Isolation and purification of a diagnostic antigen for bovine cysticercosis by hydrophobic chromatography     

E G Hayunga  M P Sumner 《Veterinary immunology and immunopathology》1991,28(1):57-65

An ammonium sulfate fraction of Taenia hydatigena cyst fluid (ThFAS) was further fractionated by hydrophobic interaction chromatography, using alkylagarose and omega-amino alkylagarose columns, in an effort to isolate and purify a specific diagnostic antigen in the ThFAS preparation. The less than 12 kDa antigen was found to have an affinity for immobilized alkanes with chain length of six carbons or greater. The antigen was recovered in an ethylene glycol eluate from a hexylagarose column then analyzed by Western blot; it reacted with bovine and human cysticercosis infection sera and with specific monoclonal antibodies but not with control sera or fascioliasis infection sera. When the eluate was used as coating antigen in a plate ELISA assay no false positive reactions were seen in sera from cattle infected with Fasciola hepatica; false positive reactions were observed for the unfractionated ThFAS antigen preparation.  相似文献   

Application and crop safety parameters for soil fumigants   总被引：2，自引：0，他引：2  

A. S. Csinos  T. M. Webster  D. R. Sumner  A. W. Johnson  C. C. Dowler  K. W. Seebold 《Crop Protection》2002,21(10):973-982

Metam sodium alone and in combination with 1,3-dichloropropene plus 17% chloropicrin (1,3-D+C-17) were evaluated under polyethylene mulch film as alternatives for methyl bromide in tobacco and tomato transplant production for both efficacy against pests and crop safety. Eight different weed species, 10 genera or species of fungi and several agronomic criteria were evaluated at three different sites. In general both the metam sodium alone and in combination with 1,3-D+C-17 were highly efficacious when compared to methyl bromide. Short polyethylene film retention times and short aeration times resulted in poor stands and poor crop vigor while relatively long polyethylene film retention times and long aeration periods at the same rates typically resulted in high stand counts and vigor. Combination treatments were more phytotoxic to germinating seed of tobacco and tomato. Vigor and stand counts of the seedlings were higher as aeration time increased, suggesting phytotoxic residues dissipate with time. Method of application of metam sodium, either injected with chisels or sprayed onto the soil surface and incorporated with a tractor-powered tiller alone or co- applied with 1,3-D+C-17 chisel injected, did not affect the efficacy of the treatments. Caution regarding phytotoxicity must be exercised when seeding into soil fumigated with metam sodium alone or combined with 1,3-D+C-17. Additional work will be required to establish safety periods required prior to transplanting crops into fumigated soil.  相似文献   

In vitro studies of Norwegian Red bovine semen immobilized and cryopreserved in alginate solid gel network          下载免费PDF全文

AH Alm‐Kristiansen  ER Gaustad  G Bai  FB Standerholen  G Klinkenberg  E Kommisrud  KE Waterhouse 《Reproduction in domestic animals》2018,53(2):365-370

Development of new semen cryopreservation techniques improving sperm survival and ensuring availability of viable spermatozoa for a prolonged time‐period after AI is promising tools to reduce sensitivity of timing of AI and enhance overall fertility. The SpermVital^® technology utilizes immobilization of bull spermatozoa in a solid network of alginate gel prior to freezing, which will provide a gradual release of spermatozoa after AI. The objective of this study was to compare post‐thaw sperm quality and in vitro sperm survival over time of Norwegian Red bull semen processed by the SpermVital^® (SV) technology, the first commercialized production line of SpermVital^® (C) and by conventional procedure applying Biladyl^® extender (B). Post‐thaw sperm motility was not significantly different between SV, C and B semen (p > .05). However, sperm viability and acrosome intactness were higher for SV than C and B semen (p < .05). Small differences in DNA quality were observed (p < .05). Sperm viability after storage in uterus ex vivo was higher for SV than for C semen (p < .05). Furthermore, sperm survival in vitro over time at physiological temperature was significantly higher for SV semen than C semen as well as B semen during the incubation period of 48 hr (p < .05). In conclusion, the SpermVital^® technology is improved and is more efficient in conserving post‐thaw sperm quality and results in higher sperm viability over time in vitro for SV than for C and B semen.  相似文献   

Orbital apex syndrome secondary to an orbital sarcoma in a dog     

Jayden Robert  Filipe Espinheira Gomes  Ian Porter  Julia P. Sumner 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(1):27

A 12-year-old castrated male cocker spaniel dog was presented with a 4-week history of left episcleral injection and pawing at the face. Clinical examination findings included left internal and external ophthalmoplegia, left dorsal strabismus, pain opening the mouth, and intermittent amaurosis. Imaging studies revealed a left orbital apex mass with adjacent sphenoid bone lysis and extension into the cranial cavity. A left exenteration was performed and histopathology confirmed an orbital soft tissue sarcoma.Key clinical message:This report describes an orbital tumor causing orbital apex syndrome. This condition should be differentiated from cavernous sinus syndrome as the latter does not course with optic neuropathy.  相似文献   

Influence of locoregional lymph node aspiration cytology vs sentinel lymph node mapping and biopsy on disease stage assignment in dogs with integumentary mast cell tumors     

Janis Lapsley  Galina M. Hayes  Valentin Janvier  Ashleigh W. Newman  Jeanine Peters‐Kennedy  Cheryl Balkman  Julia P. Sumner  Philippa Johnson 《Veterinary surgery : VS》2021,50(1):133-141

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Arsenate Displacement from Fly Ash in Amended Soils   总被引：1，自引：0，他引：1  

Qafoku  Nikolla P.  Kukier  Urszula  Sumner  Malcolm E.  Miller  William P.  Radcliffe  David E. 《Water, air, and soil pollution》1999,114(1-2):185-198

Arsenic (As) is the biggest environment contaminant in most of the soils where fly ash is applied. Usually, it is not mobile and strongly adsorbed on to soil particles. However, in gypsum and phosphorus amended soils As may be much more mobile. A study in repacked columns was conducted to determine whether or not As becomes mobile when Ca(H₂PO₄)₂and CaSO₄are used as leaching solutions, and to compare the competitive interactions between PO₄-AsO₄and SO₄-AsO₄. Arsenic concentration in leachate was found to be approximately ten times greater when Ca(H₂PO₄)₂was used to leach the columns as compared to CaSO₄. A maximum concentration of 800 μg As L^-1was found in the leachate in this case, which is much higher than the groundwater limit of 50 μg L^-1for drinking water established by the United States Environmental Protection Agency. In fly ash, the portion of arsenate non-specifically adsorbed is believed to be much lower than that of specifically adsorbed. Sulfate anions were able to displace only non-specifically adsorbed arsenate. In this case the concentration of As in leachate was found to be within acceptable limits. On the other hand, phosphate can compete with arsenate for all available adsorption sites, non-specific and specific. Phosphate displacement of both forms of arsenates increases As mobility in both control and fly ash treatments.  相似文献   

Soil Ca, Al, acidity and penetration resistance with subsoiling, lime and gypsum treatments   总被引：1，自引：0，他引：1  

J. M. McCray  M. E. Sumner  D. E. Radcliffe  R. L. Clark 《Soil Use and Management》1991,7(4):193-199

Abstract. Crop growth on strongly weathered soils is often limited by soil compaction in addition to aluminium toxicity and/or calcium deficiency. This study examines the effects of subsoiling, lime and gypsum on penetrometer resistance, acidity, aluminium and calcium levels and cotton ( Gossypium hirsutum L.) root growth on soils transitional between Cecil and Appling series (clayey, kaolinitic, thermic Typic Hapludults) in the Piedmont region of Georgia, USA. The main plots were subsoiled to depths of 0.35 or 0.80 m or untreated. Dolomitic limestone (0 or 4.03 t per hectare on subplots) and phosphogypsum (0 or 10 t per hectare on sub-subplots) were incorporated into the surface soil (0.15 m). Deep subsoiling (0.80 m depth) decreased penetrometer resistance at 0.3–0.5 m depth and increased yield in two of three years, but there was no response to shallow subsoiling (0.35 m depth). Lime increased yield when surface soil water pH prior to amendment was less than a Cate-Nelson critical value of 4.6. Gypsum moved downward much more rapidly than lime, increasing soil solution calcium ion activity to a depth of 0.8 m within 5 months of application. There were differences in clay content between replicate plots and calcium movement was faster where the clay content was less. Yield responses to gypsum in 1986 were attributed to increased root growth below 0.2 m resulting from the increased calcium ion activity. Yield response to gypsum in limed sub-subplots was significant only in 1986.  相似文献   

Formulation of Environmentally Sound Waste Mixtures for Land Application     

Schumann  Arnold W.  Sumner  Malcolm E. 《Water, air, and soil pollution》2004,152(1-4):195-217

Major impediments to the land application of coal combustion byproducts (fly ash) for crop fertilization have been the presence of heavy metals and their relatively low and imbalanced essential nutrient concentration. Although nutrient deficiencies, in particular N, P, and K, may be readily augmented by adding organic wastes such as sewage sludge and animal manure, the indiscriminate application of mixtures to crops can cause excessive soil alkalinity, imbalanced nutrition (P, Mg), phytotoxicities (B, Mn, ammonia, nitrite), and unspecified contamination of the food chain by elements such as As. In this study, nutrient availability data and linear programming (LP) were used to solve these problems by formulating fly ash-biosolid triple mixtures which complied with both plant and soil fertilization requirements, and met existing U.S.A. environmental regulations for total As application in sewage sludge (EPA-503). Thirteen different fly ash samples were LP-formulated with sewage sludge, poultry manure, CaCO₃, and KCl to yield 13 unique mixtures, which were then evaluated in greenhouse pot experiments. Results indicated that normal growth and balanced nutrition of sorghum (Sorghumbicolor L.) and soybean (Glycine max (L.) Merr.) crops were achieved in all mixtures, comparable to a balanced fertilizer reference treatment, and significantly better than the untreated control. Phytotoxic levels of B, NH₃, NO₂ ^-, overliming problems, and excessive As levels which were previously encountered from indiscriminate use of these waste materials, were all well controlled by LP-formulated mixtures. Most fly ash quantities in mixtures were limited by either available B (< 4 kg ha^-1) or total As (< 2 kg ha^-1) restrictions during formulation, while the most alkaline fly ash was limited by its high calcium carbonate equivalence (CCE = 53.9%). These results confirmed that fly ash land application should not be at arbitrary fixed rates, but should be variable, depending on the soil, crop, and particularly the fly ash chemistry.  相似文献   

A case-control study to identify farm factors affecting fertility of dairy herds: univariate description of factors     

FB WEBSTER  IJ LEAN  D. KENNEDY  K. PHILLIPS 《Australian veterinary journal》1997,75(4):266-273

Objective To identify farm factors which were associated with reproductive performance in dairy herds in New South Wales.
Procedure A survey was administered by face to face interview to examine the responses of producers drawn from 757 herds, which used the New South Wales Agriculture Department Dairy Herd Improvement scheme. A case-control approach was used to select a total of 126 herds from the first (top group - cases) and fourth quartiles (low group - controls) for intercalving interval.
Results We found that the estimated interval from calving to first mating was significantly different between groups (P = 0.03) and that the groups significantly differed in both their target for interval to first mating (P = 0.02) and their perceived optimum time for first mating (P = 0.04). Other factors associated with a longer intercalving interval included, use of embryo transfer programs (P = 0.08), younger managers (P = 0.02), fewer breedings per day (P = 0.01), a greater number of people detecting heats (P = 0.07), but less hours spent detecting heats while handling the cows (P = 0.11), and a failure to vaccinate bulls for campylobacteriosis (P = 0.14).
Conclusions Managers of herds with poorer reproductive performance did not intend to mate cattle as soon after calving as managers with better reproductive performance, were not as active in seeking veterinary advice on reproduction, and were attempting to treat reproductive diseases and disorders themselves.  相似文献   

Ex Vivo Comparison of the Biomechanical Properties of Hand‐Sewn and Stapled Jejunoileal Anastomoses in Horses     

José L. Bracamonte DVM  DVSc  Diplomate ACVS  Stacy L. Anderson DVM  MVSc  Steven Hendrick DVM  DVSc  Spencer M. Barber DVM  Diplomate ACVS  David Deutscher ASCT  David Sumner PhD  PEng 《Veterinary surgery : VS》2014,43(4):451-458

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