Genetic Diversity and Allelic Frequency of Selected Thai and Exotic Rice Germplasm Using SSR Markers     

Wanwarang PATHAICHINDACHOTE  Natjaree PANYAWUT  Kannika SIKAEWTUNG  Sujin PATARAPUWADOL  Amorntip MUANGPROM 《水稻科学》2019,26(6):393-403

A collection of 167 Thai and exotic rice accessions was subjected for evaluation of genetic diversity and assessment of relationship by simple sequence repeat(SSR) markers. Among a total of 49 SSR markers, 13 markers distributing over 12 rice chromosomes showed clear polymorphic band patterns, and they were selected for genetic assessment. A total of 110 alleles were detected with an average of 8.46 alleles per locus. The averages of gene diversity, heterozygosity and polymorphic information content were 0.59, 0.02 and 0.56, respectively. The unweighted-pair group method with arithmetic averages(UPGMA) clustering analysis was performed for genetic distance, and phylogenetic tree was constructed. The result showed that this rice collection was divided into two major groups, classified as japonica and indica subspecies. Within the japonica group, temperate japonica and tropical japonica subgroups can be clearly separated. Three-dimensional principal component analysis projection and model-based population structure analysis showed consistent clustering results with two major groups of UPGMA analysis, supporting the classification of japonica and indica subspecies. The indica allelic frequency was also investigated to provide an indicative guide for breeders to overcome the practical problems on sterility of inter-subspecies hybrid offspring. This rice collection and information obtained in this study will be useful for rice breeding programs.  相似文献   

Development of new aromatic rice lines with high eating and cooking qualities          下载免费PDF全文

Kanokwan KAEWMUNGKUN  Keasinee TONGMARK  Sriprapai CHAKHONKAEN  Numphet SANGARWUT  Thiwawan WASINANON  Natjaree PANYAWUT  Khanittha DITTHAB  Kannika SIKAEWTUNG  QI Yong-bin  Sukanya DAPHA  Atikorn PANYA  Natthaporn PHONSATTA  Amorntip MUANGPROM 《农业科学学报》2023,22(3):679-690

Rice is the staple food for about half of the world’s population.Preferred by consumers,aromatic rice is a special type of rice with great commercial value.Cooking and eating qualities and aroma are the major grain qualities favored by most consumers.Currently,most of the available aromatic varieties have low yields and some undesirable agronomic traits.Thus,there is an urgent need to develop better aromatic rice varieties.This work aims to identify rice germplasm lines that have good grain qual...  相似文献   

Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice   总被引：1，自引：0，他引：1  

Sudthana KHLAIMONGKHON  Sriprapai CHAKHONKAEN  Keasinee PITNGAM  Khanittha DITTHAB  Numphet SANGARWUT  Natjaree PANYAWUT  Thiwawan WASINANON  Chareerat MONGKOLSIRIWATANA  Julapark CHUNWONGSE  Amorntip MUANGPROM 《水稻科学》2019,26(3):147-156

The discovery of thermo-sensitive genic male sterility(TGMS) has led to development of a simple and highly efficient two-line breeding system. In this study, genetic analysis was conducted using three F_2 populations derived from crosses between IR68301 S, an indica TGMS rice line, and IR14632(tropical japonica), Supanburi 91062(indica) and IR67966-188-2-2-1(tropical japonica), respectively.Approximately 1:3 ratio between sterile and normal pollen of F_2 plants from the three populations revealed that TGMS is controlled by a single recessive gene. Bulked segregant analysis using simple sequence repeat(SSR) and insertion-deletion(InDel) markers were used to identify markers linked to the tms gene. The linkage analysis based on the three populations indicated that the tms locus was located on chromosome 2 covering the same area. Using IR68301S × IR14632 F_2 population, the results showed that the tms locus was located between SSR marker RM12676 and InDel marker 2gAP0050058. The genetic distance from the tms gene to these two flanking markers were 1.10 and 0.82 cM, respectively.InDel marker 2gAP004045 located between these two markers showed complete co-segregation with the TGMS phenotype. In addition, InDel marker vf0206114052 showed 2.94 cM linked to the tms gene using F_2 populations of IR68301S × Supanburi 91062. These markers are useful tool for developing new TGMS lines by marker-assisted selection. There were ten genes located between the two flanking markers RM12676 and 2gAP0050058. Using quantitative real-time PCR for expression analysis, 7 of the 10 genes showed expression in panicles, and response to temperatures. These genes could be the candidate gene controlling TGMS in IR68301S.  相似文献