Genetic diversity estimates in Cicer using AFLP analysis   总被引：2，自引：0，他引：2  

T. T. NGUYEN  P. W. J. TAYLOR  R. J. REDDEN  R. FORD 《Plant Breeding》2004,123(2):173-179

Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic variation among cultivated chickpea and wild Cicer relatives. In total, 214 marker loci were assessed, of which 211 were polymorphic (98.6%) across the 95 accessions that represented 17 species of Cicer. The genetic variation within a species was highest in C. pinnatifidum followed by C. reticulatum and lowest in C. macracanthum. Three main species groups were identified by UPGMA clustering using Nei's pair‐wise distance calculations. Group I included the cultivated species C. arietinum, C. reticulatum and C. echinospermum. Within this group, C. reticulatum accessions were clustered closest to the C. arietinum cultivars ‘Lasseter’, ‘Kaniva’ and ‘Bumper’, supporting the hypothesis that C. reticulatum is the most probable progenitor of the cultivated species. Cicer bijugum, C. judaicum and C. pinnatifidum were clustered together creating group II. Group III contained all nine perennial species assessed and two annual species C. yamashitae and C. cuneatum. The genetic distance detected between group I and group III (0.13) was equivalent to the genetic distance detected between group I and group II (the primary and annual tertiary species, respectively; 0.14). This indicated that the perennial tertiary species may be as valuable for increasing variation to incorporate novel germplasm in the cultigen as the annual tertiary species.  相似文献   

A Comparison of Surgical Training with Live Anesthetized Dogs and Cadavers     

LARRY G. CARPENTER DVM    DONALD L. PIERMATTEI DVM  PhD  Dipiomate ACVS    MOWAFAK D. SALMAN BVMS  PhD  Dipiomate ACVPM    E. CHRISTOPHER ORTON DVM  PhD  Dipiomate ACVS    A. WENDELL NELSON DVM  PhD  Dipiomate ACVS    DANIEL D. SMEAK DVM  Dipiomate ACVS    PAUL B. JENNINGS Jr.  VMD  MS  Dipiomate ACVS ROBERT A. TAYLOR DVM  MS  Dipiomate ACVS 《Veterinary surgery : VS》1991,20(6):373-378

Cadavers were compared with live anesthetized dogs for their effectiveness as models for surgical training of veterinary medical students. One group of students was trained using cadavers, and a peer group was trained using live anesthetized dogs. Both groups then performed an intestinal anastomosis using a live subject. The time to completion of the procedure was recorded. The anastomoses and celiotomy closures were evaluated. Each anastomosis was isolated and pressure tested. Reviewers blindly scored each surgical team's performance based on actual inspection of the surgical site and on viewing videotapes of the procedure. The participants' attitudes toward the use of live animals in teaching and research were documented before and after training. No statistically significant differences could be detected between the two groups. The results suggest that some substitution of cadavers for live dogs in surgical training might be feasible.  相似文献   

Experience with Cyclosporin-A After Renal Allografting in Two Dogs Research Note     

C. R. GREGORY dvm  I. M. GOURLEY dvm  PhD  Diplomateacvs  N. J. TAYLOR bs  J. M. CULLEN vmd  A. EVANS dvm  L. J. ISAAC aht  L. D. COWGILL dvm 《Veterinary surgery : VS》1986,15(6):441-443

After renal allografting, cyclosporin-A was administered to one partially nonmatched dog that was followed for 79 days. Cyclosporin-A and prednisolone were administered to one nonmatched dog that was followed for 805 days. Side effects encountered with cyclosporin-A included lymphocytic dermatitis, papillomatosis, bacterial and fungal infections, and B lymphocyte hyperplasia.  相似文献   

Evaluation of some drug combinations for sedation in the dog     

POLLY M. TAYLOR  MICHAEL E. HERRTAGE 《The Journal of small animal practice》1986,27(5):325-333

A double blind trial was performed in order to investigate the effects of some sedatives in the dog. One hundred and forty-two dogs undergoing radiography for the BVA/KC hip dysplasia scheme were sedated with combinations of acepromazine with pethidine or buprenorphine, or with acepromazine alone. The degree of sedation, resistance to manipulation, sensitivity to noise and response to pain were assessed, and arterial blood samples taken for blood gas analysis. In all respects the combinations of acepromazine with buprenorphine or pethidine produced significantly better sedation than acepromazine alone. Pa0₂ and pH were lower and PaCO₂ higher in dogs receiving the combinations compared with those receiving acepromazine alone, but all values were within normal limits. It was concluded that combinations of pethidine or buprenorphine with acpromazine provide extremely effective and safe sedation in the dog.  相似文献   

Intracellular localization of labeled nucleic acid determined by autoradiographs     

TAYLOR JH 《Science (New York, N.Y.)》1953,118(3071):555-557

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Analysis of phenylbutazone and its metabolites by high performance liquid chromatography     

J. B. TAYLOR  P. LEES  E. L. GERRING 《Equine veterinary journal》1981,13(3):201-203

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Preliminary field trials with 2,4-D ester, barban and tri-allate applied in spray volumes of 5–20 l/ha     

W. A. TAYLOR  C. R. MERRITT 《Weed Research》1974,14(4):245-250

A machine is described for experimental application of herbicides in volumes of spray liquid in the range 5–20 I/ha and at a uniform drop size. Uniformity of distribution is comparable to that from a conventional small-plot sprayer working under optimal conditions. The machine was used to apply 2,4-D ester, barban and tri-allate. Subsequent biological assessments revealed no major loss of effectiveness in weed control when compared with applications in 165 or 200 I/ha sprayed through hydraulic fan nozzles as in contemporary field practice. Essais préliminaires au champ avec l'ester du 2,4-D, le barbane et le triallate appliqués en pulvérisation avec un volume de 5 à 20 l/ha Un appareil est décrit pour l'application expérimental d'herbicides en pulvérisation avec un volume de liquide alla de 5 à 20 I/ha et une taille uniforme de gouttes. L'uniformité de la distribution est comparable à celle d'un pulvérisateur pour petites parcelles travaillant dans des conditions optimales. L'appareil a été utilisé pour appliquer du 2.4-D ester, du barbane et du triallate. Des essais biologiques effectués & la suite des traitements ont montré qu'il n'y avait pas de perte importante d'efficacité herbicide par comparaison avec des traitements appliqués avec 165 ou 200 1/ha en utilisant les buses S fente en usage dans la pratique actuelle des traitements au champ. Vorläufige Feldversuche mit 2,4-D Ester, Barban und Triallat mit Brühemengen von 5–20 l/ha Es wird ein GerSt beschrieben mit dem versuchsmässig Herbizide mit Brühemengen zwischen 5 und 20 1/ha bei gleichbleibender Tropfengrösse ausgebracht werden können. Die Gleichmässigkeit der Verteilung lässt sich mit der eines unter optimalen Bedingungen arbeitenden Parzellenspritz-geräts vergleichen. Mit diesem Gerät wurden 2,4-D Ester, Barban und Triallat ausgebracht. Die darauffotgende bio-logische Früfung zeigte keinen wesentlichen Verlust in der Wirksamkeit der Unkrautbekämpfung, verglichen mit den gegenwärtig üblichen Verfahren mit 163 oder 200 l/ha und Flachstrahldüsen.  相似文献   

Oral transmission of transmissible gastroenteritis virus by muscle and lymph node from slaughtered pigs     

DR COOK  HT HILL†  JD TAYLOR 《Australian veterinary journal》1991,68(2):68-70

A study was conducted in the USA to determine whether transmissible gastroenteritis (TGE) virus could be transmitted from carcases of slaughtered pigs. Transmissible gastroenteritis virus was transmitted to 6-day-old piglets by dosing with homogenates of muscle and lymph node collected from 500 clinically normal pigs at the time of slaughter. All piglets in 2 separately housed litters showed clinical signs of TGE with 5 piglets dying within 10 d of oral dosing with homogenates. Transmissible gastroenteritis virus was isolated from 2 of these piglets and all piglets developed TGE antibody. Transmissible gastroenteritis virus was not isolated in tissue culture from muscle and lymph node homogenates, but was isolated from 4 (0.8%) of 500 tonsil samples collected from the same pigs. A survey of 250 serum samples provided an estimate of the prevalence of slaughtered pigs with TGE antibody of 34.8% in the sample population. The results indicate that carcases of some pigs from TGE endemic areas contain viable TGE virus, and that there would be a substantial risk of introducing TGE virus into Australia by the importation of uncooked pig meat from these areas.  相似文献   

DETERMINATION OF MOISTURE CONTENT WITH A GAMMA BACKSCATTER DENSITY GAUGE     

O. CIFTCIOGLU  D. A. BYATT  D. TAYLOR 《European Journal of Soil Science》1972,23(1):32-37

Gamma-radiation, backscatter gauges, as normally employed for the determina-tion of the bulk density of soils, use integral mode counting. They are relatively insensitive to the presence of soil moisture and hence not generally suitable for monitoring soil moisture content, particularly, if the soil type effect is minimized by counting only gamma-photons of energy > 0.400 MeV for a ¹³⁷Cs source. However, using differential mode counting with the window set at about 0.08 MeV, the phenomenon is one of multiple instead of single scattering. With a source-detector Separation of the order of 20 cm, about eight Compton in-teractions are obtained and, as a multiplying factor of ZjA is involved at each interaction, the increased response with hydrogen-containing material is easily observable. The value of Z/A for hydrogen is 1.0, and for materials not containing hydrogen about 0.5; therefore for water it is 5/9. Hence under these conditions, the response for water compared with non-hydrogen-containing materials is [(s/9)/(1/2)]⁸= 2.32 times, an increase of 132 per cent. This method can therefore be exploited to measure the moisture content of soils. The sensitivity is high, and the accuracy is better than ± 5 per cent, which is not as good as can be achieved with a separate thermal neutron detector, but is useful for many purposes and, of course, only one instrument is required.  相似文献   

THE SPATIAL RESPONSE PATTERN OF GAMMA BACKSCATTER DENSITY GAUGES     

G. DEVLIN  D. TAYLOR 《European Journal of Soil Science》1970,21(2):297-303

This theoretical study shows that the sensitivity of the usual type of gamma backscatter gauge changes markedly for different soil volume elements according to their position with respect to the source and detector locations. There is a maximum in the so-called detector region and a less important maximum in the source region. Calculation shows that 50 per cent of the total response of the instrument originates from 14 per cent by volume of the soil from a hemisphere of diameter d, d being the source-detector separation. Since the response pattern of this type of backscatter gauge is far from uniform, it is necessary to consider the response patterns in planning the conditions of experiments involving these gauges to obtain the highest sensitivity. This is of particular importance in studying soils of a heterogeneous nature.  相似文献