An ethnobotanical study among Albanians and Aromanians living in the Rraicë and Mokra areas of Eastern Albania     

Andrea Pieroni  Alban Ibraliu  Arshad Mehmood Abbasi  Vilma Papajani-Toska 《Genetic Resources and Crop Evolution》2015,62(4):477-500

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Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan)     

S. Ali  M. Ijaz  S. H. Farooqi  A. Z. Durrani  M. I. Rashid  A. Ghaffar  A. Ali  A. Rehman  S. Aslam  I. Khan  A. Masud  K. Mehmood 《Equine Veterinary Education》2021,33(2):75-83

Theileria equi (T. equi) is an obligate intra- and extra-erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross-sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero-biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2 , MG551915.1 and KY952237.1 , respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato-biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato-biochemical variations in horses.  相似文献   

Coumarins from the aerial part of Halocnemum strobilaceum     

Miftakhova AF  Burasheva GS  Abilov ZA  Ahmad VU  Zahid M 《Fitoterapia》2001,72(3):319-321

Four known coumarins, coumarin (1), 7-hydroxy-3-methylcoumarin (2), oreoselone (3) and heraclenin (4), were isolated from aerial part of Halocnemum strobilaceum. Their structures were determined by 1 and 2-D NMR techniques.  相似文献   

ULTRASONOGRAPHIC DETECTION OF FOREIGN BODIES IN SOFT TISSUES USING TURKEY MUSCLE AS A MODEL     

Zahid R.  Shah  MD  Jeffrey R.  Crass  MD  Dubravka C.  Oravec  MD  Errol M.  Bellon  MD 《Veterinary radiology & ultrasound》1992,33(2):94-100

Foreign bodies in the soft tissues are frequent problems in both veterinary and human medical practice. Radiography has been the principle tool for diagnosis, but is unable to define many foreign bodies. This study was undertaken to compare the sonographic and radiographic visualization for a variety of foreign bodies. Fresh turkey muscle (pectoral and thigh) with skin was used as the soft tissue model. Assorted foreign bodies were introduced 1 cm into the muscle. Materials examined were small wood fragments, steel wire, glass, nails, graphite, rock, BB pellets, and polystyrene plastic. Following introduction of the foreign bodies radiographs were obtained and the specimens were studied sonographically at 5.0 mHz and 7.5 mHz. All foreign bodies were visible with sonography. A bright echo with either acoustic shadowing or reverberations was present for all specimens. Radiographically metallic foreign bodies were visible but wood, some graphite, and plastic were not seen. We conclude that radiography should remain the primary imaging modality for foreign body detection because of its general availability and low cost. If radiography is negative ultrasound can detect and guide the removal of radiolucent foreign bodies.  相似文献   

Apo-9′-Fucoxanthinone,Isolated from Sargassum muticum,Inhibits CpG-Induced Inflammatory Response by Attenuating the Mitogen-Activated Protein Kinase Pathway     

Doobyeong Chae  Zahid Manzoor  Sung Chun Kim  Sohyun Kim  Tae-Heon Oh  Eun-Sook Yoo  Hee-Kyoung Kang  Jin-Won Hyun  Nam Ho Lee  Mi-Hee Ko  Young-Sang Koh 《Marine drugs》2013,11(9):3272-3287

Sargassum muticum (S. muticum) is a brown edible alga and widely distributed in Korea. This report was designed to evaluate the anti-inflammatory properties of apo-9′-fucoxanthinone (APO-9′) isolated from S. muticum on pro-inflammatory cytokine production. S. muticum extract (SME) exhibited significant inhibitory effects on pro-inflammatory cytokine production in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). APO-9′ pre-treatment in the CpG DNA-stimulated BMDMs and BMDCs showed a strong dose-dependent inhibitory effect on interleukin (IL)-12 p40, IL-6 and tumor necrosis factor (TNF)-α production with IC₅₀ values ranging from 5.31 to 13.79. It exhibited a strong inhibitory effect on the phosphorylation of ERK1/2 and on activator protein (AP)-1 reporter activity. APO-9′ pre-treatment exhibited significant inhibition of CpG DNA-induced production of inducible nitric oxide synthase. Taken together, these data suggest that SME and APO-9′ have a significant anti-inflammatory property and warrant further studies concerning the potentials of SME and APO-9′ for medicinal use.  相似文献   

Floristic composition and ecological gradient analyses of the Liakot Forests in the Kalam region of District Swat,Pakistan     

Sohail Anwar  Shujaul Mulk Khan  Zeeshan Ahmad  Zahid Ullah  Majid Iqbal 《林业研究》2019,(4):1407-1416

Kalam Kohistan is a dry,temperate hilly region in the Hindu Raj Series of the Greater Hindu Kush Mountains with diverse forests.As plant distribution and composition is expression of a range of various environmental variables,ecological and floristic attributes of vegetation in the Laikot Forests were therefore evaluated via consecutive explorative trips.These forests have not been evaluated ecologically before due to harsh climatic conditions and issues of accessibility and the study in hand is one of the first in its nature.Quantitative ecological techniques were used to sample forest vegetation and identify species in quadrats of 2 m×2 m for herbs,5 m×5 m for shrubs and 10 m×10 m for trees.All the data of 195 plant species and environmental factors were analyzed via Two-way Cluster Analysis and Canonical Correspondence Analysis using PCORD and CANOCO software.Among 195 plant species from 63 families were 27 species of Asteraceae,the most-speciose family,followed by Poaceae(20 spp.).The dominant life form was therophyte(86 spp.;44%share),followed by phanerophyte(41 spp.;21%share).For the leaf size spectrum,most prevalent was nanophyll(73 spp.;44%)followed by microphyll(66 spp.,34%)and mesophyll(44 spp.;23%).Calcium carbonate,pH,potassium,sand,silt,organic matter,slope aspect and grazing significantly influenced species composition,distribution and habitat.The floristic variation in the region was diverse due to elevational and aspect gradients and thus devided into three zones.This vegetation zonation can be utilized for forest management,species and habitat conservation.Both in situ and ex situ conservation of threatened plant species may improve their conservation status in future if address properly.  相似文献   

Comparison of protection and viral shedding following vaccination with Newcastle disease virus strains of different genotypes used in vaccine formulation     

Wajid  Abdul  Basharat  Asma  Bibi  Tasra  Rehmani  Shafqat Fatima 《Tropical animal health and production》2018,50(7):1645-1651

Tropical Animal Health and Production - Newcastle disease (ND) is a devastating disease and cause high t mortality and morbidity in poultry and nonpoultry avian species worldwide. An intensive...  相似文献   

Follicular dynamics and changes in oestradiol‐17β, progesterone and LH profiles following PGF2α induced oestrus in early and late ovulating Beetal goats     

Ali Murtaza  Waqas Ahmad  Tariq Sohail  Muhammad Irfan‐ur‐Rehman Khan  Imran Mohsin  Muhammad Shahzad  Mujahid Hussain  Muhammad Zahid Tahir  Muhammad Ijaz 《Reproduction in domestic animals》2019,54(3):545-550

This study compares the factors associated with variable interval to oestrus and ovulation between early versus late ovulating goats following PGF_2α administration. The time of ovulation in Beetal goats (n = 38) was monitored through transrectal ultrasound at every 6 hr following a single dose of PGF_2α (experiment 1). Variations in oestrus and ovulation times were further explored through the changes in follicular dynamics, endocrine profiles and behaviour in another set of goats (n = 13) following single PGF_2α given randomly during the luteal phase (experiment 2). The ovulation time varied between 60 and 96 hr, and 57% of ovulations occurred by 72 hr following PGF_2α (experiment 1). Accordingly, the goats (n = 13) in the second experiment were retrospectively divided either into early and/or late ovulating, that is, ≤72 and/or ≥84 hr following PGF_2α. The onset of oestrus, peak estradiol‐17β concentration and LH surge after PGF_2α was first observed in early than late ovulating goats (p < 0.05). The goats ovulating early had larger follicle and smaller CL in diameter at the time of PGF_2α administration than those ovulating late (5.4 ± 0.2 vs. 4.3 ± 0.2 mm and 10 ± 0.6 vs. 11.8 ± 0.3 mm, respectively; p < 0.05). Likewise, plasma progesterone concentration tended to be lower (p = 0.087) in early than late ovulating goats. In conclusion, the size of dominant follicle and CL at the time of PGF_2a determines the interval to ovulation following a single dose of PGF_2a during the luteal phase.  相似文献   

The first report on serotyping of bluetongue virus in small ruminants of Khyber Pakhtunkhwa province,Pakistan     

Malik  Amir Iftikhar  Ijaz  Muhammad  Yaqub  Tahir  Shabir  Muhammad Zubair  Avais  Muhammad  Ghaffar  Awais  Ali  Ahmad  Farooqi  Shahid Hussain  Mehmood  Khalid 《Tropical animal health and production》2019,51(4):977-982

Bluetongue virus (BTV), a member of Orbivirus genus (family Reoviridae), is a non-contagious infection of domestic and wild ruminants. The current study was designed to detect various serotypes of BTV in small ruminants of Khyber Pakhtunkhwa (KPK) province of Pakistan, along with their effects on hemato-biochemical parameters. A total of 408 serum samples in four districts (Mansehra, Abbottabad, Swabi, and Kohat) of KPK from small ruminants were screened based on competitive ELISA (cELISA). A total of 204 (50%) samples were found positive for BTV group–specific antibodies. The seropositive samples were processed for the detection of BTV serotypes through real-time polymerase chain reaction (qPCR). Out of 204 cELISA-positive samples, 60 (29.41%) were found positive through qPCR. Three serotypes [6, 8, 9] were detected from Mansehra District and two from Kohat [2, 8] and Abbottabad [6, 8], while only one from Swabi [8]. The serotype “8” was found consistently in all the four study districts. A significant (p?<?0.05) increase in the level of blood urea nitrogen (BUN) and alkaline phosphatase (ALP) was recorded in goats, whereas aspartate aminotransferase (AST) in sheep infected with BTV, compared to healthy animals. The hematological parameters showed significantly (p?<?0.05) raised total leucocyte count (TLC) in both sheep and goats, whereas only hematocrit (HCT) value was increased significantly (p?<?0.05) in infected sheep. This is the first report on serotyping of BTV among small ruminants in Pakistan.

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小黑麦基础日粮对育肥猪生产性能和猪肉品质的影响     

Zeb Sullivan  舒金秀  曾勇 《广东饲料》2008,17(7):42-42

小黑麦是硬质小麦和黑麦杂交的一种谷物,粗蛋白质含较量较玉米高,且其氨基酸组成更能满足育肥猪的氨基酸需要。与玉米基础饲粮相比,用小黑麦作为猪的饲粮,可以减少豆粕的用量。有关饲喂小黑麦对育肥猪的影响的研究结果并不一致,有研究报道当小黑麦代替玉米作为猪的日粮谷物源时,生产性能与玉米饲粮组相近,然而也有报道使其生产性能降低。  相似文献