猪肺炎支原体黏附因子基因R1R2区的克隆及表达   总被引：4，自引：0，他引：4  

张映  贾广乐  邵国青  聂向庭 《中国兽医学报》2003,23(4):338-341

根据GenBank登录的猪肺炎支原体232株P97基因和J株黏附因子基因设计了1对引物，以我国猪肺炎支原体Z株(强毒株)基因组DNA为模板，通过PCR方法扩增了该株黏附因子基因的部分序列。经序列分析后，重新设计了1对带有EcoRI和HindⅢ酶切位点的引物，并经引物的定点突变，PCR扩增了Z株黏附因子的R1R2区。扩增产物经双酶切后克隆到表达载体pET-32(a) 中。该重组质粒经酶切鉴定后，将其具有正确阅读框架的重组质粒转化到大肠杆菌BL21(DE3)感受态细胞，37℃下经IPTG诱导表达，得到相对分子质量约29000的融合蛋白，表达量约为11％。  相似文献   

Factors affecting the success rate of treatment of recumbent dairy cows suffering from hypocalcaemia     

Gelfert CC  Alpers I  Dallmeyer M  Decker M  Hüting A  Lesch S  Baumgartner W  Staufenbiel R 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2007,54(4):191-198

We aimed to investigate the ratio of accompanying diseases in cows suffering from clinical hypocalcaemia and their influence on cure rate. In five veterinary practices in different regions of Germany, all recumbent cows around parturition were included in the study for a period of 1 year. After recording the case history a clinical examination was done and a serum sample was taken to measure the concentrations of calcium and phosphorus magnesium, beta-hydroxybutyrate, total bilirubin, cholesterol, urea, and the activities of ASAT, CK, and GLDH. Only cows with hypocalcaemia entered the statistical analysis. Hypocalcaemia was the major cause of recumbency in cows of the second lactation or elder. Muscle damage was the second frequent diagnose in recumbent cows and the major concomitant disease in hypocalcaemic cows. The overall cure rate was between 89.4% and 94.8%. Calcium and phosphorus concentrations did not have an influence on cure rate. Non-cured cows had higher serum activities of CK (p<0.043) and ASAT (p<0.006). Nevertheless, the activities of CK and ASAT were no good predictors of treatment failure because of their low specificity and the high cure rate of the cows in the five practices.  相似文献   

Design of the Virtual Instrument of Frequency/Amplitude Sweep     

XIE Ting ting  TANG Bao ping  QIN Shu ren 《保鲜与加工》2002,(4):11-14

In this paper the inevitable trend of constructing open ecosystem in modern settlement environment was discussed through the history of the settlement environment and the characters of modern settlement environment.The ecological crisis,change of the modern life and the citizens' needs and the ecological system character show,that it is important for sustainable development of the settlement environment to construct open ecosystem.The construction of modern habitation and the city room embodies the concept of open ecosystem.Then,in construction and research of modern settlement,the ecological viewpoint and method should be applied.  相似文献   

Effect of Western Architectural Culture on the Traditional Dwellings in Meizhou     

LI Ting - ting 《保鲜与加工》2006,(1):35-39

Under the special social background and source of history, the western architectural culture affected the traditional dwellings in Meizhou from the 19th century till the 20th century. The framework, outline and materials of traditional dwellings were changed in certain extent while the form of plane layout and interior decoration still stood stable maintaining the native characters. This article analyzes the phenomena of incorporation of things with diverse nature.  相似文献   

玉树县草地退化现状及治理措施     

都耀庭  张东杰 《青海草业》2006,15(4):35-37,28

通过调查资料分析,玉树县现有退化草地597533.3hm2,占可利用草场面积的43.56%。草地退化的主要原因是:超载过牧,鼠虫危害以及滥采乱挖等人为因素。并提出了加强草地建设,实施退耕还林(草),退牧还草和三江源生态保护治理等项目工程以及逐步完善和实行以法治草,建立人工草地等措施。  相似文献   

南方红豆杉人工林木材管胞形态特征研究   总被引：1，自引：0，他引：1  

王挺良  林金国  陈天文 《林业勘察设计》2002,(1):9-10

通过对南方红豆杉人工林木材管胞形态的制定和分析,揭示了南方红豆杉木材管胞形态特征及其随轮龄的变化规律。结果表明:南方红豆杉木材管胞长度和长宽比随着轮龄的增大而增大,至32～34年生以后趋于平稳,南方红豆杉人工林的工艺成熟期为32～34年,南方红豆杉人工林木材可作为林产工业的纤维材料使用。  相似文献   

Evaluation of embryonic age and the effects of different proteases on the isolation and primary culture of chicken intestinal epithelial cells in vitro          下载免费PDF全文

Chao Yuan  Qiang He  Jun‐ming Li  Mahmoud Mostafa Azzam  Jian‐jun Lu  Xiao‐ting Zou 《Animal Science Journal》2015,86(6):588-594

The present study evaluates the effects of embryonic age and proteolytic enzymes on the isolation and primary culture of chicken enterocyte and to establish an effective technique for chicken intestinal epithelial cell (IEC) cultivation. Fourteen‐day‐old, 16‐day‐old and 18‐day‐old embryos (average weight: 52.23 ± 0.76 g, 50.86 ± 0.99 g, 48.98 ± 1.03 g) were the source for preparation of enterocyte culture, and trypsin‐ethylene diamine tetraacetic acid, collagenase, thermolysin and combination of collagenase and thermolysin were used for digestion medium. Optimal culture protocols were determined by qualitative assays of proliferation. Cells isolated by using 14‐day‐old embryo and collagenase obtain the best attachment and growth in culture, and the production of continuously growing IEC cultures. Thus, we conclude that the use of collagenase as a dissociating enzyme and 14‐day‐old embryo as a source can be advantageously applied to the isolation of chicken IEC and this method may be useful for various applications and basic studies of the intestinal tract concerning such objects as physiology, immunology and toxicology.  相似文献   

不同药剂预防白蚁蛀蚀木材的效果测定   总被引：2，自引：0，他引：2  

陈红梅  朱建华  张再福  张华庭  陈国顺 《福建林业科技》2000,27(4):45-48

室内采用河砂木块法研究了 15种药剂对白蚁的防治效果 ,48%毒死蜱、5 %锐劲特、48%毒死蜱 + 10 %安绿宝、2 5 %辛硫·灭扫利、5 %锐劲特 + 76 9%樟脑油、10 %安绿宝等 6种药剂 (或混配剂 )抗白蚁蛀蚀效果显著优于氯丹。野外试验了15种药剂 ,2 2个月后的结果表明 :48%毒死蜱单独使用或与 76 9%松节油等混配使用 ,可以保持木材近 2a仍不受白蚁蛀蚀 ;5 %锐劲特、2 5 %辛硫·灭扫利等药剂单独使用或与巴丹等混配使用 ,其效果也优于 70 %氯丹  相似文献   

高校图书馆与高校档案馆数字化建设比较研究     

梅婷 《农业网络信息》2013,(4):57-60

论文从理论研究和实践过程这两个方面,详细讨论了高校图书馆与高校档案馆数字化建设的异同。  相似文献   

Bio—G除臭剂对蛋鸡舍内氨气及产蛋性能的影响   总被引：2，自引：0，他引：2  

林英庭  周海波等 《家畜生态》2002,23(2):26-27

在面积相等，饲养密度相近的2栋蛋鸡舍内连续15d喷洒Bio-G除臭剂，每天1次，每次5L，结果表明，喷洒Bio-G后，鸡舍氨气含量明显下降，但对蛋鸡产蛋性能无明显影响。  相似文献