Effect of Progesterone Prior to GnRH-PGF_2α Treatment on Induction of Oestrus and Pregnancy in Anoestrous Awassi Ewes     

MQ Husein  RT Kridli 《Reproduction in domestic animals》2003,38(3):228-232

An experiment was conducted to examine the effect of progesterone prior to a GnRH‐PGF_2α treatment on oestrus and pregnancy in seasonally anoestrous Awassi ewes. Twenty‐four ewes were randomly assigned to three groups to be pre‐treated with 60 mg medroxyprogesterone acetate sponges (group A), 600 mg progesterone sponges (group B) or blank sponges (group C) for 4 days. All ewes were injected with 100 μg of GnRH 24 h after sponge removal followed, 5 days later, by 20 mg PGF_2α injection. Ewes were exposed to three fertile rams at the time of PGF_2α injection (day 0, 0 h) and were checked for breeding marks at 6‐h intervals for 5 days. Blood samples were collected from all ewes 1 day (day ?10) prior to sponge insertion, at the time of sponge removal (day ?6), 1 day following sponge removal (day ?5, at the time of GnRH injection) and at the time of PGF_2α injection (day 0) for analysis of progesterone. Progesterone concentrations on days ?10 and ?5 were basal and averaged 0.2 ± 0.04 and 0.2 ± 0.2 ng/ml, respectively. Progesterone concentrations on day ?6 were elevated only in group B ewes and were higher (p < 0.0001) than those of groups A and C. Progesterone concentrations on day 0 were higher (p = 0.002) in groups A and B than group C. Oestrous responses occurred only in ewes of groups A and B (p > 0.05). Induced oestrus conception rate was greater (p < 0.01) in group A than groups B and C. Ewes returned to oestrus 17–20 days following day 0 were two of eight, six of eight and three of eight of groups A, B and C, respectively, all of which eventually lambed. The overall lambing rate was 82% in progesterone‐primed ewes compared with only 38% non‐progesterone‐primed ewes (p < 0.05). Progesterone priming apparently sensitizes GnRH‐PGF_2α‐treated seasonally anoestrous ewes and increases their response in oestrus and pregnancy rates.  相似文献   

The duration of lot-feeding of sheep before sea transport     

RT NORRIS  RB RICHARDS  GJ NORMAN 《Australian veterinary journal》1992,69(1):8-10

Adult wethers (n = 750) were lot-fed for 13 days, 8 days or 3 days before a simulated voyage lasting 18 days to examine whether the period of lot-feeding affected the proportion of sheep that ate pelleted feed and their body weight change during simulated shipping. There was no significant difference in the proportion of non-feeders between treatment groups on days 7 and 14 of the voyage. Body weights were not significantly different between the treatment groups on days 14 and 18 of the voyage. Overall body weight loss, from the farm to the end of simulated shipping, was 4.08 kg (+/- 0.28, s.e.m.), 4.58 kg (+/- 0.28) and 4.51 kg (+/- 0.28) in sheep lot-fed for 13 days, 8 days and 3 days, respectively, and was not significantly different between treatments. It was concluded that lot-feeding for 13 days conferred no advantage in body weight or numbers of non-feeders compared with shorter periods in this study.  相似文献   

Thrombocyte aggregation in the cat     

S Hart  I Nolte 《Tier?rztliche Praxis》1991,19(4):413-418

Platelet aggregation in healthy and sick cats after adding various aggregating agents is described. Feline platelets aggregate irreversibly in response to 0.15-1.0 micrograms/ml collagen, 1 microM ADP, 0.3 IU/ml test-thrombin and 0.71 NIH/ml Topostasin. Epinephrine, ristocetin and kaolin failed to cause aggregation. The aggregation function was decreased in a cat with liver damage and icterus; in 2 cats with uremia platelet aggregation was normal. Acetylsalicylic acid (ASA) (10 mg/kg iv) inhibits platelet aggregation in the presence of collagen in low concentrations; high concentrations of collagen succeeded in inducing platelet aggregation.  相似文献   

The effectiveness of the combined treatment of thrombocytic hemorrhagic diathesis of dogs with prednisolone and blood transfusion in the model of an aspirin-produced thrombocytopathy     

I Nolte  C Niemann  K Failing  G Müller-Berghaus 《Tier?rztliche Praxis》1988,16(4):417-422

The administration of 20 mg/kg of acetylsalicylic acid in 18 clinically healthy dogs resulted in a thrombocytopathy with lengthened capillary bleeding time and irreversible aggregation inhibition. Through the set up of individual dilution series, one could conclude the proportional percentage of aggregation functional transfused thrombocytes. The capillary bleeding time was shortened after the intravenous injection of prednisolone (5 mg/kg) without measurable influence on the thrombocytes. Compared to the singular use of cortisone or blood transfusion alone, the effect on capillary bleeding time became magnified when one combined transfusion and corticosteroids. The fresh blood conserves (12 hours) were, with respect to the haemostyptical properties, superior to blood stored for 5 days. The component of aggregation-functional thrombocytes was, due to the prophylactic cortisone application prior to transfusion, not increased.  相似文献   

Complications after implantation of a modular total hip prostheses compared with fixed head prostheses     

Gervers M  Nolte I  Alt F  Hauschild G  Meyer-Lindenberg A  Fehr M 《Berliner und Münchener tier?rztliche Wochenschrift》2002,115(11-12):412-419

164 total hip prostheses of different manufacturers were implanted over an 8-year period. Clinical records were evaluated with a complication rate of 29.0% (42 of 145 joints) or 31.7% (39 of 123 dogs). The complications encountered in dogs that received the modular hip prosthesis (Biomécanique, Bretigny-sur-orge, France) amounted to 16.3% (15 of 92), dogs that received a fixed head prosthesis (5 different manufactures) had 39% complications (28 of 72). Although the complication rate was influenced by different surgeons (n = 5) with different system predelictions and a different learning curve it was obvious that luxations (n = 6 of 10) and stem fractures (n = 5) more often occurred with the fixed head prosthesis systems. These stem fractures might be due to implant material failure or due to improper cementation followed by fatigue material failure. Femur fracture or infection were encountered with both prosthesis systems.  相似文献   

Possibilities and uses of flow cytometry in the framework of clinical diagnostics in dogs and cats     

Culmsee K  Nolte I 《DTW. Deutsche tier?rztliche Wochenschrift》2000,107(12):481-485

Flow cytometry has become a routine method in human medicine in the last years. Important applications in clinical diagnostics are measurement of DNA-content, cell cycle analysis, immunophenotyping of lymphocytes and cell function measurements. Flow cytometry is at present rarely applied in veterinary medicine. This article reviews basic principles of flow cytometry and demonstrates possibilities of flow cytometric analysis in oncology and diagnostics of anaemia and immunodeficiency in dogs and cats.  相似文献   

Management of inappetant sheep during export by sea     

RT NORRIS  CL McDONALD  RB RICHARDS  MW HYDER  SP GITTINS  GJ NORMAN 《Australian veterinary journal》1990,67(7):244-247

In the first of 2 experiments, a simulated voyage was conducted to examine the effects of various treatments on bodyweight change and feeding frequency of inappetant sheep at the end of lot-feeding (non-feeders). The treatments, applied during simulated shipping, were: normal quantities of feed and length of troughs; extra trough length; extra feed. Adult Merino wethers (n = 108) were used in each treatment. A voyage to the Middle East was then conducted to establish whether shipboard mortality could be reduced by separating non-feeders (n = 305) from feeders (n = 5,620) late in the feedlot hase and housing the groups separately aboard ship. A control group of non-feeders (n = 215) mixed with feeders (n = 5,732) was used for comparison. Bars (marker bars), containing a coloured dye, were attached to feed troughs to mark sheep that fed. Most non-feeders (82%) began eating when placed in shipping pens in both studies. However, there was no significant difference in percentage of sheep that fed between non-feeders given extra trough length or extra feed compared with non-feeders given standard management at any stage of simulated shipping. There was no significant difference in mean bodyweights between treatment groups on days 1, 8 and 15 of simulated shipping. Differences in bodyweight on d 22 were probably associated with different levels of gut fill. Death rates were not significantly different in separated and control groups (1.1%, 0.9%, P = 0.6) in the voyage of 14 d to the Middle East.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Beobachtungen anläъlich des Massenauftretens der Ypsiloneule (Rhyacia = Agrotis ypsilon Rott.) im Sommer 1952 in Mitteldeutschland     

H. -W. Nolte  R. Fritzsche 《Journal of pest science》1953,26(3):33-35

Zusammenfassung Im Sommer 1952 wurden an verschiedenen Stellen in Mitteldeutschland schwere Schäden durch Erdraupen festgestellt. Die Artbestimmung ergab, daß es sich um eine Massenvermehrung der Ypsiloneule (Rhyacia ypsilon Rott.) handelte. Schäden wurden an Kartoffeln, Zuckerrüben, Mohrrüben, Mohn, Tabak, Majoran und Gladiolen beobachtet. Die Schadbilder werden genauer beschrieben. Als Ursache für das Massenauftreten wird das Zusammentreffen von zwei Generationen angenommen, von denen die eine als von überwinterten Faltern stammend angesehen werden muß. Die Massenvermehrung dürfte daher eine Folge des anormal warmen Winters 1951/52 sein. Als Parasit wurdeMacrocentrus collaris Spin. festgestellt.  相似文献   

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND) progress to date and future plans     

Charlotte M. Keenan  Julia F. Baker  Alys E. Bradley  Dawn G. Goodman  Takanori Harada  Ronald Herbert  Wolfgang Kaufmann  Rupert Kellner  Beth Mahler  Emily Meseck  Thomas Nolte  Susanne Rittinghausen  John Vahle  Katsuhiko Yoshizawa 《Journal of toxicologic pathology》2015,28(1):51-53

The INHAND Proposal (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) has been operational since 2005. A Global Editorial Steering Committee (GESC) manages the overall objectives of the project and the development of harmonized terminology for each organ system is the responsibility of the Organ Working Groups (OWG), drawing upon experts from North America, Europe and Japan.Great progress has been made with 9 systems published to date – Respiratory, Hepatobiliary, Urinary, Central/Peripheral Nervous Systems, Male Reproductive and Mammary, Zymbals, Clitoral and Preputial Glands in Toxicologic Pathology and the Integument and Soft Tissue and Female Reproductive System in the Journal of Toxicologic Pathology as supplements and on a web site – www.goreni.org. INHAND nomenclature guides offer diagnostic criteria and guidelines for recording lesions observed in rodent toxicity and carcinogenicity studies. The guides provide representative photo-micrographs of morphologic changes, information regarding pathogenesis, and key references. During 2012, INHAND GESC representatives attended meetings with representatives of the FDA Center for Drug Evaluation and Research (CDER), Clinical Data Interchange Standards Consortium (CDISC), and the National Cancer Institute (NCI) Enterprise Vocabulary Services (EVS) to begin incorporation of INHAND terminology as preferred terminology for SEND (Standard for Exchange of Nonclinical Data) submissions to the FDA. The interest in utilizing the INHAND nomenclature, based on input from industry and government toxicologists as well as information technology specialists, suggests that there will be wide acceptance of this nomenclature. The purpose of this publication is to provide an update on the progress of INHAND.  相似文献   

Atomic structure of PDE4: insights into phosphodiesterase mechanism and specificity     

Xu RX  Hassell AM  Vanderwall D  Lambert MH  Holmes WD  Luther MA  Rocque WJ  Milburn MV  Zhao Y  Ke H  Nolte RT 《Science (New York, N.Y.)》2000,288(5472):1822-1825

Cyclic nucleotides are second messengers that are essential in vision, muscle contraction, neurotransmission, exocytosis, cell growth, and differentiation. These molecules are degraded by a family of enzymes known as phosphodiesterases, which serve a critical function by regulating the intracellular concentration of cyclic nucleotides. We have determined the three-dimensional structure of the catalytic domain of phosphodiesterase 4B2B to 1.77 angstrom resolution. The active site has been identified and contains a cluster of two metal atoms. The structure suggests the mechanism of action and basis for specificity and will provide a framework for structure-assisted drug design for members of the phosphodiesterase family.  相似文献