The surgical correction of a deviated anterior maxilla in a horse     

GMW McKELLAR  AP COLLINS 《Australian veterinary journal》1993,70(3):112-114

SUMMARY The surgical correction of facial deformities of the horse have rarely been undertaken. The surgical and medical management of submucous clefting of the anterior maxilla in a young colt is described.  相似文献   

Influence of glucosamine on matrix metalloproteinase expression and activity in lipopolysaccharide-stimulated equine chondrocytes     

Byron CR  Orth MW  Venta PJ  Lloyd JW  Caron JP 《American journal of veterinary research》2003,64(6):666-671

OBJECTIVE: To characterize potential mechanisms of action of glucosamine inhibition of matrix metalloproteinase (MMP) expression and activity in lipopolysaccharide (LPS)-stimulated equine chondrocytes. SAMPLE POPULATION: Chondrocytes cultured from samples of metacarpophalangeal articular cartilage collected from cadaveric limbs of horses. PROCEDURE: The effect of glucosamine on MMP activity in conditioned medium from LPS-stimulated cartilage explants was determined by a colorimetric assay with azocoll substrate. Treatments consisted of negative and positive controls, glucose (50 mM), and glucosamine (50, 25, 6.25, 3, and 1.5 mM). The influence of glucosamine on MMP synthesis was determined in chondrocytes in pellet culture incubated with LPS (20 microg/mL). Concentration of MMP-13 was quantified in spent medium via ELISA; nonspecific MMP activity was determined via azocoll digestion in organomercurial-activated medium. Effects of glucosamine on MMP mRNA concentration in similarly treated chondrocytes were determined by northern blot hybridization with MMP-1, -3, and -13 probes. Statistical analyses were performed with 2-way ANOVA. RESULTS: Glucosamine had no effect on activated MMP activity but inhibited MMP protein expression, as determined by azocoll digestion (glucosamine, 3 to 50 mM) and MMP-13 ELISA (glucosamine, 1.5 to 50 mM). Resting mRNA concentrations for MMP-1, -3, and -13 mRNA were significantly lower in cultures exposed to glucosamine at concentrations of 50 and 25 mM than those of positive controls. CONCLUSIONS AND CLINICAL RELEVANCE: Glucosamine appears capable of pretranslational, and possibly also translational, regulation of MMP expression; data suggest a potential mechanism of action for chondroprotective effects of this aminomonosaccharide.  相似文献   

Serum concentrations of keratan sulfate,osteocalcin, and pyridinoline crosslinks after oral administration of glucosamine to standardbred horses during race training     

Caron JP  Peters TL  Hauptman JG  Eberhart SW  Orth MW 《American journal of veterinary research》2002,63(8):1106-1110

OBJECTIVE: To determine the effects of orally administered glucosamine on concentrations of markers of bone and cartilage metabolism in Standardbred horses during race training. ANIMALS: Twenty 16- to 20-month-old Standardbreds beginning race training. PROCEDURE: Horses were randomly assigned to 2 groups. One group received glucosamine hydrochloride (4 g, PO, q 12 h), and the second (control) group received glucose (4 g, PO, q 12 h). Serum samples were obtained prior to onset of the study (baseline) and at regular intervals for 48 weeks for determination of concentrations of keratan sulfate (KS), osteocalcin (OC), and pyridinoline crosslinks (PYD). RESULTS: Osteocalcin concentrations changed significantly with time; mean serum concentrations were significantly higher than baseline values for samples obtained at 24 to 48 weeks after onset of the study. Although a significant effect of time was observed for mean concentration of KS, concentrations did not differ significantly from baseline values at any time during the study when groups were analyzed separately. However, pooled analysis revealed significant increases of mean serum KS concentration at weeks 24 and 30. Significant changes in serum PYD concentrations were not detected. Oral administration of glucosamine did not significantly affect serum concentrations of any of the markers. CONCLUSIONS AND CLINICAL RELEVANCE: Increased serum OC in clinically normal Standardbreds during race training may reflect bone formation that accompanies adaptive remodeling of the appendicular skeleton. For these experimental conditions, glucosamine did not appear to exert a detectable influence on serum concentrations of these 3 markers of connective tissue metabolism.  相似文献   

Suspected salmonellosis in seven broodmares after transportation     

SA McCLINTOCK  AP BEGG 《Australian veterinary journal》1990,67(7):265-267

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Adenylyl cyclase amino acid sequence: possible channel- or transporter-like structure   总被引：47，自引：0，他引：47  

J Krupinski  F Coussen  H A Bakalyar  W J Tang  P G Feinstein  K Orth  C Slaughter  R R Reed  A G Gilman 《Science (New York, N.Y.)》1989,244(4912):1558-1564

Complementary DNA's that encode an adenylyl cyclase were isolated from a bovine brain library. Most of the deduced amino acid sequence of 1134 residues is divisible into two alternating sets of hydrophobic and hydrophilic domains. Each of the two large hydrophobic domains appears to contain six transmembrane spans. Each of the two large hydrophilic domains contains a sequence that is homologous to a single cytoplasmic domain of several guanylyl cyclases; these sequences may represent nucleotide binding sites. An unexpected topographical resemblance between adenylyl cyclase and various plasma membrane channels and transporters was observed. This structural complexity suggests possible, unappreciated functions for this important enzyme.  相似文献   

A strategy for probing the function of noncoding RNAs finds a repressor of NFAT   总被引：3，自引：0，他引：3  

Willingham AT  Orth AP  Batalov S  Peters EC  Wen BG  Aza-Blanc P  Hogenesch JB  Schultz PG 《Science (New York, N.Y.)》2005,309(5740):1570-1573

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An iridium abundance anomaly at the palynological cretaceous-tertiary boundary in northern new Mexico     

Orth CJ  Gilmore JS  Knight JD  Pillmore CL  Tschudy RH  Fassett JE 《Science (New York, N.Y.)》1981,214(4527):1341-1343

An iridium abundance anomaly, with concentrations up to 5000 parts per trillion over a background level of 4 to 20 parts per trillion, has been located in sedimentary rocks laid down under freshwater swamp conditions in the Raton Basin of northeastern New Mexico. The anomaly occurs at the base of a coal bed, at the same stratigraphic position at which several well-known species of Cretaceous-age pollen became extinct.  相似文献   

Geologic framework of nonmarine cretaceous-tertiary boundary sites, raton basin, new Mexico and colorado     

Pillmore CL  Tschudy RH  Orth CJ  Gilmore JS  Knight JD 《Science (New York, N.Y.)》1984,223(4641):1180-1183

Iridium concentrations are anomalously high at the palynological Cretaceous-Tertiary boundary in fluvial sedimentary rocks of the lower part of the Raton Formation at several localities in the Raton Basin of New Mexico and Colorado. The iridium anomaly is associated with a thin bed of kaolinitic claystone in a discontinuous carbonaceous shale and coal sequence.  相似文献   

Fortgesetzte Untersuchungen zur Bekämpfung der Bohnenfliegen     

H. Bremer  H. Orth 《Journal of pest science》1955,28(9):138-140

Zusammenfassung Die beste und billigste Abwehr gegen Bohnenfliegenschaden besteht zur Zeit in der Saatgutbeizung mit einem Insektizid und Fungizid enthaltenden Beizmittel. Bei Verwendung eines Anklebemittels zum Saatgut kann und muß die Beizmittelmenge auf die Hälfte herabgesetzt werden. Die Saatgutbeizung lohnt sich bei Aussaat zu verschiedenen Zeiten der Vegetationsperiode.  相似文献   

Iodometric analysis and shelf life of malathion in formulations     

Robert A. Orth  William Minett 《Pest management science》1975,6(3):217-221

Using an iodometric method for the determination of malathion in emulsifiable concentrates, values of active ingredient in four commercial formulations and one primary reference standard were within ±0.7% weight/volume (w/v) of the stated concentrations. A standard deviation of 0.42% w/v and 95% confidence limits of ±0.9% w/v for a single determination were calculated for the method. Added moisture and elevated temperature were found to accelerate malathion breakdown in the commercial formulations. The greatest breakdown rate of 5.2% w/v per 100 days occurred with a formulation stored at 27 °C containing 0.5% v/v added moisture.  相似文献