排序方式： 共有33条查询结果，搜索用时 31 毫秒
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Veronica Redaelli Domenico Bergero Enrica Zucca Francesco Ferrucci Leonardo Nanni Costa Lorenzo Crosta Fabio Luzi 《Journal of Equine Veterinary Science》2014
This review of the use of thermographic technique in equines introduces the principles upon which infrared radiation and thermoregulatory physiology are based and describes the instrumentation used and its practical use. The advantage of this imaging technique is that it is a noninvasive thermographic examination, both from an operational (the animal and the operator) and health (no penetrating radiation is used) standpoint. Advantages and disadvantages of this technique, equine applications, and physiological assessments are discussed. 相似文献
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M F Borgerding R D Hicks J E Bodnar D M Riggs E J Nanni G W Fulp W C Hamlin J A Giles 《Journal of the Association of Official Analytical Chemists》1990,73(4):605-609
The design of a new cigarette that heats rather than burns tobacco calls for modifications to the Federal Trade Commission (FTC) method for analytical smoking. These changes include eliminating sample conditioning at 75 degrees F and 60% RH, exercising greater care in lighting cigarettes, and smoking cigarettes to self-extinguishment rather than to a predetermined butt length as a measure of complete consumption. By several gross analytical measures, smoke condensate from the new cigarette differs substantially from that of tobacco-burning cigarettes. This is inferred from the lack of coloration of smoke condensate collected on Cambridge filters. Elemental analysis demonstrates reduced carbon and nitrogen content concurrent with increased hydrogen. Thermogravimetric analysis shows almost quantitative weight loss at Tmax = 220 degrees C. Ultraviolet (UV) spectrophotometric analysis shows greatly reduced levels of tobacco-derived smoke components and qualitative differences in chemical entities being measured. By design, the heat required for smoke formation is supplied by a carbon heat source embedded in the cigarette tip. Tobacco contained in the cigarette is not burned and is exposed to temperature less than 300 degrees C. Thus, it is apparent (1) that smoke from the new cigarette contains little or no "tar" as tar is classically defined, and (2) that the FTC method even as modified to account for cigarette design differences is appropriate only for determination of nicotine and carbon monoxide yielded from this cigarette. 相似文献
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Bovine herpesvirus‐1 (BHV‐1) and bovine herpesvirus‐5 (BHV‐5) are closely related viruses which exhibit some important differences at the genetic and immunogenic levels which may explain the differences in their pathogenicity and epidemiological characteristics. A multiplex polymerase chain reaction (M‐PCR) was developed to detect and differentiate between BHV‐1 and BHV‐5. In this M‐PCR two pairs of primers (TK1, TK2 and GD1, GD2) were used in the same reaction mix to amplify a thymidine kinase genomic region (183 bp) of BHV‐1 and one genomic region of the glycoprotein D (564 bp) of BHV‐5. The specificity of the M‐PCR was demonstrated when using both primers pairs simultaneously with BHV‐1 and BHV‐5 templates. The two expected bands were amplified without the apparition of non‐specific products. However, when other herpesvirus strains were used, there was no amplification. To evaluate the sensitivity of the assay, dilutions of purified viral DNA were made for M‐PCR amplification. The detection limit was 7 pg for BHV‐1 and 22 pg for BHV‐5. It was also determined by comparing the M‐PCR with viral isolation. M‐PCR was able to detect one log10 more than viral isolation for BHV‐1 and for BHV‐5 was two logarithms lower. The applicability of M‐PCR was demonstrated on different specimens. Twenty isolates from field samples (11 BHV‐1 and nine BHV‐5) were positive by M‐PCR, and the results were completely coincident with previous characterization using the immunoperoxidase assay. M‐PCR could detect viral DNA in organ samples from natural infections, such as semen and brain. In addition, M‐PCR detected more positive samples than observation of the citophatic effect in cell culture of nasal swabs from experimentally infected animals in two different assays. Owing to the difference in size of the M‐PCR products which allows easy identification in an electrophoretic run, it is not necessary to use extra blotting and hybridization steps or a second round of amplification to differentiate clearly between BHV‐1 and BHV‐5. 相似文献
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Jacobsen S Niewold TA Halling-Thomsen M Nanni S Olsen E Lindegaard C Andersen PH 《Veterinary immunology and immunopathology》2006,110(3-4):325-330
The aim of the study was to determine the intraarticular serum amyloid A (SAA) response pattern in horses with inflammatory arthritis. Inflammatory arthritis was induced by injection of lipopolysaccharide (LPS) into the radiocarpal joint of four horses. Serum and synovial fluid (SF) samples were collected before and at 4, 8, 12, 24, 48, 72, 96, and 144 h after injection. Concentrations of SAA were measured by immunoturbidometry, and expression of SAA isoforms was visualized by denaturing isoelectric focusing and Western blotting. The LPS injection caused systemic and local clinical signs of inflammation. Serum amyloid A appeared in serum and SF within 8 h after LPS injection. Isoelectric focusing showed three major SAA bands with apparent isoelectric points (pI) of 7.9, 8.6, and >9.3 in serum and SF. Synovial fluid contained two additional isoforms with highly alkaline apparent pI values (apparent pI value extrapolated from standard curve = 10.0 and 10.2), which were not present in any of the serum samples. In conclusion, intraarticular injection of LPS induced systemic and local inflammatory responses in the horses. By demonstrating SF-specific SAA isoforms the results of the present study suggest that SAA is synthesized locally in the equine inflamed joint, similar to what has been demonstrated in humans previously. The marked local SAA synthesis suggests an important pathophysiological role in inflammatory arthritis. 相似文献
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OBJECTIVE: To determine serum amyloid A (SAA) concentrations in serum and synovial fluid from healthy horses and horses with joint disease and assess the effect of repeated arthrocentesis on SAA concentrations in synovial fluid. Animals-10 healthy horses and 21 horses with various types of joint disease. PROCEDURES: Serum and synovial fluid samples were obtained from each horse. In 5 of the 10 healthy horses, arthrocentesis was repeated 9 times. Concentrations of SAA were determined via immunoturbidometry. RESULTS: Serum and synovial fluid SAA concentrations were less than the assay detection limit in healthy horses and did not change in response to repeated arthrocentesis. Synovial fluid SAA concentrations were significantly higher in horses with suspected bacterial joint contamination or infectious arthritis, or tenovaginitis than in healthy controls, and serum concentrations were significantly higher in horses with infectious conditions than in the other groups. Neither serum nor synovial fluid SAA concentrations in horses with low-inflammation joint conditions differed significantly from those in healthy controls. Concentrations of SAA and total protein in synovial fluid were significantly correlated. CONCLUSIONS AND CLINICAL RELEVANCE: Synovial fluid SAA concentration was a good marker of infectious arthritis and tenovaginitis and appeared to reflect changes in inflammatory activity. The advantages of use of SAA as a marker include the ease and speed of measurement and the fact that concentrations in synovial fluid were not influenced by repeated arthrocentesis in healthy horses. Further study of the SAA response in osteoarthritic joints to assess its usefulness in diagnosis and monitoring of osteoarthritis is warranted. 相似文献
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添加物对小鼠腔前卵泡体外培养的影响 总被引：1，自引：0，他引：1
为探讨培养添加物对小鼠腔前卵泡生长成熟的影响,在培养液中分别加入不同体积分数的犊牛血清（ＦＣＳ）,牛卵泡液或添加促性腺激素,对小鼠腔前卵泡进行了培养。结果表明,５％以上犊牛血清明显提高腔前卵泡存活率,而１０％以上犊牛血清能明显促进卵泡的生长发育,２０％以上牛卵泡液可抑制卵母细胞的体外自发成熟分裂,并能促进腔前卵泡的生长发育,在不含犊牛血清的培养液中加入促卵泡素（ＦＳＨ）,促黄体素（ＬＨ）或ＦＳＨ＋ 相似文献
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Marcos Rafael Nanni Everson Cezar Guilherme Fernando Capristo Silva Anderson Antonio da Silva Gualberto 《Archives of Agronomy and Soil Science》2018,64(5):682-695
New techniques and improvements are required to quantify soil’s chemical and physical properties on production environment, reducing environmental impacts and minimizing soil analysis time. The aim of this study is to evaluate the possibility to estimate the content of silt, sand, clay, total iron and organic matter in soils formed by different lithologies in Parana State, Brazil, using VIS-NIR spectrum associated with Partial Least Square Regression (PLSR). 200 soil samples were collected in an area formed by Lixisols, Cambisols, Ferralsols, Arenosols and Nitisols in a depths of 0–0.2 and 0.2–0.8 m. Spectral readings were obtained in laboratory by FieldSpec 3 JR sensor. The spectral curves of the samples were correlated to the attributes through PLSR. The results obtained for sand in prediction were better when compared to the other attributes, presenting R2 = 0.90, r = 0.95 and RPD = 2.3. Clay and total iron presented satisfactory results, mainly for RPD values, which were above 2.4. Based on the results, it can be concluded that the PLSR technique associated with the spectral response of the soils, was able to estimate sand, clay and total iron with accuracy in a region formed by reworked materials, derived from several lithologies. 相似文献