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1.
Abstract. Ammonia excretion rate of the marine bivalve Tapes decussatus (L.) varied with body weight, temperature and starvation. There was a steady state in the excretion rates, in which these rates neither increased nor decreased during the first 6 days of starvation. The highest rates of ammonia excreted during the steady state (before decline to lower level) depended on the temperature (715 ± 86 and 395 ± 55 μg NH4.N/clam/h × 10−2) at 28°C and 20°C, respectively. At 16°C, the steady state extended from 6h to 18d starvation. Ammonia excretion rates were higher for starved clams than for fed clams at all sizes, e.g. clams of 0·07 g dry flesh weight (28 ± 9 and 13 ± 5 μg NH4.N/clam/h × 10−2 respectively) at 16°C but not at 20°C and 28°C. At each temperature, weight-specific ammonia excretion rates were related to dry flesh weight of starved clams but were not related to fed ones.  相似文献   
2.
The effects of different concentrations of mercury on the cyanobacterium Phormidium fragile were studied. The results revealed that the dry biomass yield, protein content, chlorophyll a and carotenoids decreased with increased concentration of mercury from 0.01 to 1.5 mg L-. Also, malate dehydrogenase (MDH), glutamate dehydrogenase (GIDH) and nitrate reductase activity was suppressed by increased metal concentration whereas glutamic-oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were hardly affected. Mercury uptake increased at the higher levels of the metal. On the other hand, all the amino acid components gradually increased with rise of the metal level except isoleucine, histidine and methionine which were suppressed by the smaller doses and completely disappeared by the larger doses of the metal.  相似文献   
3.
This research deals with the investigating the effect of nanoparticles on the various properties of nanocomposite fabrics produced from melt spinning of various blend ratios of prepared masterbatch containing Ag/TiO2 nanoparticles. The results revealed that the wear properties of modified fabrics improved as compared to pure fabrics with a trend justified considering modulus or crystallinity of fabrics with opposite effects. About 40 % UV protection enhancement has been obtained applying this kind of nanoparticles in the close relationship with the crimp contraction of textured yarns. A considerable improvement in the garment comfort has been recorded for nanocomposite sample containing 1 wt% nanoparticles. The lower permeability at low environment temperature and a higher at higher one, as compared to the pure sample, were obtained using this sample. It is highly interesting that these desirable changes in permeability can be achieved in the range of common environment temperatures (15–35 °C) being adapted to the human body requirements. The changing point is about 25 °C exactly meeting the body requirements by changing environment temperatures. A UV-induced solid state nanocomposite interaction increasing wear properties of UV-irradiated nanocomposite fabrics has been discovered.  相似文献   
4.

Background:

Skin flap grafting is a popular approach for reconstruction of critical skin and underlying soft tissue injuries. In a previous study, we demonstrated the beneficial effects of two 5α-reductase inhibitors, azelaic acid and finasteride, on tissue survival in a rat model of skin flap grafting. In the current study, we investigated the involvement of nitric oxide and inducible nitric oxide synthase (iNOS) in graft survival mediated by these agents.

Methods:

A number of 42 male rats were randomly allocated into six groups: 1, normal saline topical application; 2, azelaic acid (100 mg/flap); 3, finasteride (1 mg/flap); 4, injection of L-NG-nitroarginine methyl ester (L-NAME) (i.p., 20 mg/kg); 5, L-NAME (20 mg/kg, i.p.) + azelaic acid (100 mg/flap, topical); 6, L-NAME (20 mg/kg, i.p.) + finasteride (1 mg/flap, topical). Tissue survival, level of nitric oxide, and iNOS expression in groups were measured.

Results:

Our data revealed that azelaic acid and finasteride significantly increased the expression of iNOS protein and nitric oxide (NO) levels in graft tissue (P < 0.05). These increases in iNOS expression and NO level were associated with higher survival of the graft tissue.

Conclusion:

It appears that alterations of the NO metabolism are implicated in the azelaic acid- and finasteride-mediated survival of the skin flaps.Key Words: Finasteride, Azelaic acid, Surgical flaps, Nitric oxide, Nitric oxide synthase Type II  相似文献   
5.

Purpose

The main objective of this study was to examine the potential of using hyperspectral image analysis for prediction of total carbon (TC), total nitrogen (TN) and their isotope composition (δ13C and δ15N) in forest leaf litterfall samples.

Materials and methods

Hyperspectral images were captured from ground litterfall samples of a natural forest in the spectral range of 400–1700 nm. A partial least-square regression model (PLSR) was used to correlate the relative reflectance spectra with TC, TN, δ13C and δ15N in the litterfall samples. The most important wavelengths were selected using β coefficient, and the final models were developed using the most important wavelengths. The models were, then, tested using an external validation set.

Results and discussion

The results showed that the data of TC and δ13C could not be fitted to the PLSR model, possibly due to small variations observed in the TC and δ13C data. The model, however, was fitted well to TN and δ15N. The cross-validation R2 cv of the models for TN and δ15N were 0.74 and 0.67 with the RMSEcv of 0.53% and 1.07‰, respectively. The external validation R2 ex of the prediction was 0.64 and 0.67, and the RMSEex was 0.53% and 1.19 ‰, for TN and δ15N, respectively. The ratio of performance to deviation (RPD) of the predictions was 1.48 and 1.53, respectively, for TN and δ15N, showing that the models were reliable for the prediction of TN and δ15N in new forest leaf litterfall samples.

Conclusions

The PLSR model was not successful in predicting TC and δ13C in forest leaf litterfall samples using hyperspectral data. The predictions of TN and δ15N values in the external litterfall samples were reliable, and PLSR can be used for future prediction.
  相似文献   
6.
Saturated hydraulic conductivity (Ksat) is regarded as a key soil physical variable to determine soil infiltration rate, percolation depth and other hydrological processes. The purpose of this study was to determine the best soil sampling’s ring size for measuring Ksat. For this purpose, 25 rings with five different diameters (2, 3, 4, 5 and 6 inch) and with the same depth of 20 cm (five replicates) were hammered in close vicinity to each other into the ground of undisturbed loess deposits of a small farm to measure Ksat and bulk density. Hydraulic conductivity was measured at three constant loads of 22, 27 and 32 cm. The results showed that the ring with the internal diameter of 6 inches had the minimum variation coefficient and maximum Ksat for all the three hydraulic heads. In addition, the bulk density of this ring size was less than the other rings.  相似文献   
7.
The one dimensional (1D) Ni-ZnO nanoparticles have been synthesized by a simple hydrothermal method. A novel photocatalyst of nanostructured Ni-ZnO which immobilized on polyacrylonitrile nanofibers were successfully fabricated using electrospinnig technique. The structures of nanofibers were characterized by various techniques including Scanning Electron Microscope (SEM), X-ray powder Diffraction (XRD), Fourier transform infrared (FT-IR), UV-Vis diffuse reflectance (DR) and thermogravimetric analys (TGA). The Ni-ZnO/PAN nanofibers photodegradation efficiency was optimized with factorial design method in order to act highly effective in the photocatalytic degradation of Methyle orange (MO). The highest decolorizing efficiencies using introduced material were achieved by 0.8 g l -1 of catalyst and 10 mg l -1 of MO at natural pH under visible light irradiation. The obtained results exhibited that Ni-ZnO/PAN nanofibers have high visible light photocatalitic activities. Overall, the presented material can be used as an efficient, low cost and healthily secure photocatalyst in the field of water treatment.  相似文献   
8.
This study was performed to investigate the protective and therapeutic effects of resveratrol (RES) against CdCl2-induced toxicity in rat testes. Seven experimental groups of adult male rats were formulated as follows: A) controls+NS, B) control+vehicle (saline solution of hydroxypropyl cyclodextrin), C) RES treated, D) CdCl2+NS, E) CdCl2+vehicle, F) RES followed by CdCl2 and M) CdCl2 followed by RES. At the end of the protocol, serum levels of FSH, LH and testosterone were measured in all groups, and testicular levels of TBARS and superoxide dismutase (SOD) activity were measured. Epididymal semen analysis was performed, and testicular expression of Bcl-2, p53 and Bax was assessed by RT-PCR. Also, histopathological changes of the testes were examined microscopically. Administration of RES before or after cadmium chloride in rats improved semen parameters including count, motility, daily sperm production and morphology, increased serum concentrations of gonadotropins and testosterone, decreased testicular lipid peroxidation and increased SOD activity. RES not only attenuated cadmium chloride-induced testicular histopathology but was also able to protect against the onset of cadmium chloride testicular toxicity. Cadmium chloride downregulated the anti-apoptotic gene Bcl2 and upregulated the expression of pro-apoptotic genes p53 and Bax. Resveratrol protected against and partially reversed cadmium chloride testicular toxicity via upregulation of Bcl2 and downregulation of p53 and Bax gene expression. The antioxidant activity of RES protects against cadmium chloride testicular toxicity and partially reverses its effect via upregulation of BCl2 and downregulation of p53 and Bax expression.  相似文献   
9.
The pharmacokinetics of acetaminophen was investigated following oral dosing to Shiba goats in order to evaluate the properties of gastric emptying. Acetaminophen was intravenously and orally administered at 30 mg/kg body weight to goats using a crossover design with a 3-week washout period. The stability of acetaminophen in rumen juice was also assessed. Acetaminophen concentrations were measured by HPLC. Since acetaminophen was stable in rumen juice for 24 hr, the extremely low bioavailability (16%) was attributed to its hepatic extensive first-pass effect. The mean absorption time and absorption half-life were unexpectedly short (4.93 and 3.35 hr, respectively), indicating its marked absorption from the forestomach, which may have been due to its smaller molecular weight. Therefore, acetaminophen was considered to be unsuitable for evaluating gastric emptying in Shiba goats.  相似文献   
10.

Objective

To examine the maturational competence, embryo development and expression of genes involved in oocyte maturation and cumulus expansion (GDF9, BMP15, HAS2, TNFAIP6, FGF17 and FSHr) following two standard methods of bovine COCs vitrification.

Methods

Bovine cumulus-oocyte complexes (COCs) were aspirated from slaughtered ovaries and then distributed into three groups: non-vitrified COCs (control), vitrification 1 group (V1); vitrification was performed by 15% ethylene glycol (EG) and 15% DMSO in holding media (TCM-199 with 20% FCS); and vitrification 2 group (V2); vitrification was performed by 40% EG in holding media. After vitrification, COCs were warmed in two steps and cultured and then evaluated for nuclear maturation, embryo development and gene expressions.

Results

The mean (±SD) percentages of nuclear maturation and blastocyst/cleaved were higher in control group (79.5 ± 8.0 and 31.0 ± 5.1%) than the V1 (34.8 ± 9.1 and 4.4 ± 5.1%) and V2 (47.8 ± 11.7 and 7.1 ± 5.8%) groups (P < 0.05), respectively. Further, COCs in V2 group showed higher mean (±SD) percentages of cleavage compared to V1 group (31.8 ± 1.0 vs 21.7 ± 2.8%; P < 0.05). GDF9 and BMP15 expression levels were higher in COCs in the control than of the vitrification groups (P < 0.05). In addition, expression level of GDF9 and BMP15 was higher in V2 group than in V1group (P < 0.05). The expression of HAS2 and FGF17 in V1 group was lower (P < 0.05) than that of the V2 groups.

Conclusions

Expression of oocyte maturation genes was affected by vitrification procedure and conditions. Using EG alone for vitrification of bovine immature COCs, resulted in higher expression of GDF9, BMP15 and production of more in vitro matured and cleaved oocytes.
  相似文献   
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