Comparison of three in vitro techniques to estimate benzimidazole resistance in Haemonchus contortus of sheep     

M V Johansen  P J Waller 《Veterinary parasitology》1989,34(3):213-221

Three in vitro assays to detect benzimidazole resistance, namely, the egg-hatch assay, tubulin-binding assay, and a larval-development assay, were evaluated by estimating the level of benzimidazole resistance in three field isolates of Haemonchus contortus compared with a susceptible reference strain. Comparisons were also made with estimates of benzimidazole resistance of the three field strains obtained from an in vivo controlled anthelmintic efficacy test. All three in vitro tests showed similar, consistent results which also suggested greater sensitivity than the in vivo assay. These results indicate that selection of an in vitro technique to determine benzimidazole resistance should therefore be based on considerations other than precision, such as technical expertise, availability of equipment, cost and speed in which diagnosis is required.  相似文献   

Prospects for biological control of nematode parasites of ruminants   总被引：2，自引：0，他引：2  

Waller PJ 《New Zealand veterinary journal》1992,40(1):1-3

Control of nematode parasites in ruminants is directed almost exclusively at the parasitic stage in the host using conventional anthelmintic therapy. However, to complete their life cycle, parasites have to develop through a series of free-living stages on pasture. It is within this environment that there is a vast range of cohabiting micro-organisms, some of which are known to infect or prey on nematodes. These include bacteria, protozoa, viruses and fungi. To date, most interest has been focused on those organisms producing chemical toxins which have been developed as anthelmintics, e.g. Streptomyces/avermectins. Yet the potential of predacious and nematoxic fungi and bacteria for the biological control of nematode parasites of livestock remains largely untapped. These organisms may well offer a cheaper and more sustainable approach to reducing the impact of parasitism in livestock.  相似文献   

Modified live Streptococcus equi ('strangles') vaccination followed by clinically adverse reactions associated with bacterial replication     

Kemp-Symonds J  Kemble T  Waller A 《Equine veterinary journal》2007,39(3):284-286

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Infiltration in surface irrigated swelling soils     

P. M. Waller  W. W. Wallender 《Irrigation and Drainage Systems》1991,5(3):249-266

Infiltration characteristics for border strip irrigation at two sites with swelling clay soils were examined. Volume infiltrated was calculated from flow onto the field monitored with flow meters; depth of water in the soil estimated from soil samples taken before and after irrigation; and the advance profile which was used to calculate the volume infiltrated with time. Volume infiltrated was compared with volume of cracks before irrigation.Linear advance and observed crack closing supported the hypothesis that infiltration approached zero after about 10 min. Volume of cracks was less than 20% of the volume infiltrated. Wetting front was 3–10 times greater than depth of observed surface cracks. There was no significant correlation between intake opportunity time and depth of infiltration, but elevation irregularities were related to infiltration.  相似文献   

Plants as de-worming agents of livestock in the Nordic countries: historical perspective, popular beliefs and prospects for the future.     

PJ Waller  G Bernes  SM Thamsborg  A Sukura  SH Richter  K Ingebrigtsen  J H?glund 《Acta veterinaria Scandinavica》2001,42(1):31-44

Preparations derived from plants were the original therapeutic interventions used by man to control diseases (including parasites), both within humans and livestock. Development of herbal products depended upon local botanical flora with the result that different remedies tended to develop in different parts of the world. Nevertheless, in some instances, the same or related plants were used over wide geographic regions, which also was the result of communication and/or the importation of plant material of high repute. Thus, the Nordic countries have an ancient, rich and diverse history of plant derived anthelmintic medications for human and animal use. Although some of the more commonly used herbal de-wormers were derived from imported plants, or their products, many are from endemic plants or those that thrive in the Scandinavian environment. With the advent of the modern chemotherapeutic era, and the discovery, development and marketing of a seemingly unlimited variety of highly efficacious, safe synthetic chemicals with very wide spectra of activities, herbal remedies virtually disappeared from the consciousness--at least in the Western world. This attitude is now rapidly changing. There is a widespread resurgence in natural product medication, driven by major threats posed by multi-resistant pest, or disease, organisms and the diminishing public perceptions that synthetic chemicals are the panacea to health and disease control. This review attempts to provide a comprehensive account of the depth of historical Nordic information available on herbal de-wormers, with emphasis on livestock and to provide some insights on potentially rewarding areas of "re-discovery" and scientific evaluation in this field.  相似文献   

Effects of helminth infection on the pre-weaning production of ewes and lambs: evaluation of pre- and post-lambing drenching and provision of safe lambing pasture     

P J Waller  J R Donnelly  R J Dobson  A D Donald  A Axelsen  F H Morley 《Australian veterinary journal》1987,64(11):339-343

The pre-weaning production of ewes and lambs in spring on pastures grazed during the previous autumn and early winter by either weaner sheep, adult sheep, or cattle was investigated together with estimating the benefits of pre- and post-lambing drenches to ewes. These treatments were compared with one in which parasitism was uncontrolled in ewes lambing on pastures grazed previously by untreated weaner sheep, and another where parasitism was suppressed by 2-weekly drenching of the weaner sheep and also of the ewes and their lambs. Prior grazing by cattle effectively eliminated infection of pastures with intestinal Trichostrongylus and Nematodirus spp, but less so for Ostertagia spp. Worthwhile reductions in contamination were also achieved by grazing by adult sheep compared with grazing by undrenched weaners. Despite differences in the parasitological status of the pastures, there were no indications that pre-weaning growth rates of lambs were affected. However, wool growth in ewes was reduced by 10 to 20% by parasite infection. Wool growth of ewes on pastures grazed by cattle during the pre-experimental period exceeded that on any other treatment, and was significantly greater than that of ewes on pastures grazed by undrenched weaners. There was no production benefit in giving a pre-lambing drench to ewes on plots contaminated by weaners, or in giving the additional post-lambing drench to ewes grazing on plots contaminated by weaners, adult sheep or cattle.  相似文献   

Pesticide usage on bananas with special references to fungicides     

James Waller 《Pest management science》1978,9(5):478-482

Bananas (including plantains) are widely grown throughout the tropics both for local consumption and for export. Pesticide usage is confined largely to the export crop because of its value and the susceptibility to diseases of varieties suitable for the export market. Most of the bananas grown for export are produced in Latin America and the Caribbean. Weed control with herbicides is particularly valuable in young banana plantations before a mature canopy is produced and when weeds compete with the shallow rooting system. Insecticides are used to control high populations of the banana weevil and may be used against fruit thrips. Nematicides are used extensively to control the banana root nematode and granular applications are now replacing soil injections. Fungicidal control of Sigatoka leaf diseases and fruit diseases has been practised for many years and is essential for the production of high quality, exportgrade fruit. Changes in the chemicals used and in application techniques have brought important benefits in recent years and genetic resistance is likely to play a large part in disease control in the future.  相似文献   

Intramammary infusion of beta1,3-glucan for prevention and treatment of Staphylococcus aureus mastitis     

Persson Waller K  Grönlund U  Johannisson A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(3):121-127

Udder health problems associated with Staphylococcus aureus infections in dairy cows are difficult to control and antibiotics have limited effects. Lately, more interest has been directed towards ways to stimulate the innate immune mechanisms of the animal for better prevention and treatment of mastitis. The objectives of this study were to investigate if intramammary infusion at drying off with the immune modulator beta1,3-glucan can make the udder more resistant to experimental intra mammary S. aureus infection at this time, and to study if intramammary infusion of beta1,3-glucan into lactating udder quarters with chronic subclinical S. aureus infection can stimulate the clearing of the infection. Another aim was to evaluate the effect of beta1,3-glucan on the expression of major histocompatibility complex (MHC class II) on mammary leucocytes, measured by flow cytometry, during these circumstances. The results indicated a slight, but not statistically significant, positive effect of beta1,3-glucan at drying off on the clinical and anti-bacterial response to S. aureus infection, but no therapeutic effect of beta1,3-glucan treatment of udder quarters with chronic subclinical S. aureus mastitis. However, the proportion of MHCII+ milk lymphocytes tended to increase after glucan infusion in those udder quarters indicating a stimulation of the antigen presenting ability. To further evaluate a possible preventive effect of beta1,3-glucan infusion at drying off more studies are needed involving a larger number of animals.  相似文献   

Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis   总被引：7，自引：0，他引：7  

Persson Waller K  Colditz IG  Lun S  Ostensson K 《Research in veterinary science》2003,74(1):31-36

Cytokine kinetics were examined in milk and in afferent and efferent lymph of the supramammary lymph node after intramammary infusion of endotoxin from Escherichia coli. Cows were sampled 0, 2 and 4h after infusion (p.i.). Neutrophils appeared in afferent lymph 2h p.i., and in efferent lymph and milk 4h p.i. The milk contained high concentrations of interleukin-8 (IL-8) at 2 and 4h p.i. IL-8 was also found in lymph, but at lower concentrations. The tumor necrosis factor-alpha (TNF-alpha) concentration tended to increase in afferent lymph at 2h p.i., and increased in milk at 4h p.i. The level of IL-1beta increased at 4h p.i. in milk, but was not detected in lymph. Interferon-gamma was not detected in any sample, at any time. The results indicate a primary role for IL-8 in the recruitment of neutrophils into the gland, and suggest that IL-1beta and TNF-alpha are not necessary for IL-8 production and release in response to endotoxin.  相似文献   

Disintegration of dung pats from cattle treated with the ivermectin anthelmintic bolus, or the biocontrol agent Duddingtonia flagrans     

Dimander SO  Höglund J  Waller PJ 《Acta veterinaria Scandinavica》2003,44(3-4):171-180

An experiment was performed during the grazing seasons of 1998, 1999 and 2000 to study the influence of the antiparasitic drug ivermectin and the nematophagous fungus Duddingtonia flagrans on cattle dung disintegration. The faeces originated from groups of animals that were part of a separate grazing experiment where different control strategies for nematode parasite infections were investigated. Each group consisted of 10 first-season grazing cattle that were either untreated, treated with the ivermectin sustained-release bolus, or fed chlamydospores of D. flagrans. Faeces were collected monthly on 4 occasions and out of pooled faeces from each group, 4 artificial 1 kg dung pats were prepared and deposited on nylon mesh on an enclosed pasture and protected from birds. The position of the new set of pats was repeated throughout the 3 years of the study. Each year, the dung pats were weighed 4, 6, 8 and 10 weeks after deposition and immediately afterwards replaced to their initial positions. Results showed that there was no difference in faecal pat disintegration between groups. However, the time-lag between deposition and complete disintegration of the faeces varied significantly between deposition occasions. Dung pats disappeared within 2 weeks (visual observation) when subjected to heavy rainfall early after deposition, whereas an extended dry period coincided with faeces still remaining 12 months after deposition.  相似文献