Production of primary cell cultures on a semi-industrial scale. 1. Modification of the trypsinization procedure--possible means to improve cell yield     

R Beyer  M Rex 《Archiv fuer experimentelle veterinaermedizin》1979,33(4):499-512

To produce large quantities of primary cell suspensions on efficient technique is described which is named "Biomix-Method". By this technique first tissue mince is performed automatically using tissue amounts between 300 g and 500 g. Trypsinization takes place in a three-litre trypsinization vessel using a stirring mechanism according to a defined trypsinization mode. In regard to optimal cell seeding concentration and a justifiable productivity one kidney of newborn or suckling calf yields 6--9 1 cell suspension and that of piglet 1,8-2,3 1, respectively. Proliferation behaviour and effectivity of primary cells are discussed and compared with other cell systems.  相似文献   

AUSTRALIAN COLLEGE OF VETERINARY SCIENTISTS TENTH ANNUAL GENERAL MEETING 1980     

M. A. E. Rex 《Australian veterinary journal》1981,57(1):43-44

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Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs     

Juliana Abranches Soares Almeida  Yanhong Liu  Minho Song  Jeong Jae Lee  H Rex Gaskins  Carol Wolfgang Maddox  Orlando Osuna  James Eugene Pettigrew 《畜牧与生物技术杂志(英文版)》2014,(2):173-180

An experiment was conducted to determine how an E. coil challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs （initial BW： 6.9 ± 1.0 kg） were distributed in a 2 x 4 factorial arrangement of a randomized complete block design （2 challenge treatments： sham or E. coil, and 4 dietary treatments： control, 0.3% smectite A, 0.3% smectite B and 0.3% zeolite）, with 8 replicates total. Diarrhea score, growth performance, goblet cell size and number, bacterial translocation from intestinal lumen to lymph nodes, intestinal morphology, and relative amounts of sulfo and sialo mucins were measured. The E. coli challenge reduced performance, increased goblet cell size and number in the ileum, increased bacterial translocation from the intestinal lumen to the lymph nodes, and increased ileal crypt depth. One of the clays （smectite A） tended to increase goblet cell size in ileum, which may indicate enhanced protection. In conclusion, E. coli infection degrades intestinal barrier integrity but smectite A may enhance it.  相似文献   

Induction of phase II detoxification enzymes in rats by plant-derived isothiocyanates: comparison of allyl isothiocyanate with sulforaphane and related compounds     

Munday R  Munday CM 《Journal of agricultural and food chemistry》2004,52(7):1867-1871

Plants of the family Brassicaceae contain high levels of glucosinolates. The latter compounds are degraded to isothiocyanates, some of which have been shown to be potent inducers of phase II detoxification enzymes in vitro. In the present study, the ability of six plant-derived isothiocyanates (allyl isothiocyanate, iberverin, erucin, sulforaphane, iberin, and cheirolin) to increase tissue levels of the phase II detoxification enzymes quinone reductase (QR) and glutathione S-transferase (GST) in a variety of rat tissues has been compared. At the low dose level employed (40 micromol/kg/day), cheirolin was without effect in any tissue. All of the other isothiocyanates, however, increased GST and QR activities in the duodenum, forestomach, and/or the urinary bladder of the animals, with the greatest effects being seen in the urinary bladder. With the exception of cheirolin, little difference was observed in the inductive activity of the various isothiocyanates. Phase II enzymes are known to protect against chemical carcinogenesis, and the selectivity of isothiocyanates in inducing such enzymes in the bladder is of interest in view of recent epidemiological studies showing a decreased incidence of cancer of this organ in individuals with a high dietary intake of Brassica vegetables.  相似文献   

Field Scale Mapping of Surface Soil Clay Concentration     

Chen  Feng  Kissel  David E.  West  Larry T.  Adkins  W.  Clark  Rex  Rickman  Doug  Luvall  J. C. 《Precision Agriculture》2004,5(1):7-26

The surface soil clay concentration is a useful soil property to map soils, interpret soil properties, and guide irrigation, fertilizer, and agricultural chemical applications. The objective of this study was to determine whether surface soil clay concentrations could be predicted from remotely sensed imagery of bare surface soil or from soil electrical conductivity for a 115 ha field located in Crisp County, Georgia. The soil clay concentrations were determined for soil samples taken at 28 field locations. Three different data sources–an aerial color photograph image, two infrared bands from an ATLAS data set, and the electrical conductivity of the surface soil layer were used in the research. Principal components analysis was applied to the color photograph image, whereas the ratio of two infrared bands was applied to the ATLAS data set. Filtering was applied to both resulting images. The distribution of soil electrical conductivity was derived from the measured soil electrical conductivity data by spatial analysis. Statistical relationships between soil clay concentrations and the principal component 3, the ratio of two ATLAS infrared bands, and the soil electrical conductivity were analyzed, and three linear equations were derived with r ² values 0.83, 0.52, and 0.78, respectively. The distribution of the soil clay concentrations was derived based on these three equations. Six levels of soil clay concentrations were classified in these three methods, and the advantages and disadvantages were discussed. The predicted and measured soil clay concentrations, based on additional soil samples from 30 field locations, were compared using linear regression (r ²=0.76, 0.45, and 0.77 for the three methods). The overall accuracy for these methods were 84%, 66%, and 76%, respectively. The principal components method had the highest accuracy in our research, while the result for the depressional areas is the best from the ratio method.  相似文献   

Hemolytic anemia and induction of phase II detoxification enzymes by diprop-1-enyl sulfide in rats: dose-response study     

Munday R  Munday CM  Munday JS 《Journal of agricultural and food chemistry》2005,53(25):9695-9700

Epidemiological evidence indicates that a high dietary intake of plants of the Allium family, such as garlic and onions, is associated with a decreased risk of cancer in humans. It has been suggested that this chemopreventative effect involves the ability of the aliphatic sulfides derived from these vegetables to increase tissue activities of phase II detoxification enzymes. Several highly effective inducers from garlic have been identified, but most of the previously studied compounds from onion have proved to be only weakly active. In the present study, the inductive activity of another onion-derived sulfide, diprop-1-enyl sulfide, has been investigated. This substance was a potent inducer of phase II enzymes in rats, showing significant effects in the lungs and in the lower part of the gastrointestinal tract, suggesting that diprop-1-enyl sulfide could be a useful chemopreventative agent at these sites. At high dose levels, diprop-1-enyl sulfide caused hemolytic anemia, which may be due to in vivo conversion of the sulfide to active metabolites.  相似文献   

CORRECTION     

Rex Butterfield 《Australian veterinary journal》1993,70(12):464-464

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An unusual sequel to intramedullary pinning     

M A Rex 《New Zealand veterinary journal》1965,13(2):45-46

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SPREULL'S EXCISION ARTHROPLASTY OF THE HIP JOINT IN THE DOG AND CAT     

M. A. E. Rex  M.A.  Vet.M.B.  M.R.C.V.S. 《Australian veterinary journal》1963,39(7):275-278

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SURGICAL TREATMENT OF THREE COMMON ORTHOPAEDIC CONDITIONS OF THE DOG'S STIFLE     

M. A. E. Rex  M.A.  Vet.M.B.  M.R.C.V.S. 《Australian veterinary journal》1963,39(7):268-274

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