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Matthew D. Wilson Adelina Latinovic Noel W. Davies Peter B. McQuillan Robert C. Menary 《Journal of Crop Improvement》2018,32(3):331-352
Tasmanian Mountain Pepper is a new crop that produces a valuable flavoring agent derived from its fruit. However, annual yields are unpredictable and its commercial prospects depend upon a reliable fruit set. As poor pollination is a possible cause, the pollination biology of this dioecious plant was examined in natural and plantation settings using direct observations, sticky traps, gas chromatography, and microscopy. A community of small insects visits the flowers, and the insect species represent at least seven families of small flies, three families of beetles, and various wasps and moths. Pollen grains were observed on the bodies of bibionid flies (Bibionidae), syrphid flies (Syrphidae), and snail-flies (Sciomyzidae). The tetrahedral tetrad pollen grains are unlikely to be suitable for dispersal by wind. Similar volatiles were emitted by both males and females; several ocimene compounds are attractants for insect pollen vectors. The female flowers also emitted indole, a known fly attractant. It is therefore likely that Tasmannia lanceolata is dependent on the services of diverse insects, especially Diptera, for promoting fruit set. Plantations should be established in the vicinity of biodiverse native vegetation that supports a wide variety of native flying insects capable of transferring pollen. 相似文献
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Gama A Paredes J Gärtner F Alves A Schmitt F 《Veterinary journal (London, England : 1997)》2008,177(1):45-53
Cadherin-catenin complexes play a critical role in intercellular adhesion, and their altered expression has been implicated in tumour progression. In this study, the expression of E-cadherin, P-cadherin and beta-catenin was analysed in 65 canine malignant mammary tumours and correlated with clinicopathological parameters, proliferation and survival. Reduction in E-cadherin expression was significantly associated with increased tumour size, high histological and invasion grades, lymph node metastasis and high mitotic index. Reduced beta-catenin expression was associated with high histological and invasion grades. Anomalous expression of P-cadherin was only associated with invasion. In 39 cases for which follow-up data were available, reduced E-cadherin and beta-catenin expression was significantly associated with shorter overall survival and disease free survival. Abnormal expression of adhesion molecules is a common phenomenon in canine mammary malignant tumours and may play a central role in tumour progression. 相似文献
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Enikő Laczi Emil Luca Adelina Dumitraş Alexandra Hoaghia Păuniţa Boancă 《Communications in Soil Science and Plant Analysis》2017,48(1):63-72
Chinese cabbage is a leafy green with great economic potential, mostly in those areas where it has not yet been widely cultivated, but its demand is increasing, due to its high nutritional and therapeutic value. The experimental factors involved three cultivars of Chinese cabbage, different irrigation, and fertilization treatments. The main aims of the present research were to establish the influence of water and fertilization management on Chinese cabbage chemical composition in an organic cropping system and to determine relations between the organic fertilizers and cabbage leaf macroelements, essential microelements, and heavy metal concentrations. The results showed that sheep manure was richer in almost all the studied elements, including heavy metals; plants fertilized with this manure have higher levels of heavy metals; irrigation had a secondary effect on the decreasing heavy metal concentration in plants; and plants fertilized with biofertilizers showed high amounts of potassium (K), magnesium (Mg), sodium (Na), copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn). 相似文献
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Héctor Mendoza Guzmán Adelina de la Jara Valido Laura Carmona Duarte Karen Freijanes Presmanes 《Aquaculture International》2010,18(2):189-199
The present work shows the possibility of determining variations in the lipid composition in Tetraselmis suecica under different conditions of culture by means of flow cytometry in cells marked with Nile Red (NR). A significant correlation
was observed between the cellular contents in polar and neutral lipids and the cytometric signal of the marked cells. Likewise,
there was a significant correlation between the ratio of polar and neutral lipids, estimated by cytometry, and the relative
composition of polyunsaturated fatty acids (PUFAs) in Tetraselmis, which corresponded to the greater content of PUFAs detected in the polar lipid fraction of this microalgae. This relationship
between the polar/neutral ratio and the relative contents of PUFAs, together with the flow cytometry and the marking by means
of NR, would make it possible to have an effective indicator of the abundance of PUFAs in Tetraselmis, as well as the development of techniques of massive screening of strains which are hyperproductive of PUFAs and of rapid
checking of the variations in lipid composition in response to cultivation conditions, which are much simpler and more rapid
than traditional techniques. 相似文献
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Genetic variation among five elite winter barley cultivars (H. vulgare L.) currently grown in Bulgaria was assessed at the molecular level using restriction fragment length polymorphism (RFLP)
and randomly amplified polymorphic DNA (RAPD) markers. The present study sampled RFLPs in four well characterized multigene
families in barley: the seed storage protein loci; the 18S, 5.8S and 26S ribosomal DNA loci; the loci coding for 5S ribosomal
RNA and the loci coding subunit α of ATP-A complex in the mitochondrial genome. RFLPs were detected in three out of five investigated
chromosomal loci in the barley cultivars studied. RAPD assay using arbitrary 10-base primers was applied to generate amplified
length polymorphic markers in barley. Overall a total of 15 polymorphic phenotypes were found among the studied barley cultivars
by using 11 out of 25 tested primers. All RAPDs were considered as dominant genetic markers except for two, where PCR and
Southern blot
analysis indicated the presence of codominant amplification products. Five RAPD polymorphisms in F1 and F2 progenies of the cross between Alpha and Obzor were inherited in Mendelian fashion. The determined values for the genetic
variation proved a high genetic similarity among the tested cultivars. Genetic similarity (GS) calculated from RFLP and RAPD data ranged from 0.888 to 0.997 with a mean GS – 0.933.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Zheng M Jagota A Strano MS Santos AP Barone P Chou SG Diner BA Dresselhaus MS McLean RS Onoa GB Samsonidze GG Semke ED Usrey M Walls DJ 《Science (New York, N.Y.)》2003,302(5650):1545-1548
Wrapping of carbon nanotubes (CNTs) by single-stranded DNA (ssDNA) was found to be sequence-dependent. A systematic search of the ssDNA library selected a sequence d(GT)n, n = 10 to 45 that self-assembles into a helical structure around individual nanotubes in such a way that the electrostatics of the DNA-CNT hybrid depends on tube diameter and electronic properties, enabling nanotube separation by anion exchange chromatography. Optical absorption and Raman spectroscopy show that early fractions are enriched in the smaller diameter and metallic tubes, whereas late fractions are enriched in the larger diameter and semiconducting tubes. 相似文献
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Pazos M da Rocha AP Roepstorff P Rogowska-Wrzesinska A 《Journal of agricultural and food chemistry》2011,59(14):7962-7977
Protein oxidation in fish meat is considered to affect negatively the muscle texture. An important source of free radicals taking part in this process is Fenton's reaction dependent on ferrous ions present in the tissue. The aim of this study was to investigate the susceptibility of cod muscle proteins in sarcoplasmic and myofibril fractions to in vitro metal-catalyzed oxidation and to point out protein candidates that might play a major role in the deterioration of fish quality. Extracted control proteins and proteins subjected to free radicals generated by Fe(II)/ascorbate mixture were labeled with fluorescein-5-thiosemicarbazide (FTSC) to tag carbonyl groups and separated by two-dimensional gel electrophoresis. Consecutive visualization of protein carbonyl levels by capturing the FTSC signal and total protein levels by capturing the SyproRuby staining signal allowed us to quantify the relative change in protein carbonyl levels corrected for changes in protein content. Proteins were identified using MALDI-TOF/TOF mass spectrometry and homology-based searches. The results show that freshly extracted cod muscle proteins exhibit a detectable carbonylation background and that the incubation with Fe(II)/ascorbate triggers a further oxidation of both sarcoplasmic and myofibril proteins. Different proteins exhibited various degrees of sensitivity to oxidation processes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), nucleoside diphosphate kinase B (NDK), triosephosphate isomerase, phosphoglycerate mutase, lactate dehydrogenase, creatine kinase, and enolase were the sarcoplasmic proteins most vulnerable to ferrous-catalyzed oxidation. Moreover, NDK, phosphoglycerate mutase, and GAPDH were identified in several spots differing by their pI, and those forms showed different susceptibilities to metal-catalyzed oxidation, indicating that post-translational modifications may change the resistance of proteins to oxidative damage. The Fe(II)/ascorbate treatment significantly increased carbonylation of important structural proteins in fish muscle, mainly actin and myosin, and degradation products of those proteins were observed, some of them exhibiting increased carbonylation levels. 相似文献
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