Isolation and Identification of Enterococcus faecalis from Fur-bearing Animals     

CI Yang  SHI Xiaojie  SONG Peng  DING Qi  LI Wenli 《中国畜牧兽医》2007,47(9):2997-3005

In order to obtain Enterococcus faecalis from fur animals and evaluate its prebiotic properties,in this study,Enterococcus faecalis was isolated from the feces of healthy adult fur-bearing animals (mink,fox,raccoon dog),identified by morphological observation,biochemical test and 16S rRNA sequence analysis.The growth curve,acid production capacity and antibiotic sensitivity of the Enterococcus faecalis isolates were measured to evaluate their probiotic properties.Some strains were selected to determine their tolerance to temperature,artificial gastric juice and artificial bile salt.The results showed that five strains were Gram-positive,and their biochemical characteristics were basically consistent with the standard strains of Enterococcus faecalis,and they were identified as Enterococcus faecalis by 16S rRNA sequence analysis.The five strains all entered the logarithmic phase at 2 h after culture,and entered the stable phase at 8-10 h,and had weak acid production capacity.The resistance rate of the isolates to tetracycline and levofloxacin was 100%,followed by penicillin (80%),erythromycin (80%),gentamicin (80%) and chloramphenicol (40%).All the isolates were sensitive to ampicillin and vancomycin.Enterococcus faecalis from mink,fox and raccoon dog had strong tolerance to temperature below 60 ℃,artificial gastric juice with pH>3.0 and 0.3%-0.5% concentration of bile salt,but poor tolerance to temperature above 70 ℃,and artificial gastric juice with pH<3.0.In conclusion,five strains of Enterococcus faecalis from fur animals (mink,fox,raccoon dog) were obtained in this study.The isolated strains propagated rapidly,which were suitable for colonization and played a prebiotic role in fur animals' intestines,and had good prebiotic characteristics and stress resistance.They could be used as candidate strains for animal microbiological agents for further study.  相似文献   

西藏波密县天麻产业发展问题研究   总被引：1，自引：0，他引：1  

次仁卓嘎  沈娟 《现代农业科学》2009,(5):269-270

由于西藏波密县农业生产具有地质史独特,地形地貌复杂,气候异常,土壤种类繁多,野生植被多样的特点,给天麻生产带来机遇的同时,又带来了严峻的挑战。笔者主要从西藏波密县天麻生产的现状入手,科学地分析了西藏波密县天麻生产中存在的问题,提出了天麻生产发展的对策。  相似文献   

四翅滨藜在固阳县的引种试验研究     

段霞  鲁海  次国鑫 《内蒙古林业调查设计》2009,32(1):44-45

包头固阳地区自然气候条件恶劣,可供造林的树种贫乏,四翅滨藜耐干旱、瘠薄、耐盐碱,适应性强,既是林又是草,是林草兼用的饲料植物,对牲畜有较好的适口性。四翅滨藜可采用播种子和扦插繁育,但用半木质化的嫩枝扦插繁殖成苗率高,生产成本低,技术简单。是包头固阳地区造林、盐碱地改造、荒山营建灌木林的较理想的树种之一。  相似文献   

Effects of exercise conditioning on semen characteristics from hyper- and hypotensive lines of turkeys     

L M Krista  E E Pierson  G R McDaniel  E C Mora  J A McGuire  S L Bolden  L E Miller 《British poultry science》1985,26(3):349-356

Twenty-one male Broad Breasted White turkeys which had been selected for high or low blood pressure were put on an exercise regimen to determine its effect on semen quality. Eleven were from the hypertensive line and 10 from the hypotensive line. Eleven (6 hypertensive and 5 hypotensive) turkeys were placed on an exercise regime of walking (herding) about 1 mile daily for 6 weeks before the experiment began, and then for a further 6 weeks during semen collection. Semen was collected from 11 turkeys on the exercise regime and 10 nonexercised control birds which were kept in their experimental pens during the trial. Both hypertensive and exercised groups showed an improved semen colour score at the first collection period (P less than or equal to 0.09). Only the exercised group showed a significantly (P less than or equal to 0.05) better score than the control group during periods 2 and 3. The hypertensive line showed significantly higher spermatozoa count than the hypotensive line during the first collection period. There was no effect of either exercise or selection for high or low blood pressure on semen volume.  相似文献   

ULMUS ANTI- INSECT FAST- GROWING NEW VARIETY LAYER CUTTING REPRODUCTION TECHNOLOGY     

Du ran  LAN Zai-ping  MA Ke  CI Zhong-ling 《内蒙古农业大学学报(自然科学版)》2008,29(4)

  相似文献   

土壤质量生物学指标研究进展   总被引：59，自引：7，他引：52  

唐玉姝  魏朝富  颜廷梅  杨林章  慈恩 《土壤》2007,39(2):157-163

本文对近年土壤微生物、土壤酶活性和土壤动物等土壤质量生物学指标研究成果进行了综合评述。土壤微生物是土壤有机组分和生态系统中最活跃的部分,被认为是最敏感的土壤质量生物学指标:微生物生物量代表参与调控土壤中能量和养分循环及有机物质转化所对应微生物的数量,但须结合多样性研究以弥补其无法反映土壤微生物组成和区系变化的缺陷;微生物群落组成和多样性动态反映土壤中生物类群的多变性和土壤质量在微生物数量和功能上的差异;土壤微生物活性体现在土壤微生物商、微生物呼吸和代谢商等方面,应考虑生物量大小与微生物种群活性间的相关关系以反映微生物种群内的差异。土壤酶活性具有极高时效性,在较短时间内就能反映出土壤质量的变化。土壤动物通常以种类的组成和数量,土壤动物区系的相对丰度、多样性或活性作为评价土壤生物质量的敏感指标。与土壤理化指标相比,土壤生物学指标更能对土壤质量的变化做出灵敏迅速的响应,因而被广泛地用于评价土壤质量。  相似文献   

化肥减量配施对燕山丘陵区春玉米生长发育及产量的影响     

边丽梅  董喆  郑伟  张丽妍  张昊  郝春雷  慈艳华  杜江洪  孟繁盛 《陕西农业科学》2021,(4):7-11

针对燕山丘陵区长期过量施用化肥导致肥料利用率低的现状,研究了有机肥、缓控释尿素、秸秆还田和生物炭与化肥减量配施对玉米生长发育和产量的影响,探讨了化肥减量替代的可行性,旨在探索燕山丘陵区春玉米田化肥减量配施技术模式。试验采用随机区组设计的方法,以赤单218为试验材料,设置平衡施用化肥（NPK）、NPK基础上减氮20%（RN）、RN基础上有机肥替代总氮量的20%（RN+OM）、RN基础上缓控释尿素替代总氮量的40%（RN+CRU）、RN基础上秸秆全量还田（秸秆还田）和RN基础上生物炭基肥替代总氮量的20%（生物炭肥）共6个处理,研究燕山丘陵区化肥减量配施对春玉米生长发育及产量的影响。结果表明,减氮配施可以增加玉米产量、玉米叶面积指数和地上部干物质量,对穗粗、穗长、行粒数和千粒重也有积极的影响。减氮配施有机肥条件下,玉米产量最高,相应的产量构成因素穗粗、穗长、行粒数和千粒重也最高,。其次是减氮配施缓控释尿素处理,而秸秆还田和生物炭肥配施处理的表现却并不突出,综合考虑作物产量与环境保护等因素,在春玉米生产中减少氮肥用量、施用有机肥和缓控释尿素可以保证产量,同时还能减少农业面源污染,值得在生产中大力推广。  相似文献   

A Large‐scale,Rapid Public Health Response to Rabies in an Organ Recipient and the Previously Undiagnosed Organ Donor     

R. M. Wallace  D. Stanek  S. Griese  D. Krulak  N. M. Vora  L. Pacha  V. Kan  M. Said  C. Williams  T. H. Burgess  S. S. Clausen  C. Austin  J. Gabel  M. Lehman  L. N. Finelli  G. Selvaggi  P. Joyce  F. Gordin  D. Benator  A. Bettano  S. Cersovsky  C. Blackmore  S. V. Jones  B. D. Buchanan  A. I. Fernandez  D. Dinelli  K. Agnes  A. Clark  J. Gill  M. Irmler  D. Blythe  K. Mitchell  T. J. Whitman  M. J. Zapor  S. Zorich  C. Witkop  P. Jenkins  P. Mora  D. Droller  S. Turner  L. Dunn  P. Williams  C. Richards  G. Ewing  K. Chapman  C. Corbitt  T. Girimont  R. Franka  S. Recuenco  J. D. Blanton  K. A. Feldman 《Zoonoses and public health》2014,61(8):560-570

This article describes and contrasts the public health response to two human rabies cases: one organ recipient diagnosed within days of symptom onset and the transplant donor who was diagnosed 18 months post‐symptom onset. In response to an organ‐transplant‐related rabies case diagnosed in 2013, organ donor and recipient investigations were conducted by multiple public health agencies. Persons with potential exposure to infectious patient materials were assessed for rabies virus exposure. An exposure investigation was conducted to determine the source of the organ donor's infection. Over 100 persons from more than 20 agencies spent over 2700 h conducting contact investigations in healthcare, military and community settings. The 564 persons assessed include 417 healthcare workers [5.8% recommended for post‐exposure prophylaxis (PEP)], 96 community contacts (15.6% recommended for PEP), 30 autopsy personnel (50% recommended for PEP), and 21 other persons (4.8% recommended for PEP). Donor contacts represented 188 assessed with 20.2% recommended for PEP, compared with 5.6% of 306 recipient contacts recommended for PEP. Human rabies cases result in substantial use of public health and medical resources, especially when diagnosis is delayed. Although rare, clinicians should consider rabies in cases of encephalitis of unexplained aetiology, particularly for cases that may result in organ donation.  相似文献   

“稻转旱”对低山丘陵区氮素流失通量的影响——以三峡库区渠溪小流域为例     

冉娇娇  黄平  陈成龙  慈恩  吕明权 《水土保持研究》2022,29(2):70-75

为探究“稻转旱”的土地利用变化对区域氮素流失的影响,以三峡库区渠溪小流域为研究对象,利用土地利用转移矩阵揭示了该流域2001—2019年的土地利用变化,基于输出系数模型并利用流域氮负荷实测数据进行模型参数修订后,对该流域2001年和2019年氮负荷进行了估算并比较。结果表明:该流域2001—2019年土地利用总体演变趋势为水稻田向旱地转化、旱地向林地转化,且以“稻转旱”为主。这种演变使流域氮素流失增加,2019年相对于2001年氮流失量增加了26%,平均每公顷氮流失量增加了约7.8 kg。这种变化表明在农业结构调整时应考虑到水稻田对面源污染物氮素良好的净化拦截作用,控制其面积的缩减以及在小流域氮素输移关键节点上的布局。  相似文献   

Lancaster类群玉米自交系穗长的遗传研究     

郑雷雷  胡洋  孙贵星  杨巍  慈佳宾  杨伟光 《吉林农业大学学报》2014,(5)

利用完全和不完全双列杂交材料对玉米穗长进行了杂种优势分析和遗传模型测验。结果表明:以Lancaster类群为母本的杂交组合F1代中合344优势均值最高(53.26%),一般配合力适中(0.914 1),生产上加以利用可组配出超亲中穗品种;Mo17由于本身为长穗自交系,所以优势均值适中(29.04%),但一般配合力最高(7.585 2)。通过对Lancaster类群玉米穗长的遗传模型测验可知,玉米穗长的遗传符合加性-显性模型,加性效应和显性效应真实存在,且加性效应更为重要。Mo17穗长的一般配合力最高,是最有潜力组配出中-长穗品种的亲本。  相似文献