL mapping, genome-wide association mapping (GWAS), and genomic selection were highlighted. The present review will be helpful for the effective utilization of available resources and for the planning of crop improvement programs employing molecular marker techniques.  相似文献   
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Summary

Histological examination of 753 Beagle dogs showed renal foci of calcification in 27.9% of males and 21.5% of females. In contrast, stomach calcification was observed in 6.2% of males and 5.2% of females examined.

It is suggested that the focal calcification seen in both organs was possibly of dietary origin and may have been related to the phosphate content.  相似文献   
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Cuttage propagation of Aesculus indica was tested by treatments with different concentrations of indole-3-acetic acid (IAA) @ 2000, 4000 (ppm), indolebutyric acid (IBA) @ 2000, 4000 (ppm) and naphthlcetic acid (NAA) @ 2000, 4000 (ppm) in dry formulation in the Forest Nursery, Faculty of Forestry, SKUAST-K, Shalimar. The cuttings treated with IBA @ 4000 ppm and IBA @ 2000 ppm had a sprouting rate of 75% and 50%, respectively, which was significantly higher than that of control and other treatments. The highest rooting rate (50%) was recorded in the cuttings with the application of IBA @ 4000 ppm. The cuttings treated with IBA @ 2000 ppm had 25% rooting rate. All other treatments along with control (talc powder) failed to induce rooting. It was concluded that IBA @ 4000 ppm was a better-applied concentration for vegetative propagation of A. indica under Kashmir conditions. Biography: Mumtaz Majeed (1980– ), male, post graduate in Faculty of Forestry, Sher-e-Kashmir University of Agricultural Science & Technology, Shalimar, Srinagar - 191 121, J&K, India.  相似文献   
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Vermicomposts are produced through interactions between earthworms and microorganisms in the breakdown of organic wastes. Aqueous extracts were prepared in commercial brewing equipment (Growing Solutions Inc.) from vermicomposts processed from pre-consumer food waste. The ratio of vermicompost to water was 1 to 5 v:v to produce a 20% aqueous solution. The effects of soil drenches at dilutions of 20%, 10%, and 5% vermicompost extracts, were compared with those of deionized water, in the suppression of green peach aphids, mealybugs, and two spotted spider mites attacking tomatoes and cucumbers, in greenhouse cage experiments.  相似文献   
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Tropical Animal Health and Production - Assessment of the ruminant livestock feed sources availability, existing feeding practices, and nutrient balances was embarked at different zones of Gurez...  相似文献   
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3S技术可快速、准确、实时地获取、处理空间信息,具有极大的应用价值。该文介绍了3S技术在研究野生动物生境格局、生境破碎化、生境因子特性、生境分析模型建立、生境评价等诸多方面中的应用,综合了近年来的最新研究进展,并探讨了今后3S技术在野生动物生境研究中的应用前景。  相似文献   
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