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排序方式: 共有130条查询结果,搜索用时 31 毫秒
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Experimental infection of normal and immunosuppressed pigs with Pseudomonas pseudomallei 总被引:1,自引:0,他引:1
AD THOMAS JC FORBES-FAULKNER TL D''ARCY JH NORTON D HOFFMANN 《Australian veterinary journal》1990,67(2):43-46
A single dose of 5 x 10(8) bacilli of Pseudomonas pseudomallei by intratracheal injection resulted in acute (21 cases) or chronic (19 cases) melioidosis in 40 of 48 pigs. Fifteen (10 acute and 5 chronic) had been immunosuppressed by cyclophosphamide before inoculation. The major clinical signs were initial fever, marked neutrophilia and, in the acute cases, respiratory distress. There were no signs of the nasal and ocular discharge, paresis or diarrhoea seen in acute cases in south-east Asia. The cyclophosphamide treatment caused a significant decrease in the neutrophil count by 7 d after inoculation in all 15 immunosuppressed pigs, and all were culture positive at necropsy. Eight of the 33 non-treated pigs were culture negative at necropsy. Pigs overcoming the initial phase of infection had more abscess-like nodules that were bacteriologically sterile at necropsy than the pigs with acute cases of melioidosis. P. pseudomallei was isolated predominantly from the spleen, lungs and the injection site. Although only one strain was used in this study, it is likely that Australian strains of P. pseudomallei are not as virulent as the south-east Asian isolates. 相似文献
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Closure of the Newfoundland commercial Atlantic salmon, Salmo salar L., fishery in 1992 was the most restrictive measure introduced to help rebuild depressed local stocks of salmon. Here, the effects of the closure are evaluated by analysing trends in abundance since 1984, and estimates of survival in both freshwater and marine environments derived from enumeration of salmon at fish counting facilities. While freshwater production of smolts generally has been maintained, marine survival rates remain low (2–10%), and highly variable. Overall, total stock size differs little from that prior to the closure of the commercial salmon fishery. Spawning escapements have increased by a factor of 2 or 3 in some rivers, but in other areas total returns are lower on average than those prior to the fishery closure. Factors other than exploitation are contributing to lack of stock recovery, resulting in continued conservation concerns. 相似文献
5.
M. F. O'Connell 《Fisheries Management and Ecology》2003,10(4):201-208
Conservation spawning requirements (limits) for Atlantic salmon, Salmo salar L., have been developed for a number of rivers in Newfoundland. Status of stocks is evaluated against these limits and scientific advice and recommendations on levels of recreational catch provided to resource managers. A critical factor in this process is the determination of total returns. This has been accomplished for 30–40% of the rivers in question over the years through the use of fish counting fences and traps installed in fishways. In Newfoundland, it is not logistically or financially possible to operate fish counting facilities in all of the approximately 200 Atlantic salmon rivers. Thus angling catch data have been used as an alternative means of estimating total returns to rivers without counting facilities. Estimates of total returns were three to four times higher than the actual values for one of the two rivers studied and deviated from the actual by as much as 60% for the other. Management implications of the approach are discussed. 相似文献
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Anne-marie Wolters Evert Jacobsen Mary O'Connell Guusie Bonnema K. Sree Ramulu Hans de Jong Herman Schoenmakers Jelle Wijbrandi Maarten Koornneef 《Euphytica》1994,79(3):265-277
Protoplast fusion can be used to produce somatic hybrids of species that cannot be obtained by sexual hybridization. The possibility to introgress genes from Solanum species into the cultivated tomato species Lycopersicon esculentum, and to obtain novel cytoplasm-nucleus combinations (cybrids) was considered as an important strategy to extend the genetic variation available for tomato breeding. Somatic hybrids between L. esculentum and other Lycopersicon species, as well as between L. esculentum and Solanum or Nicotiana species, have been produced. Specific mutants, genotypes with antibiotic resistances, and metabolic inhibition by iodoacetate or iodoacetamide and irradiation were used for the selection of hybrids. In addition, the improvement of protoplast culture techniques and the use of the favourable tissue culture traits derived from species such as L. peruvianum, which have been introduced into tomato by classical breeding, allowed the efficient recovery of somatic hybrids. However, the occurrence of somatic incongruity in fusion combinations of L. esculentum and Solanum and even more in L. esculentum and Nicotiana, did not allow the production of true cybrids and/or fertile hybrids, indicating the importance of both cytoplasm-nucleus and nucleus-nucleus interactions in somatic incongruity. Another problem with fusions between distantly related species is the strongly reduced fertility of the hybrids and the very limited homoeologous recombination between chromosomes of the parental species. Partial genome transfer from donor to recipient through microprotoplast (+) protoplast fusion, and the production of monosomic or disomic chromosome addition lines, light overcome some of these problems. In symmetric somatic hybrids between L. esculentum and S. tuberosum the occurrence of limited somatic and meiotic recombination was demonstrated. Fertile progeny plants could be obtained, though at a low frequency, when embryo rescue was performed on a large scale after backcrossing hexaploid somatic tomato (+) potato hybrids with a tetraploid potato genotype. The potential value of genomic in situ hybridization (GISH) and RFLPs for the analysis of the genome/chromosome composition of the hybrids has been demonstrated for intergeneric somatic hybrids between Lycopersicon and Solanum.Abbreviations cpDNA
chloroplast DNA
- mtDNA
mitochondrial DNA 相似文献
8.
Natural variation of canine parvovirus 总被引:20,自引:0,他引:20
C R Parrish P H O'Connell J F Evermann L E Carmichael 《Science (New York, N.Y.)》1985,230(4729):1046-1048
Canine parvovirus was first recognized during 1978. Analysis of isolates collected since its emergence revealed that viruses circulating after 1980 were antigenically different from earlier isolates. Monoclonal antibodies clearly distinguished the two strains, some being specific for either the old or the new viruses. Restriction enzyme analysis of viral DNA's showed that the post-1980 viruses were similar to earlier isolates, but some restriction site differences were present in the new strain. These results suggest that the canine parvoviruses infecting dogs in the seven areas of the United States that were sampled derive from a variant virus that replaced the original strain during 1980. 相似文献
9.
O'Connell MJ Bachilo SM Huffman CB Moore VC Strano MS Haroz EH Rialon KL Boul PJ Noon WH Kittrell C Ma J Hauge RH Weisman RB Smalley RE 《Science (New York, N.Y.)》2002,297(5581):593-596
Fluorescence has been observed directly across the band gap of semiconducting carbon nanotubes. We obtained individual nanotubes, each encased in a cylindrical micelle, by ultrasonically agitating an aqueous dispersion of raw single-walled carbon nanotubes in sodium dodecyl sulfate and then centrifuging to remove tube bundles, ropes, and residual catalyst. Aggregation of nanotubes into bundles otherwise quenches the fluorescence through interactions with metallic tubes and substantially broadens the absorption spectra. At pH less than 5, the absorption and emission spectra of individual nanotubes show evidence of band gap-selective protonation of the side walls of the tube. This protonation is readily reversed by treatment with base or ultraviolet light. 相似文献
10.
Hypertriglyceridemia as a result of human apo CIII gene expression in transgenic mice 总被引:24,自引:0,他引:24
Primary and secondary hypertriglyceridemia is common in the general population, but the biochemical basis for this disease is largely unknown. With the use of transgenic technology, two lines of mice were created that express the human apolipoprotein CIII gene. One of these mouse lines with 100 copies of the gene was found to express large amounts of the protein and to be severely hypertriglyceridemic. The other mouse line with one to two copies of the gene expressed low amounts of the protein, but nevertheless manifested mild hypertriglyceridemia. Thus, overexpression of apolipoprotein CIII can be a primary cause of hypertriglyceridemia in vivo and may provide one possible etiology for this common disorder in humans. 相似文献