Identification of irisin immunoreactivity in porcupine (Hystrix cristata) adrenal glands and kidneys          下载免费PDF全文

Sema Timurkaan  Fatih M. Gür  Berrin Gençer Tarakçı  Mehmet H. Yalçın  Mustafa Girgin 《Anatomia, histologia, embryologia》2018,47(5):405-409

Irisin, a novel peptide, was initially been shown to be expressed explicitly in the muscle tissues. We studied the presence of irisin immunoreactivity in porcupine adrenal glands and kidneys. Immunocytochemistry showed that irisin was localised both in the adrenal cortex and adrenal medulla. In organs, irisin immunoreactivity was found in the tubular and collecting system of the nephron. The functional role of irisin in the adrenal gland and kidney has not been precisely yet. However, irisin might have a paracrine and autocrine function as do other locally produced peptides.  相似文献   

Effects of 3-methyl-4-nitrophenol in diesel exhaust particles on the regulation of reproductive function in immature female Japanese quail (Coturnix japonica)     

Li C  Takahashi S  Taneda S  Furuta C  Watanabe G  Suzuki AK  Taya K 《The Journal of reproduction and development》2007,53(3):673-678

In a previous study, we found that 3-methyl-4-nitrophenol (PNMC), a component of diesel exhaust particles and also a degradation product of the insecticide fenitrothion, exhibits reproductive toxicity in the adult male Japanese quail. The present study investigated the toxicity of PNMC in the female Japanese quail and its ability to influence reproduction in immature females. The quail (21-day-old) were injected intramuscularly (im) with PNMC at doses 0.1, 1 or 10 mg/kg body weight daily for 3 days. There was no significant difference in body growth between the PNMC-administered and control birds. However, the weights of the oviducts were significantly lower in the PNMC-treated birds at all doses. Furthermore, the plasma concentrations of luteinizing hormone (LH) and estradiol-17 beta were significantly decreased with 1 and 10 mg/kg of PNMC. These findings suggest that PNMC might influence the hypothalamo-pituitary-gonadal axis with decreasing in secretion of GnRH, LH and ovarian steroid hormones and subsequently disturb growth of the reproductive organs of immature female quail. This study indicates that PNMC induces reproductive toxicity at the central level and disrupts reproductive function in the immature female quail.  相似文献   

Direct effects of prolactin on adrenal steroid release in male Hatano high-avoidance (HAA) rats may be mediated through Janus kinase 2 (Jak2) activity   总被引：1，自引：0，他引：1  

Jaroenporn S  Nagaoka K  Ohta R  Watanabe G  Taya K 《The Journal of reproduction and development》2007,53(4):887-893

Prolactin (PRL) has been proposed to directly stimulate corticosterone release. However, the role of PRL on adrenocortical function in male HAA rats has not been fully clarified. The aim of this study was to investigate the effect of PRL on the secretion of corticosterone and progesterone using an in vitro cell culture system in male rats. Administration of PRL (10(-7) and 10(-6) M) resulted in dose-dependent increases in corticosterone and progesterone release. Cotreatment with PRL produced an increase in the stimulatory effects of ACTH-induced corticosterone and progesterone secretion. However, the PRL-induced corticosterone and progesterone releases were significantly reduced by treatment with AG490, a specific Janus kinase 2 (Jak2) inhibitor. In addition, administration of AG490 blunted the significant inhibition of ACTH-induced corticosterone and progesterone secretion by PRL. These results demonstrated that PRL could act directly on the adrenal gland to drive corticosterone and progesterone secretion in male rats. Additionally, the results emphasize that PRL stimulation of adrenal steroid release may be mediated through Jak2 activity.  相似文献   

Assessment of fertility and reproductive toxicity in adult female mice after long-term exposure to Pueraria mirifica herb   总被引：1，自引：0，他引：1  

Jaroenporn S  Malaivijitnond S  Wattanasirmkit K  Watanabe G  Taya K  Cherdshewasart W 《The Journal of reproduction and development》2007,53(5):995-1005

The present study investigated the effects of long-term administration of Pueraria mirifica (PM) at non-toxic doses on the ovarian function and fertility of adult female mice based on evaluation of hematological and biochemical parameters. Female mice were divided into 4 groups (36 mice/group). Groups 1-3 were orally treated with a dose of 0 (PM-0), 10 (PM-10) or 100 mg/kg BW/day PM (PM-100), and group 4 was subcutaneously injected with 200 mug/kg BW/day of synthetic estrogen diethylstilbestrol (DES). The treatment schedule was separated into treatment and post-treatment periods. The duration of each period was 8 weeks. The PM-10 mice exhibited regular estrous cycles, while the PM-100 and DES treatments induced prolonged estrous cycles. Although no changes were observed in the uterus and ovary weights of the mice after the PM-100 and DES treatments, hyperplasia of the uterine endothelium and a decrease in the number of growing ovarian follicles were detected. The changes in the ovarian histologies of the PM-100 and DES mice were related to reductions in the levels of LH and FSH, which subsequently caused a decrease in mating efficiency. Once the PM mice were able to copulate, they were capable of successfully becoming pregnant and mothering offspring. No abnormalities were observed in the external morphologies and reproductive organ weights of the 50-day-old offspring. In conclusion, our results suggest that long-term exposure to 100 mg/kg BW of PM has adverse effects on the mating efficiency and reproduction of adult female mice and that administration of 10 mg/kg BW of PM does not induce any changes in the hypothalamic-pituitary-ovarian-uterine axis.  相似文献   

Effects of diesel exhaust particles on the male reproductive system in strains of mice with different aryl hydrocarbon receptor responsiveness   总被引：1，自引：0，他引：1  

Izawa H  Kohara M  Watanabe G  Taya K  Sagai M 《The Journal of reproduction and development》2007,53(6):1191-1197

Diesel exhaust particles (DEPs) contain polycyclic aromatic hydrocarbons (PAH) that bind to aryl hydrocarbon receptors (AhRs) and decrease sperm production. Since it is not clear if AhR mediates DEP toxicity, we investigated the effect of DEPs in four strains of mice that have different AhR responsiveness. We treated BALB/c, C57BL/6, ICR and DBA/2 mice with DEP suspensions and compared their toxicity in each strain. In both the vehicle- and DEP-treated groups, ethoxyresorufin-O-deethylase (EROD) activity, as an indirect index of AhR activity, was increased in the order of BALB/c > C57BL/6 > ICR > DBA/2. Only BALB/c and C57BL/6 mice had significantly lower daily sperm production (DSP) than vehicle-treated mice. All strains exhibited increased sperm abnormalities. In particular, the C57BL/6, ICR and DBA/2 mice exhibited significantly increased abnormalities. A significant correlation was found between EROD activity and DSP or incidence of morphologically abnormal sperm. These data suggest that DEP toxicity may affect the male reproductive system in an AhR-dependent manner.  相似文献   

春季西瓜留瓜个数和整枝方式对产量的影响     

周利利  张飞雪  赵根  陈丽萍  张建明  沈卫新 《蔬菜》2021,(8):23-25

为探究湖州地区春季西瓜高产优质栽培技术,对小型西瓜的不同留瓜个数和中型西瓜的不同整枝方式进行了比较。结果表明:小型瓜单株留2个瓜,中心糖含量可达10.67%,667 m~2产量达2 419.2 t,既保证了西瓜的品质优良,又保证了生产效益;中型瓜可采用双蔓整枝,在保证产量和品质的同时,简化了田间操作,节约了土地。  相似文献   

基于综合视角的县域国土空间冲突测度与功能优化——以江西省宜黄县为例     

向芳芳  黄丽萍  陈荣清 《水土保持通报》2024,44(1):158-171

[目的]测度国土空间冲突强度,分区探讨国土空间冲突管理和优化国土空间利用途径,为县域国土空间可持续开发利用提供新的思路。[方法]以江西省宜黄县为例,通过景观生态指数模型、可开发强度分析、用地现状与适宜性评价叠加3种方法,以“把握景观格局—明确开发强度—摸底适宜性—实现功能优化”综合视角为导向,构建综合视角下国土空间冲突测度模型,识别乡镇尺度下空间显著冲突与区域本位基底相耦合的综合冲突类型区,进而提出功能优化措施。[结果](1)2010—2020年宜黄县城镇化趋势扩大,生态空间的容纳度变低,国土空间主要表现为农业生产空间和生态空间向工矿生产空间和城镇生活空间转移。(2)2010—2020年宜黄县区域空间冲突强度加剧,不同时段和区域下空间冲突强度和分布情况差异显著,可控空间主要分布在南部,轻度和中度冲突在域内分布较为均匀,重度冲突主要分布在北部、中部和东部。(3)根据开发强度和冲突产生诱因,可将宜黄县乡镇划分为地形发展劣势区、城镇开发密集区和自然保护地集中区3类,作为空间功能优化的重点。(4)宜黄县乡镇尺度上空间冲突类型区分布存在差异性,表现为：农业空间与生态空间冲突>城镇空间与生态...  相似文献   

磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化   总被引：1，自引：0，他引：1  

王振英  程罗根  陈宏  彭永康 《作物学报》2003,29(4):545-550

研究了不同浓度APM(4～6 μmol/L)和不同处理时间(4～6 h)对洋葱根尖细胞有丝分裂的影响.结果表明,当用5.6 μmol/L APM处理洋葱分生组织16 h后,中期细胞有丝分裂指数(Met.Ⅰ)从0.8(对照)分别提高至5.0、5.2.结果也同时表明,当APM浓度超过4 μmol/L时,严重影响细胞有丝分裂.在根尖细胞中,多极分裂细胞(尤其是3～4级),中期染色  相似文献   

烤烟主要化学成分与烟叶品质关系研究进展   总被引：14，自引：0，他引：14  

黎根  毕庆文  汪健  王海明  王豹祥  王林  闫铁军 《河北农业科学》2007,11(6):6-9,41

介绍了烟草的研究现状及烤烟主要化学成分与烟叶的外观质量、物理特性、评吸质量、烟气与焦油的关系.  相似文献   

加工番茄果秧分离装置运动过程分析   总被引：4，自引：0，他引：4  

李成松  坎杂  谭洪洋  孙日宾  梁荣庆  冯玉磊 《农业机械学报》2012,43(4):66-69

为了深入研究加工番茄果秧分离特性,分析了加工番茄果秧分离装置激振器的运动过程,利用达朗贝尔原理建立其理论模型。利用SolidWorks对激振器的运动过程进行了仿真,并在FS-35型加工番茄果秧分离试验台上,利用高速摄像机拍摄了分离滚筒的运动过程,利用Blaster’s Mas软件对录像进行了处理和分析。分析结果表明,理论模型与实际运动吻合较好,验证了理论模型的正确性。  相似文献