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排序方式: 共有79条查询结果,搜索用时 31 毫秒
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JI Yun HJ Park MH Park MS Kim JH Choi E Lee SP Gong JM Lim ST Lee 《Reproduction in domestic animals》2014,49(5):705-710
Recently, isolation and in vitro culture of putative spermatogonial stem cells (SSCs) in the domestic cat have been conducted. However, the cellular niche conditions that facilitate the establishment and long‐term maintenance of feline SSCs (FSSCs) have not been described. Therefore, we investigated the type of feeder cells used to stimulate colony formation and growth of FSSCs among the various factors in the FSSC niche. Spermatogonial stem cells isolated from feline testes were cultured on mitotically inactivated testicular stromal cells (TSCs) derived from cats, dogs and mice, and mouse embryonic fibroblasts (MEFs). The formation and growth of colonies derived from SSCs cultured on each type of feeder cell were identified at passage 0, and the morphology, alkaline phosphatase (AP) activity and expression of SSC‐specific genes in surviving colonies were investigated at passage 4. Among these diverse feeder cells, TSCs from cat showed the greatest colony formation, growth and maintenance of FSSCs, and SSC colonies cultured by passage 4 showed a typical dome‐shaped morphology, AP activity and expression of SSC‐specific genes (NANOG, OCT4, SOX2 and CD9). Accordingly, these results demonstrate that feline TSCs could be used as feeder cells to support the establishment and maintenance of SSCs from domestic cats. 相似文献
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SHARON STEVENSON dvm ms PhD Diplomateacvs MARVIN L. OLMSTEAD dvm ms Diplomateacvs JOSEPH KOWALSKI dvm PhD 《Veterinary surgery : VS》1986,15(1):99-102
Swab specimens for bacterial culturing were obtained from 82 consecutive dogs and 13 cats undergoing open reduction and internal fixation of 110 fractures. Bacteria were isolated from 51 wounds. When the data were analyzed by stepwise logistic regression, it was found that bacterial contamination of wounds was associated most often with open fractures and with tibia or radius/ulna fractures. Contaminated wounds were five times more likely to develop complications than those without bacterial contamination. Regardless of results of bacterial culturing, postoperative complications developed significantly more often in animals that were febrile (>39.4° C [103° F]). Postoperative complications did not develop in animals that did not have bacterial contamination of wounds and were not febrile. 相似文献
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Distributions of the vector Culicoides brevitarsis Kieffer (Diptera: Ceratopogonidae) (determined from light trap data) and 2 arboviruses (determined from seroconversions in sentinel cattle) were studied in eastern New South Wales in 1993–94. C brevitarsis was recorded progressively from endemic areas on the north coast, to Nowra on the south coast, and westward to Scone, in the Hunter Valley. C brevitarsis also survived through winter at Paterson, in the Hunter Valley. Its apparently focal reappearance in this marginal area had no obvious effect on the broad pattern of its progression or the dispersal of Akabane and bluetongue viruses. These viruses were first recorded from foci near Coffs Harbour, on the mid-north coast. Their first occurrences at different locations were associated with those of C brevitarsis, but not with each other. The viruses were found only within the recorded limits of the vector's distribution. Delays between the initial occurrence of C brevitarsis and first evidence of virus transmissions at locations ranged from 2 to 7 months. The delays decreased away from the points of focus and were negatively associated with the time of initial occurrence of the vector. Seroconversions to the viruses were related to the presence of C brevitarsis. However, the densities of C brevitarsis had no apparent effect on the initial numbers of cattle seroconverting to either virus. The results support the conclusion that the progressions of C brevitarsis and Akabane and bluetongue viruses were the result of gradual movements by the vector. 相似文献
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Osteosarcoma at the Site of a Cortical Bone Allograft in a Dog 总被引:1,自引:0,他引:1
P. B. VASSEUR DVM DiplomateACVS S. STEVENSON DVM PhD DiplomateACVS 《Veterinary surgery : VS》1987,16(1):70-74
An osteosarcoma developed in the proximal femur of a 9-year-old female Great Dane. The femur had been fractured 5 years previously and was stabilized with plate fixation with the incorporation of a frozen cortical bone allograft. The dog recovered without complications after the surgery and had normal use of the leg before the osteosarcoma developed. The tumor had metastasized to the lungs, spleen, and ipsilateral popliteal lymph node. 相似文献
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D. E. STEVENSON 《The Journal of small animal practice》1963,4(5):339-340
Abstract— Congenital diaphragmatic hernia was found in two beagle pups which died shortly after being born. In both cases the small intestine was located in the thoracic cavity. 相似文献
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A. J. STEVENSON M. P. WEBER R. TRUDEL R. LEAVITT‡ D. WOODARD‡ F. TODI M. MENDONCA V. ROBILLO L. YOUNG S. KACEW 《Journal of veterinary pharmacology and therapeutics》1994,17(3):163-168
Analytical procedures were developed to monitor furosemide concentrations in post-race serum and urine samples obtained from horses participating in an exercise-induced pulmonary haemorrhage (EIPH) program. High performance liquid chromatography with ultraviolet light detection proved a reliable, sensitive method for measuring urinary furosemide concentrations up to 12 h after administration of either 150 or 250 mg of the drug to race horses. However, this method was unreliable for determination of serum furosemide concentration. High performance liquid chromatography with fluorescence detection proved a reliable, sensitive method for measuring serum furosemide concentrations in horses administered 250 mg of the diuretic, permitting detection approximately 5–10 ng/ml 6 h after treatment. This method was applied field conditions where furosemide was administered to horses (between 150 and 250 mg intravenously) 4 h prior to the race. Analytical results assisted establishing a threshold concentration of 85 ng/ml for serum furosemide. was found that serum furosemide concentrations are a valid measure of compliance with furosemide administration in the EIPH program. 相似文献
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A review of the clinical and pathologic evaluation of allogeneic bone grafts is presented with particular emphasis upon partial joint replacement. The histologic evaluation of cortical bone transplantation is reviewed. Radiographic evaluation of cortical bone transplants is presented in an attempt to understand the normal events that take place in incorporation of the graft by the host. The histologic and radiographic correlation is related to the healing process. Current methods of graft collection and storage are reviewed. While collection of the graft is fairly uniform, there are many varied treatment and storage procedures. General surgical procedures used in performing full cortical bone grafts are reviewed. The clinical use of an osteoarticular allogeneic bone graft in the elbow of a dog is presented, with a review of the operative and postoperative evaluation of the clinical patient. 相似文献