Recovery of brodifacoum in vomitus following induction of emesis in dogs that had ingested rodenticide bait     

KH Parton  EK Willson  MG Collett  LH Booth 《New Zealand veterinary journal》2018,66(1):41-43

AIM: To assess the benefit of inducing emesis in dogs that have ingested rodenticide bait containing brodifacoum (BDF), by determining the amount of BDF in bait recovered from the vomitus relative to the estimated amount consumed.

METHODS: Between 2014 and 2015 samples of vomitus from seven dogs that ingested rodenticide baits containing BDF were submitted by veterinarians in New Zealand. All seven dogs had been given apomorphine by the veterinarian and vomited within 1 hour of ingesting the bait. Some or all of the bait particles were retrieved from each sample and were analysed for concentrations of BDF using HPLC. Based on estimations of the mass of bait consumed, the concentration of BDF stated on the product label, and the estimated mass of bait in the vomitus of each dog, the amount of BDF in the vomited bait was calculated as a percentage of the amount ingested.

RESULTS: For five dogs an estimation of the mass of bait ingested was provided by the submitting veterinarian. For these dogs the estimated percentage of BDF in the bait retrieved from the vomitus was between 10–77%. All dogs were well after discharge but only one dog returned for further testing. This dog had a normal prothrombin time 3 days after ingestion.

CONCLUSIONS AND CLINICAL RELEVANCE: The induction of emesis within 1 hour of ingestion can be a useful tool in reducing the exposure of dogs to a toxic dose of BDF. The BDF was not fully absorbed within 1 hour of ingestion suggesting that the early induction of emesis can remove bait containing BDF before it can be fully absorbed.  相似文献   

Veränderungen der Vegetation durch experimentelle Erdgasbehandlung     

PETR PY&#;EK  ANTONÍN PY&#;EK 《Weed Research》1989,29(3):193-204

Changes in vegetation caused by experimental leakage of natural gas The response of vegetation to natural gas leakage was studied in Central Bohemia, Czechoslovakia, in 1978–84. The gas used was of the following chemical composition: 93·8% methane, 3·6% ethane, 0·8% propane, 0·25% butane, 0·3% carbon dioxide. The test crops were planted in 60·5 m plots containing underground bores from which gas was released into the soil through distribution pipes. In addition, the response of some weed species was recorded. The important effects of contamination observed were restricted growth and decrease in the number of individuals present. A change in the green colour of leaves was recorded in Medicago sativa, Brassica oleracea var. acephala, Secale cereale, Lolium multiflorum. Beta vulgaris and Zea mays. An obvious shift of developmental stages was found in Solanum tuberosum. Helianthus annuus and Zea mays (delay of recruitment and blooming). Restricted or even failure of reproduction can be considered the typical effect of exposure to natural gas: this holds true especially for Allium cepa, Triticum aestivum, Hordeum distichon, Trifolium sp.div., Medicago sativa, and most of the weedy species. Various growth deformations, mostly found in the underground parts of the plants (tubers and roots in Solanum tuberosum, Beta vulgaris), can be ascribed to the unfavourable conditions of soil aeration. Plants of Chenopodium album and Medicago sativa growing in the vicinity of the gas release point had an increased number of stomata. There were changes in the course of the reflectance curves estimated in the population of Trifolium pratense subsp. sativum. There was a conspicuous decrease of the near infrared reflectance in the stand exposed to gas in comparison with the control. Decrease of coverage and density of the stands reduced species diversity and changes in the species' composition (reduction of sensitive species) are the general consequences of natural gas leakage. The first symptoms on the vegetation were observed after 15–30 days which corresponds to about 80–150 m³ of discharged gas.  相似文献   

Kinetics of leaf temperature fluctuation affect isoprene emission from red oak (Quercus rubra) leaves     

Singsaas EL  Laporte MM  Shi JZ  Monson RK  Bowling DR  Johnson K  Lerdau M  Jasentuliytana A  Sharkey TD 《Tree physiology》1999,19(14):917-924

Because the rate of isoprene (2-methyl-1,3-butadiene) emission from plants is highly temperature-dependent, we investigated natural fluctuations in leaf temperature and effects of rapid temperature change on isoprene emission of red oak (Quercus rubra L.) leaves at the top of the canopy at Harvard Forest. Throughout the day, leaves often reached temperatures as much as 15 degrees C above air temperature. The highest temperatures were reached for only a few seconds at a time. We compared isoprene emission rates measured when leaf temperature was changed rapidly with those measured when temperature was changed slowly. In all cases, isoprene emission rate increased with increasing leaf temperature up to about 32 degrees C and then decreased with higher temperatures. The temperature at which isoprene emission rates began to decrease depended on how quickly measurements were made. Isoprene emission rates peaked at 32.5 degrees C when measured hourly, whereas rates peaked at 39 degrees C when measurements were made every four minutes. This behavior reflected the rapid increase in isoprene emission rate that occurred immediately after an increase in leaf temperature, and the subsequent decrease in isoprene emission rate when leaf temperature was held steady for longer than 20 minutes. We concluded that the observed temperature response of isoprene emission rate is a function of measurement protocol. Omitting this parameter from isoprene emission models will not affect simulated isoprene emission rates at mild temperatures, but can increase isoprene emission rates at high temperatures.  相似文献   

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality          下载免费PDF全文

F Krania  E Dovolou  CA Rekkas  EK Theodosiadou  I Pappas  GS Amiridis 《Reproduction in domestic animals》2015,50(1):112-120

Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue‐type plasminogen activator (t‐PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus‐oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t‐PA and/or its inhibitor epsilon‐aminocaproic acid (control, t‐PA, t‐PA+ε‐ACA, ε‐ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t‐PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε‐ACA. PAA in the treated group was significantly reduced by ε‐ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t‐PA‐modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t‐PA. In conclusion, it appears that excessive t‐PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.  相似文献   

Enzyme-linked immunosorbent assay (ELISA) for the detection of spring viraemia of carp virus (SVCV) in tissue homogenates of the carp, Cyprinus carpio L.   总被引：2，自引：0，他引：2  

L. RODÁK  Z. POSPÍ&#;IL  J. TOMÁNEK  T. VESELÝ  T. OBR  L. VALÍ&#;EK 《Journal of fish diseases》1993,16(2):101-111

Abstract. An enzyme-linked immunosorbent assay (ELISA) for the demonstration of the virus of spring viraemia of carp (SVCV) in liver, kidney and spleen homogenates, and in infected cell cultures is described. The sensitivity of the method is 10^2·8–10^3·5 TCID₅₀ 0·lml⁻¹ of the examined fluid. The specificity has been confirmed by the ELISA inhibition test and by results of virological examinations. Contamination with bacteria or fungi of samples taken from dead fish had no effect on the results of ELISA. Specific anti-SVCV sera were used successfully for the production of conjugates for the direct immunoperoxidase and immunofluorescence detection of SVCV in infected cell cultures.  相似文献   

Feeding value of pastures for horses     

SO Hoskin  EK Gee 《New Zealand veterinary journal》2013,61(6):332-341

The feeding value of fresh pasture grazed in situ is determined by animal performance or productivity and could be relatively easily established for growing and lactating horses. Despite this, there is a lack of published information on the relative feeding value of different pastures and forages grazed by horses in New Zealand and the world. In addition, for adult breeding or non-breeding and young or adult sport or performance horses, the definition of feeding value and its determination remain problematic.

Limited information suggests that the feeding value of perennial ryegrass-based pasture in New Zealand for young growing horses is high, and growth rates for Thoroughbred horses fed solely on pasture in New Zealand are similar to those reported from the Northern Hemisphere where grain-based supplements are fed in addition to pasture or other forages. Attempts to assess the ability of fresh pastures to meet the nutrient requirements of horses are hampered by problems associated with determination of feed intake by grazing horses and lack of knowledge of the digestibility and utilisation of digested nutrients, including the relative bio availability of macro- and micro-minerals in pasture. A further challenge for future research is to determine the effect of herbage allowance and grazing behaviour, including pasture species preferences, on voluntary feed intake by grazing horses.

Grazing pasture has benefits for equine health and well-being including reduced risk of some nutrition-related disorders and reduced prevalence of stereotypic behaviour. Pastured horses have greater freedom for expression of natural behaviours including social interaction and exercise. However, grazing pasture is also associated with animal health problems, particularly parasitism and diseases related to pasture-associated toxins.  相似文献   

Variation in competitive abilities of plants and microbes for specific amino acids   总被引：21，自引：0，他引：21  

D. A. Lipson  T. K. Raab  S. K. Schmidt  R. K. Monson 《Biology and Fertility of Soils》1999,29(3):257-261

 Microbes are assumed to possess strong competitive advantages over plants for uptake of nutrients from the soil. The finding that non-mycorrhizal plants can obtain a significant fraction of their N requirement from soil amino acids contradicts this assumption. The amino acid glycine (Gly) has been used as a model amino acid in many recent studies. Our preliminary studies showed that Gly was a poor substrate for microbial growth compared to other amino acids. We tested the hypothesis that the alpine sedge Kobresia myosuroides competes better for Gly than for other amino acids because of decreased microbial demand for this compound. Soil microbial populations that could grow using Gly as a sole carbon source were about 5 times lower than those that could grow on glutamate (Glu). Gly supported a significantly lower population than any of the ten other amino acids tested except serine. In contrast, K. myosuroides took up Gly from hydroponic solution at faster rates than Glu. In plant-soil microcosms, plants competed with soil microbes 3.25 times better for Gly than for Glu. We conclude that the low microbial demand and the rapid plant uptake of Gly relative to other amino acids allow Gly to be an especially important nitrogen source for K. myosuroides. Received: 9 February 1998  相似文献   

The influence of diel period on fish assemblage in the unstructured littoral of reservoirs     

M. ŘÍHA  J. KUBEČKA  M. PRCHALOVÁ  T. MRKVIČKA  M. ČECH  V. DRAŠTÍK  J. FROUZOVÁ  E. HOHAUSOVÁ  T. JŮZA  M. KRATOCHVÍL  J. PETERKA  M. TUŠER  M. VAŠEK 《Fisheries Management and Ecology》2011,18(4):339-347

Abstract Diel changes in littoral fish assemblage were studied in four reservoirs in the Czech Republic (Central Europe). The sampling was performed by beach seining in an unstructured littoral zone. Perch, Perca fluviatilis L., roach, Rutilus rutilus (L.), bream, Abramis brama (L.), carp, Cyprinus carpio L., ruffe, Gymnocephalus cernua (L.), pikeperch, Sander lucioperca (L.), eel, Anguilla anguilla (L.) and A. brama × R. rutilus hybrids exhibited higher densities at night. Only bleak, Alburnus alburnus (L.) exhibited higher densities during the day. The number of species was higher in night hauls, and mean body size of roach and bleak was also higher at night. Diel changes in fish densities resulted in the change in species composition between day and night. The study has implication for the sampling design to assess littoral fish assemblages using beach seine netting and recommends night sampling for a representative assessment or sampling during both diel periods for a robust assessment.  相似文献   

Arctic environment and resources: by John E. Sater,A. G. Ronhovde & L. C. Van Allen. Arctic Institute of North America,Washington, D.CC.: 310 pp., illustr., 26·3 × 17 cm, $10, 1972     

Anna P.B. Monson 《Biological conservation》1973,5(3):241

  相似文献   

Integration of pines and pastures for hay and grazing     

C. E. Lewis  G. W. Burton  W. G. Monson  W. C. McCormick 《Agroforestry Systems》1984,2(1):31-41

When combining pine and cattle production on improved pastures, grazing may have to be delayed for several years until trees are large enough to resist injury. During this period forage would be lost unless harvested for hay. This study in south Georgia, USA, examined hay production during the first 3 years, cattle production during the next 3 years, and effects of this management system on survival and growth of slash pine(Pinus elliottii) planted in widely-spaced rows within the pastures. Pines were planted at 225 trees/ha in configurations of 3.0 x 14.6 or 4.9 x 9.1 m in pastures recently sprigged or seeded with Coastal bermudagrass (Cynodon dactylon) or Pensacola bahiagrass(Paspalum notatum). Pastures were fertilized annually and cut for hay four or five times each year. Wide-row spacings permitted haying operations, but tree rows and turning areas removed 5 to 8 percent of the area from production. However, hay yields and liveweight gains by yearling cattle were near normal for this locale. Slash pine planted in these fertilized pastures survived well, grew rapidly, and only a few trees were killed during haying operations. After 6 years, 83 percent of the trees survived and averaged 6.5 m in height and 13.2 cm in diameter. Cattle killed a few trees by rubbing during the 4th year. Wide-row spacing of pines permits harvesting forage for hay while waiting for the trees to become large enough to permit grazing. This approach to agroforestry produces annual returns to the landowner while awaiting maturity of the pines.  相似文献